Highly Efficient Purification of Recombinant VSV-∆G-Spike Vaccine against SARS-CoV-2 by Flow-Through Chromatography.
SARS-CoV-2
chromatography
downstream process
membrane adsorbers
rVSV
Journal
Biotech (Basel (Switzerland))
ISSN: 2673-6284
Titre abrégé: BioTech (Basel)
Pays: Switzerland
ID NLM: 9918383086206676
Informations de publication
Date de publication:
12 Oct 2021
12 Oct 2021
Historique:
received:
14
09
2021
revised:
07
10
2021
accepted:
08
10
2021
entrez:
13
7
2022
pubmed:
14
7
2022
medline:
14
7
2022
Statut:
epublish
Résumé
This study reports a highly efficient, rapid one-step purification process for the production of the recombinant vesicular stomatitis virus-based vaccine, rVSV-∆G-spike (rVSV-S), recently developed by the Israel Institute for Biological Research (IIBR) for the prevention of COVID-19. Several purification strategies are evaluated using a variety of chromatography methods, including membrane adsorbers and packed-bed ion-exchange chromatography. Cell harvest is initially treated with endonuclease, clarified, and further concentrated by ultrafiltration before chromatography purification. The use of anion-exchange chromatography in all forms results in strong binding of the virus to the media, necessitating a high salt concentration for elution. The large virus and spike protein binds very strongly to the high surface area of the membrane adsorbents, resulting in poor virus recovery (<15%), while the use of packed-bed chromatography, where the surface area is smaller, achieves better recovery (up to 33%). Finally, a highly efficient chromatography purification process with Capto
Identifiants
pubmed: 35822796
pii: biotech10040022
doi: 10.3390/biotech10040022
pmc: PMC9245476
pii:
doi:
Types de publication
Journal Article
Langues
eng
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