Intracellular Absolute Quantification of Oligonucleotide Therapeutics by NanoSIMS.
Journal
Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536
Informations de publication
Date de publication:
26 07 2022
26 07 2022
Historique:
pubmed:
14
7
2022
medline:
28
7
2022
entrez:
13
7
2022
Statut:
ppublish
Résumé
Antisense oligonucleotide (ASO)-based therapeutics hold great potential for the treatment of a variety of diseases. Therefore, a better understanding of cellular delivery, uptake, and trafficking mechanisms of ASOs is highly important for early-stage drug discovery. In particular, understanding the biodistribution and quantifying the abundance of ASOs at the subcellular level are needed to fully characterize their activity. Here, we used a combination of electron microscopy and NanoSIMS to assess the subcellular concentrations of a
Identifiants
pubmed: 35830231
doi: 10.1021/acs.analchem.2c02111
doi:
Substances chimiques
Oligonucleotides
0
Oligonucleotides, Antisense
0
Iodine
9679TC07X4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM