Human neural progenitors establish a diffusion barrier in the endoplasmic reticulum membrane during cell division.
Cell division
Diffusion barrier
ER membrane
Human neural progenitor
Neurogenesis
Organoid
Journal
Development (Cambridge, England)
ISSN: 1477-9129
Titre abrégé: Development
Pays: England
ID NLM: 8701744
Informations de publication
Date de publication:
15 10 2022
15 10 2022
Historique:
received:
02
02
2022
accepted:
20
06
2022
pubmed:
12
7
2022
medline:
9
8
2022
entrez:
11
7
2022
Statut:
ppublish
Résumé
Asymmetric segregation of cellular components regulates the fate and behavior of somatic stem cells. Similar to dividing budding yeast and precursor cells in Caenorhabditis elegans, it has been shown that mouse neural progenitors establish a diffusion barrier in the membrane of the endoplasmic reticulum (ER), which has been associated with asymmetric partitioning of damaged proteins and cellular age. However, the existence of an ER diffusion barrier in human cells remains unknown. Here, we used fluorescence loss in photobleaching (FLIP) imaging to show that human embryonic stem cell (hESC)- and induced pluripotent stem cell (iPSC)-derived neural progenitor cells establish an ER diffusion barrier during cell division. The human ER diffusion barrier is regulated via lamin-dependent mechanisms and is associated with asymmetric segregation of mono- and polyubiquitylated damaged proteins. Further, forebrain regionalized organoids derived from hESCs were used to show the establishment of an ER membrane diffusion barrier in more naturalistic tissues, mimicking early steps of human brain development. Thus, the data provided here show that human neural progenitors establish a diffusion barrier during cell division in the membrane of the ER, which may allow for asymmetric segregation of cellular components, contributing to the fate and behavior of human neural progenitor cells.
Identifiants
pubmed: 35815653
pii: 275957
doi: 10.1242/dev.200613
pmc: PMC9440750
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2022. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing or financial interests.
Références
Curr Opin Cell Biol. 2008 Dec;20(6):723-8
pubmed: 18848886
Cell Stem Cell. 2021 May 6;28(5):967-977.e8
pubmed: 33631115
Cell Stem Cell. 2015 Aug 6;17(2):233-44
pubmed: 26145478
Nat Neurosci. 2002 Dec;5(12):1265-9
pubmed: 12447381
Annu Rev Biochem. 2012;81:203-29
pubmed: 22524316
Trends Cell Biol. 2017 Jan;27(1):82-92
pubmed: 27717533
Cell Stem Cell. 2013 Dec 5;13(6):691-705
pubmed: 24315443
Nat Protoc. 2018 Mar;13(3):565-580
pubmed: 29470464
Elife. 2015 Nov 06;4:
pubmed: 26544680
Aging Cell. 2011 Oct;10(5):885-95
pubmed: 21726403
Nature. 2008 Aug 7;454(7205):728-34
pubmed: 18660802
Cell Stem Cell. 2021 Nov 4;28(11):2020-2034.e12
pubmed: 34525348
Cell. 1994 Feb 11;76(3):477-91
pubmed: 8313469
J Cell Biol. 2005 Jun 20;169(6):897-908
pubmed: 15967812
Nature. 2009 Oct 15;461(7266):947-55
pubmed: 19829375
Cell Rep. 2016 Apr 5;15(1):86-95
pubmed: 27052171
Elife. 2014 May 06;3:e01883
pubmed: 24843009
Sci Adv. 2020 Nov 11;6(46):
pubmed: 33177076
Science. 2015 Apr 17;348(6232):340-3
pubmed: 25837514
Cell. 2016 May 19;165(5):1238-1254
pubmed: 27118425
J Cell Sci. 2019 Jun 24;132(12):
pubmed: 31164445
Science. 2015 Sep 18;349(6254):1334-8
pubmed: 26383951
Nat Rev Mol Cell Biol. 2010 Dec;11(12):849-60
pubmed: 21102610
Nature. 2019 Sep;573(7774):426-429
pubmed: 31485073
J Cell Biol. 2016 Sep 12;214(6):665-76
pubmed: 27597753