Visible Light Bioluminescence Imaging Platform for Animal Cell Imaging.
Bioluminescence (BL)
Coelenterazine
Imaging
Luciferase
Redshifts
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
13
7
2022
pubmed:
14
7
2022
medline:
15
7
2022
Statut:
ppublish
Résumé
The present protocol introduces a visible light bioluminescence imaging (BLI) platform together with 12 novel coelenterazine (CTZ) analogues and luciferase sets. We exemplify to create diverse hues of bioluminescence (BL) ranging from blue to far red with the combination of marine luciferases and the three groups of CTZ analogues. We also show how to characterize the new CTZ analogues in detail such as the kinetic parameters, dose dependency, and luciferase specificity. The 2-series CTZ analogues interestingly have specificity to artificial luciferases and are completely dark with Renilla luciferase derivatives in contrast. The 3d is highly specific to only NanoLuc. This BL imaging system covering the visible region provides a useful multicolor imaging portfolio that efficiently images molecular events in mammalian cells.
Identifiants
pubmed: 35821461
doi: 10.1007/978-1-0716-2453-1_3
doi:
Substances chimiques
Luciferases
EC 1.13.12.-
nanoluc
EC 1.13.12.-
Luciferases, Renilla
EC 1.13.12.5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
37-51Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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