Bile Collected From the Normal Gallbladder of Patients During Surgery Has Simple Bacterial Flora.
bile
gallbladder
human
microbiome
rrna genes
Journal
Cureus
ISSN: 2168-8184
Titre abrégé: Cureus
Pays: United States
ID NLM: 101596737
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
accepted:
05
06
2022
entrez:
11
7
2022
pubmed:
12
7
2022
medline:
12
7
2022
Statut:
epublish
Résumé
Background Bile inhibits bacterial growth because it is rich in bacteriostatic compounds such as bile acids. Analytical techniques using a high-intensity sequencer recently revealed bacterial flora in the bile of normal gallbladders in brain-dead patients. Therefore, we performed a microbial flora analysis of bile collected from pathologically normal gallbladders surgically removed from patients with hepatobiliary pancreatic diseases and normal liver function. Methods Bacterial DNA was extracted from bile samples and analyzed using 16S rRNA sequencing. Results The culture results of all 12 bile samples were negative. However, the results of the 16S ribosome gene analysis suggested the presence of bacterial flora in all samples. The phyla
Identifiants
pubmed: 35812645
doi: 10.7759/cureus.25681
pmc: PMC9257430
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e25681Informations de copyright
Copyright © 2022, Watanabe et al.
Déclaration de conflit d'intérêts
The authors have declared financial relationships, which are detailed in the next section.
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