MicroRNA-574 Impacts Granulosa Cell Estradiol Production
ERK1/2 phosphorylation
Estradiol
Granulosa cell
Ovary
TIMP3
miR-574
microRNA
Journal
Frontiers in endocrinology
ISSN: 1664-2392
Titre abrégé: Front Endocrinol (Lausanne)
Pays: Switzerland
ID NLM: 101555782
Informations de publication
Date de publication:
2022
2022
Historique:
received:
10
01
2022
accepted:
12
05
2022
entrez:
11
7
2022
pubmed:
12
7
2022
medline:
14
7
2022
Statut:
epublish
Résumé
Estradiol represents a key steroid ovarian hormone that not only plays a vital role in ovarian follicular development but also is associated with many other reproductive functions. Our primary study revealed that miR-574 expression decreased in porcine granulosa cells during development from small to large follicles, and the increase of ERK1/2 phosphorylation accompanies this change. Since it has been well established that the ERK1/2 activity is tightly associated with granulosa cell functions, including ovarian hormone production, we thus further investigate if the miRNA is involved in the regulation of estradiol production in granulosa cells. We found that overexpression of miR-574 decreased phosphorylated ERK1/2 without affecting the level of ERK1/2 protein, and on the other hand, the inhibition of miR-574 increased phosphorylated ERK1/2 level (P<0.05); meanwhile, overexpression of miR-574 increased estradiol production but knockdown of miR-574 decreased estradiol level in granulosa cells. To further identify the potential mechanism involved in the miR-574 regulatory effect,
Identifiants
pubmed: 35813635
doi: 10.3389/fendo.2022.852127
pmc: PMC9261285
doi:
Substances chimiques
MicroRNAs
0
Tissue Inhibitor of Metalloproteinase-3
0
Estradiol
4TI98Z838E
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
852127Informations de copyright
Copyright © 2022 Pan, Zhan and Li.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Reproduction. 2014 Sep;148(3):271-83
pubmed: 24920665
J Clin Endocrinol Metab. 2016 Apr;101(4):1579-89
pubmed: 26771704
Endocrinology. 2007 Sep;148(9):4292-301
pubmed: 17540724
Genome Biol. 2011 Nov 11;12(11):233
pubmed: 22078297
Endocrinology. 2011 Oct;152(10):3941-51
pubmed: 21846797
Science. 2009 May 15;324(5929):938-41
pubmed: 19443782
Int J Oncol. 2012 Sep;41(3):1043-9
pubmed: 22735305
BMC Genomics. 2014 Jan 14;15:24
pubmed: 24422759
Am J Physiol Cell Physiol. 2018 Mar 1;314(3):C268-C277
pubmed: 29141921
In Vitro Cell Dev Biol Anim. 2016 Mar;52(3):365-373
pubmed: 26676955
Cell Death Dis. 2020 Sep 10;11(9):737
pubmed: 32913213
Dev Cell. 2001 Aug;1(2):239-50
pubmed: 11702783
J Biol Chem. 2016 Jun 3;291(23):12145-60
pubmed: 27080258
Reprod Fertil Dev. 2016 Feb 11;:
pubmed: 26863388
Proc Natl Acad Sci U S A. 2014 Nov 25;111(47):16778-83
pubmed: 25385589
Sci Rep. 2019 Apr 2;9(1):5462
pubmed: 30940861
PLoS One. 2012;7(6):e38640
pubmed: 22737216
Mol Cell Endocrinol. 2018 Dec 5;477:29-38
pubmed: 29775626
Fertil Steril. 2015 Oct;104(4):1037-1046.e1
pubmed: 26209829
Biol Reprod. 2000 Sep;63(3):905-12
pubmed: 10952938
Matrix Biol. 2015 May-Jul;44-46:247-54
pubmed: 25805621
Mol Cell Endocrinol. 2000 May 25;163(1-2):109-16
pubmed: 10963882
Endocrinology. 1991 Sep;129(3):1452-62
pubmed: 1651851
Eur Rev Med Pharmacol Sci. 2019 Aug;23(16):6839-6848
pubmed: 31486483
Biol Reprod. 2001 Sep;65(3):855-65
pubmed: 11514351
Mol Cell Endocrinol. 2015 Jan 5;399:95-102
pubmed: 25150622
Biol Reprod. 2013 Nov 21;89(5):121
pubmed: 24048576
J Biol Chem. 2000 Oct 6;275(40):31226-32
pubmed: 10900194
Front Endocrinol (Lausanne). 2018 Jan 22;8:378
pubmed: 29403434
Mol Endocrinol. 2012 Jul;26(7):1129-43
pubmed: 22593182
Sci Rep. 2019 Nov 8;9(1):16306
pubmed: 31705013
Mol Reprod Dev. 2014 Feb;81(2):148-59
pubmed: 24167089
Biochem Biophys Res Commun. 2001 Dec 14;289(4):796-800
pubmed: 11735115
Eur J Obstet Gynecol Reprod Biol. 2004 Jul 1;115 Suppl 1:S19-22
pubmed: 15196711
J Clin Endocrinol Metab. 2013 Jul;98(7):3068-79
pubmed: 23666971
Biol Reprod. 2001 Sep;65(3):680-8
pubmed: 11514328
Endocrinology. 2001 May;142(5):2058-69
pubmed: 11316773
Endocrinology. 2009 Aug;150(8):3903-12
pubmed: 19389837
Am J Physiol Endocrinol Metab. 2015 Mar 15;308(6):E525-34
pubmed: 25628423
PLoS One. 2015 Mar 16;10(3):e0119800
pubmed: 25775029
J Ovarian Res. 2021 Apr 20;14(1):54
pubmed: 33879202
Blood. 2006 Dec 1;108(12):3646-53
pubmed: 16882713