Enzyme sensor for simultaneous determination of cholesterol, triglyceride and HDL cholesterol in serum.
Cholesterol
Enzyme sensor
HDL cholesterol
Triglyceride
Journal
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
ISSN: 1348-2246
Titre abrégé: Anal Sci
Pays: Switzerland
ID NLM: 8511078
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
received:
24
03
2022
accepted:
07
06
2022
pubmed:
14
7
2022
medline:
31
8
2022
entrez:
13
7
2022
Statut:
ppublish
Résumé
A three-electrode lipid biosensor that simultaneously measures the total cholesterol (CHO), triglyceride (TG), and HDL cholesterol (HDL-C) in standard serum has been developed. The lipid biosensor is designed for clinical use where production cost, low sample requirement, portability, stability, and speed are high priorities. The device design filters out blood cells and lipoproteins from the serum, where the target molecules are catalyzed by enzymes encapsulated in carboxymethyl cellulose (CMC) to produce electron mediators, potassium ferrocyanide. These electron mediators were subsequently detected by amperometric determination. The sensor exhibit high selectivity towards the targets and can measure the target lipids in 4 min with 10 µL of serum. Lastly, the device can be stored up to 18 months with a minimal decrease in catalytic efficiency.
Identifiants
pubmed: 35831775
doi: 10.1007/s44211-022-00148-w
pii: 10.1007/s44211-022-00148-w
doi:
Substances chimiques
Cholesterol, HDL
0
Lipoproteins
0
Triglycerides
0
Cholesterol
97C5T2UQ7J
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1189-1197Informations de copyright
© 2022. The Author(s), under exclusive licence to The Japan Society for Analytical Chemistry.
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