A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution.

Animals Bone Marrow Immunity, Innate Lymphocytes Lymphoid Progenitor Cells Mice T-Lymphocytes
Cell Biology Cell isolation Flow Cytometry/Mass Cytometry Immunology

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
16 09 2022
Historique:
received: 23 03 2022
revised: 20 05 2022
accepted: 15 06 2022
pubmed: 14 7 2022
medline: 23 9 2022
entrez: 13 7 2022
Statut: ppublish

Résumé

Innate lymphoid cells (ILCs) and adaptive T cells remain a challenge to study because of a significant overlap in their transcriptomic profiles. Here, we describe the adoptive transfer of ILC progenitors into mice genetically deficient in innate and adaptive immune cells to allow detailed study of the development and function of ILCs and gene regulation in an in vivo setting. For complete details on the use and execution of this protocol, please refer to Jacquelot et al. (2021) and Seillet et al. (2016).

Identifiants

DOI: 10.1016/j.xpro.2022.101534 PMID: 35830307 PMC: PMC9287798
pubmed: 35830307
pii: S2666-1667(22)00414-2
doi: 10.1016/j.xpro.2022.101534
pmc: PMC9287798
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101534

Informations de copyright

Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Auteurs

Nicolas Jacquelot (N)

Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2M9, Canada. Electronic address: nicolas.jacquelot@uhnresearch.ca.

Qiutong Huang (Q)

The University of Queensland Diamantina Institute, Faculty of Medicine, The University of Queensland, Woolloongabba, QLD 4102, Australia.

Gabrielle T Belz (GT)

The University of Queensland Diamantina Institute, Faculty of Medicine, The University of Queensland, Woolloongabba, QLD 4102, Australia; Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 3010, Australia. Electronic address: g.belz@uq.edu.au.

Cyril Seillet (C)

Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 3010, Australia. Electronic address: seillet@wehi.edu.au.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › A protocol to isolate bone marrow innate...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Moelle osseuse › A protocol to isolate bone marrow innate...
questionsmedicales.fr › Phénomènes du système immunitaire › Immunité › Immunité innée › A protocol to isolate bone marrow innate...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › A protocol to isolate bone marrow innate...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système hématopoïétique › Cellules de la moelle osseuse › Cellules souches hématopoïétiques › Progéniteurs lymphoïdes › A protocol to isolate bone marrow innate...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › A protocol to isolate bone marrow innate...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Lymphocytes T › A protocol to isolate bone marrow innate...