Full Assessment of Lung Mechanics Using Computer-Controlled, Forced Oscillation Technique.
FlexiVentFX
endotracheal intubation
forced oscillation technique
lung function
lung mechanics
Journal
Current protocols
ISSN: 2691-1299
Titre abrégé: Curr Protoc
Pays: United States
ID NLM: 101773894
Informations de publication
Date de publication:
Jul 2022
Jul 2022
Historique:
entrez:
14
7
2022
pubmed:
15
7
2022
medline:
19
7
2022
Statut:
ppublish
Résumé
The forced oscillation technique (FOT) is a powerful and accurate method to quantify the mechanical properties of the airways and tissues of the respiratory system. Here we provide a detailed protocol for the measurement of mouse respiratory mechanical parameters. We present a procedure for mouse endotracheal intubation using a handcrafted intubation platform and confirmation module. The FlexiVentFX™ system (Scireq Inc.) is utilized for the thorough assessment of lung function with the FlexiWare™ software serving as a unit for the planning, experimentation, and analysis. The protocol has been standardized and adapted for use by our center for lung-function phenotyping of mouse models generated for the International Mouse Phenotyping Consortium (IMPC). The simplified steps, technical considerations, and integrated hardware-software demonstration make this protocol adaptable and implementable for researchers interested in using FOT for lung-function evaluation. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC. Support Protocol 1: Assembly of the FlexiVentFX™ system for measurements Support Protocol 2: FlexiWare database management Support Protocol 3: A guide for the construction of intubation platform and confirmation module Basic Protocol 1: Mouse endotracheal intubation Basic Protocol 2: Assessment of mouse basal lung function.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e488Subventions
Organisme : Czech Academy of Sciences
ID : RVO 68378050
Organisme : Ministry of Education, Youth, and Sports of the Czech Republic
Informations de copyright
© 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.
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