The First Genomic Analysis of Visna/Maedi Virus Isolates in China.
Illumina sequencing
Maedi-Visna virus
cloning
phylogenetic analysis
provirus
Journal
Frontiers in veterinary science
ISSN: 2297-1769
Titre abrégé: Front Vet Sci
Pays: Switzerland
ID NLM: 101666658
Informations de publication
Date de publication:
2022
2022
Historique:
received:
31
12
2021
accepted:
17
05
2022
entrez:
11
7
2022
pubmed:
12
7
2022
medline:
12
7
2022
Statut:
epublish
Résumé
Visna/Maedi virus (VMV) is a neglected pathogen that damages sheep and goats' nervous and respiratory systems. The virus was discovered 80 years ago and has been endemic in China for nearly four decades; nevertheless, there is little information regarding Chinese isolates' genotypes and genomic characteristics. In this study, the proviral DNA of strains isolated in 1985 and 1994 were extracted, and the proviral DNA was subjected to Illumina sequencing combined with Sanger sequencing of poor coverage regions. The results showed that the two isolates were clustered with genotype A2 and shared 78.3%-89.1% similarity to reference VMV genome sequences, with the highest similarity (88.7%-89.1%) to the USA strain USMARC-200212120-r (accession no. MT993908.1) and lowest similarity (78.3%-78.5%) to the Italian strain SRLV009 (accession no. MG554409.1). A maximum-likelihood tree showed that the Chinese VMV strains and the USA strain 1150 (accession no. MH916859.1) comprise a monophyletic group with a short tree branch. Our data filled the gap in genomic analysis and viral evolution in Chinese VMV strains, and would be benefit China's source-tracing and eradication program development in China.
Identifiants
pubmed: 35812856
doi: 10.3389/fvets.2022.846634
pmc: PMC9263623
doi:
Types de publication
Journal Article
Langues
eng
Pagination
846634Informations de copyright
Copyright © 2022 Wu, Mi, Yang, Wei, Meng, Bolati and Wei.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Viruses. 2018 Dec 08;10(12):
pubmed: 30544780
Gigascience. 2012 Dec 27;1(1):18
pubmed: 23587118
Animals (Basel). 2020 Apr 03;10(4):
pubmed: 32260101
Virus Res. 2010 Aug;151(2):177-84
pubmed: 20466024
PLoS One. 2014 Sep 26;9(9):e108277
pubmed: 25259891
Prev Vet Med. 2020 Mar;176:104905
pubmed: 32004826
Sci Rep. 2020 Feb 10;10(1):2243
pubmed: 32042070
Bioinformatics. 2012 Jun 15;28(12):1647-9
pubmed: 22543367
Vet Clin North Am Food Anim Pract. 2021 Mar;37(1):199-208
pubmed: 33541699
Viruses. 2021 Aug 27;13(9):
pubmed: 34578292
J Gen Virol. 2008 Jun;89(Pt 6):1478-1484
pubmed: 18474564
J Mol Evol. 1980 Dec;16(2):111-20
pubmed: 7463489
Infect Genet Evol. 2010 Oct;10(7):998-1007
pubmed: 20601169
Vet Res. 2016 Jan 06;47:1
pubmed: 26738942
Arch Virol. 2015 Apr;160(4):969-78
pubmed: 25655265
Comp Immunol Microbiol Infect Dis. 2004 Jan;27(1):1-5
pubmed: 14656537
Virology. 2004 Feb 5;319(1):12-26
pubmed: 14967484
Nucleic Acids Res. 2002 Jul 15;30(14):3059-66
pubmed: 12136088
Animals (Basel). 2021 Mar 11;11(3):
pubmed: 33799908
Vet Microbiol. 2010 Jul 29;144(1-2):24-31
pubmed: 20060658
Prev Vet Med. 2020 Mar;176:104886
pubmed: 31986356
Pathogens. 2020 Mar 03;9(3):
pubmed: 32138297
PLoS One. 2020 Nov 18;15(11):e0239916
pubmed: 33206648
Viruses. 2020 Jan 16;12(1):
pubmed: 31963174
Arch Virol. 2008;153(8):1581-5
pubmed: 18584116
J Vet Diagn Invest. 2012 Mar;24(2):392-6
pubmed: 22379056
Vet Res. 2015 Mar 05;46:22
pubmed: 25756342
Vet Pathol. 2017 May;54(3):353-354
pubmed: 28438114
Vet Pathol. 2017 May;54(3):413-424
pubmed: 28113037
Nature. 2016 Dec 22;540(7634):539-543
pubmed: 27880757
PLoS One. 2020 Sep 10;15(9):e0238781
pubmed: 32911525
Vet Microbiol. 2009 Mar 30;135(3-4):231-8
pubmed: 18986775
Vet Med (Auckl). 2018 May 21;9:11-21
pubmed: 30050863