The expression of keratan sulfate in malignant melanoma enhances the adhesion and invasion activity of melanoma cells.
cell adhesion
glycan
invasion
keratan sulfate
melanoma
paxillin
Journal
The Journal of dermatology
ISSN: 1346-8138
Titre abrégé: J Dermatol
Pays: England
ID NLM: 7600545
Informations de publication
Date de publication:
Oct 2022
Oct 2022
Historique:
revised:
29
05
2022
received:
03
01
2022
accepted:
13
06
2022
pubmed:
12
7
2022
medline:
12
10
2022
entrez:
11
7
2022
Statut:
ppublish
Résumé
Mammals express a wide variety of glycans that include N-glycans, O-glycans, proteoglycans, glycolipids, etc. Glycan expression can modulate the cellular functions, and hence is strongly involved in the onset and progression of numerous diseases. Here, we report the relevance of the ectopic expression of keratan sulfate (KS) glycan chains in human malignant melanomas. Using a human melanoma cell line, we found that the KS enhanced the invasiveness of the cells but caused no change in the growth rate of the cells. The phosphorylation of paxillin, a focal adhesion-associated adaptor protein, was strong at the region where KS was expressed in the melanoma tissues, indicating that KS stimulated the phosphorylation of paxillin. We also observed that KS enhanced the adhesion of melanoma cells and this was accompanied by a greatly increased level of phosphorylation of paxillin. These data suggest that the expression of KS contributes to the development of malignant phenotypes such as strong cell adhesion and the invasiveness of melanoma cells.
Identifiants
pubmed: 35811379
doi: 10.1111/1346-8138.16506
doi:
Substances chimiques
Glycolipids
0
Paxillin
0
Proteoglycans
0
Keratan Sulfate
9056-36-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1027-1036Subventions
Organisme : An original foundation to facilitate intra-center collaboration at Osaka International Cancer Institute
Organisme : Grants-in-Aid for Scientific Research C
ID : 20K07629
Organisme : Takeda Science Foundation
Informations de copyright
© 2022 Japanese Dermatological Association.
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