Graphene Substrates Promote the Differentiation of Inner Ear Lgr5+ Progenitor Cells Into Hair Cells.

differentiation graphene hair cell regeneration proliferation sensorineural hearing loss

Journal

Frontiers in bioengineering and biotechnology
ISSN: 2296-4185
Titre abrégé: Front Bioeng Biotechnol
Pays: Switzerland
ID NLM: 101632513

Informations de publication

Date de publication:
2022
Historique:
received: 24 04 2022
accepted: 03 06 2022
entrez: 11 7 2022
pubmed: 12 7 2022
medline: 12 7 2022
Statut: epublish

Résumé

The ideal treatment for sensory hearing loss is to regenerate inner ear hair cells (HCs) through stem cell therapy, thereby restoring the function and structure of the cochlea. Previous studies have found that Lgr5+ supporting cells (SCs) in the inner ear can regenerate HCs, thus being considered inner ear progenitor cells. In addition to traditional biochemical factors, physical factors such as electrical conductivity also play a crucial role in the regulation of stem cell proliferation and differentiation. In this study, the graphene substrates were used to culture Lgr5+ progenitor cells and investigated their regulatory effects on cells. It was demonstrated that the graphene substrates displayed great cytocompatibility for Lgr5+ progenitors and promoted their sphere-forming ability. Moreover, more Myosin7a+ cells were found on the graphene substrates compared with tissue culture polystyrene (TCPS). These results suggest that graphene is an efficient interface that can promote the differentiation of Lgr5+ progenitors into HCs, which is great significance for its future application in combination with Lgr5+ cells to regenerate HCs in the inner ear.

Identifiants

DOI: 10.3389/fbioe.2022.927248 PMID: 35814013 PMC: PMC9256972
pubmed: 35814013
doi: 10.3389/fbioe.2022.927248
pii: 927248
pmc: PMC9256972
doi:

Types de publication

Journal Article

Langues

eng

Pagination

927248

Informations de copyright

Copyright © 2022 Ding, Hu, Cheng, Zhang, Lu, Gao, Cheng, Wang, Qian, Zhang, Chai, Gao and Huang.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Xiaoqiong Ding (X)

Department of Otorhinolaryngology-Head and Neck Surgery, Nanjing Drum Tower Hospital, Drum Tower Clinical College of Nanjing Medical University, Nanjing, China.
Department of Otorhinolaryngology-Head and Neck Surgery, Zhongda Hospital, Southeast University, Nanjing, China.

Yangnan Hu (Y)

State Key Laboratory of Bioelectronics, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, School of Life Sciences and Technology, Advanced Institute for Life and Health, Southeast University, Nanjing, China.

Hong Cheng (H)

State Key Laboratory of Bioelectronics, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, School of Life Sciences and Technology, Advanced Institute for Life and Health, Southeast University, Nanjing, China.

Xiaoli Zhang (X)

Department of Otorhinolaryngology-Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.

Ling Lu (L)

Department of Otorhinolaryngology-Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.

Song Gao (S)

Department of Otorhinolaryngology-Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.

Cheng Cheng (C)

Department of Otorhinolaryngology-Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.

Lifen Wang (L)

Department of Otorhinolaryngology-Head and Neck Surgery, Zhongda Hospital, Southeast University, Nanjing, China.

Xiaoyun Qian (X)

Department of Otorhinolaryngology-Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.

Chen Zhang (C)

Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing, China.

Renjie Chai (R)

Department of Otorhinolaryngology-Head and Neck Surgery, Zhongda Hospital, Southeast University, Nanjing, China.
State Key Laboratory of Bioelectronics, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, School of Life Sciences and Technology, Advanced Institute for Life and Health, Southeast University, Nanjing, China.
Department of Otorhinolaryngology-Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.
Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing, China.
Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.
Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, China.
Institute for Stem Cell and Regeneration, Chinese Academy of Science, Beijing, China.

Xia Gao (X)

Department of Otorhinolaryngology-Head and Neck Surgery, Nanjing Drum Tower Hospital, Drum Tower Clinical College of Nanjing Medical University, Nanjing, China.

Zhichun Huang (Z)

Department of Otorhinolaryngology-Head and Neck Surgery, Zhongda Hospital, Southeast University, Nanjing, China.

Classifications MeSH