Isotropic imaging across spatial scales with axially swept light-sheet microscopy.
Journal
Nature protocols
ISSN: 1750-2799
Titre abrégé: Nat Protoc
Pays: England
ID NLM: 101284307
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
received:
21
07
2021
accepted:
30
03
2022
pubmed:
14
7
2022
medline:
9
9
2022
entrez:
13
7
2022
Statut:
ppublish
Résumé
Light-sheet fluorescence microscopy is a rapidly growing technique that has gained tremendous popularity in the life sciences owing to its high-spatiotemporal resolution and gentle, non-phototoxic illumination. In this protocol, we provide detailed directions for the assembly and operation of a versatile light-sheet fluorescence microscopy variant, referred to as axially swept light-sheet microscopy (ASLM), that delivers an unparalleled combination of field of view, optical resolution and optical sectioning. To democratize ASLM, we provide an overview of its working principle and applications to biological imaging, as well as pragmatic tips for the assembly, alignment and control of its optical systems. Furthermore, we provide detailed part lists and schematics for several variants of ASLM that together can resolve molecular detail in chemically expanded samples, subcellular organization in living cells or the anatomical composition of chemically cleared intact organisms. We also provide software for instrument control and discuss how users can tune imaging parameters to accommodate diverse sample types. Thus, this protocol will serve not only as a guide for both introductory and advanced users adopting ASLM, but as a useful resource for any individual interested in deploying custom imaging technology. We expect that building an ASLM will take ~1-2 months, depending on the experience of the instrument builder and the version of the instrument.
Identifiants
pubmed: 35831614
doi: 10.1038/s41596-022-00706-6
pii: 10.1038/s41596-022-00706-6
pmc: PMC10111370
mid: NIHMS1883634
doi:
Types de publication
Journal Article
Review
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2025-2053Subventions
Organisme : NIMH NIH HHS
ID : R01 MH120131
Pays : United States
Organisme : NICHD NIH HHS
ID : R21 HD105189
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM133522
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK127589
Pays : United States
Organisme : NCI NIH HHS
ID : R33 CA235254
Pays : United States
Organisme : NIBIB NIH HHS
ID : T32 EB019941
Pays : United States
Informations de copyright
© 2022. Springer Nature Limited.
Références
Nat Methods. 2017 Mar 31;14(4):360-373
pubmed: 28362435
Nat Methods. 2011 May;8(5):417-23
pubmed: 21378978
Opt Express. 2014 Oct 20;22(21):26141-52
pubmed: 25401646
J Biomed Opt. 2020 Oct;25(10):
pubmed: 33098281
Biol Bull. 2016 Aug;231(1):14-25
pubmed: 27638692
Biomed Opt Express. 2017 Sep 07;8(10):4369-4379
pubmed: 29082071
Nat Methods. 2021 Jun;18(6):582-584
pubmed: 34002092
J Biol Methods. 2014;1(2):
pubmed: 25606571
Nat Methods. 2014 Jun;11(6):645-8
pubmed: 24747812
Cell. 2014 Apr 24;157(3):726-39
pubmed: 24746791
Cell Res. 2018 Aug;28(8):803-818
pubmed: 29844583
Nat Methods. 2019 Nov;16(11):1109-1113
pubmed: 31673159
Opt Express. 2020 Aug 31;28(18):27052-27077
pubmed: 32906967
Science. 2015 Jan 30;347(6221):543-8
pubmed: 25592419
Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):2919-24
pubmed: 22315405
J Microsc. 1993 Jun;170(Pt 3):229-36
pubmed: 8371260
Opt Lett. 2007 Sep 1;32(17):2608-10
pubmed: 17767321
Biophys J. 2015 Jun 16;108(12):2807-15
pubmed: 26083920
Microsc Res Tech. 2018 Sep;81(9):924-928
pubmed: 27338568
Opt Express. 2007 Jun 25;15(13):8029-42
pubmed: 19547131
Opt Express. 2013 Sep 9;21(18):21010-26
pubmed: 24103973
Light Sci Appl. 2020 Sep 18;9:165
pubmed: 33024553
Opt Express. 2015 Mar 9;23(5):6102-11
pubmed: 25836834
Nat Protoc. 2014 May;9(5):1083-101
pubmed: 24722406
Dev Cell. 2019 May 6;49(3):444-460.e9
pubmed: 31063759
Nature. 2013 May 16;497(7449):332-7
pubmed: 23575631
Science. 2014 Oct 24;346(6208):1257998
pubmed: 25342811
Opt Lett. 2007 Jul 15;32(14):2007-9
pubmed: 17632625
Nat Methods. 2007 Apr;4(4):331-6
pubmed: 17384643
Dev Cell. 2020 Dec 21;55(6):723-736.e8
pubmed: 33308479
Biotechniques. 2009 Apr;46(4):287-94
pubmed: 19450235
Am J Pathol. 2003 Sep;163(3):1045-56
pubmed: 12937145
Biomed Opt Express. 2019 Dec 02;11(1):8-26
pubmed: 32010496
Opt Express. 2013 May 6;21(9):11425-40
pubmed: 23669999
Nat Biotechnol. 2013 Nov;31(11):1032-8
pubmed: 24108093
Nat Methods. 2014 May;11(5):541-4
pubmed: 24705473
Nat Commun. 2020 Jul 31;11(1):3850
pubmed: 32737322
Nat Methods. 2019 Mar;16(3):235-238
pubmed: 30804550
Elife. 2016 Jun 14;5:
pubmed: 27300105
Nat Methods. 2015 Dec;12(12):1171-8
pubmed: 26501515
Science. 2004 Aug 13;305(5686):1007-9
pubmed: 15310904
Opt Lett. 2018 Nov 1;43(21):5484-5487
pubmed: 30383037
Nat Methods. 2021 Mar;18(3):226-228
pubmed: 33674797
Dev Cell. 2016 Feb 22;36(4):462-75
pubmed: 26906741
Nat Methods. 2019 Nov;16(11):1105-1108
pubmed: 31527839