Dynamic behavior of cell-free mitochondrial DNA in human saliva.
Awakening response
Cell-free mitochondrial DNA
Saliva
Steroid hormones
Journal
Psychoneuroendocrinology
ISSN: 1873-3360
Titre abrégé: Psychoneuroendocrinology
Pays: England
ID NLM: 7612148
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
received:
28
12
2021
revised:
20
05
2022
accepted:
23
06
2022
pubmed:
15
7
2022
medline:
4
8
2022
entrez:
14
7
2022
Statut:
ppublish
Résumé
Mitochondria contain their own genome that can be released in multiple biofluids such as blood and cerebrospinal fluid, as cell-free mitochondrial DNA (cf-mtDNA). In clinical studies, blood cf-mtDNA predicts mortality and higher cf-mtDNA levels are associated with mental and physical stress. However, the dynamics of cf-mtDNA has not been defined, and whether it can be measured non-invasively like other neuroendocrine markers in saliva has not been examined. Here we report cf-mtDNA in human saliva and establish its natural within-person dynamic behavior across multiple weeks. In a small proof-of-principle cohort of healthy adults, we first develop an approach to rapidly quantify salivary cf-mtDNA without DNA isolation, and demonstrate the existence of salivary cf-mtDNA. We then deploy this approach to perform an intensive repeated-measures analysis of two healthy men studied at 4 daily timepoints over 53-60 consecutive days (n = 212-220 observations each) with parallel measures of steroid hormones, self-reported daily mood, and health-related behaviors. Salivary cf-mtDNA exhibited a robust awakening response reaching up to two orders of magnitude 30-45 min after awakening, varied from day-to-day, and moderately correlated with the cortisol awakening response. In exploratory analyses, no consistent association with self-reported daily mood/health-related behaviors were found, although this requires further examination in future studies. Dynamic variation in cf-mtDNA was inversely related with salivary interleukin 6 (IL-6), inconsistent with a pro-inflammatory effect of salivary cf-mtDNA. The highly dynamic behavior of salivary cf-mtDNA opens the door to non-invasive studies examining the relevance of mtDNA signaling in relation to human health.
Identifiants
pubmed: 35834882
pii: S0306-4530(22)00193-7
doi: 10.1016/j.psyneuen.2022.105852
pmc: PMC9880596
mid: NIHMS1864499
pii:
doi:
Substances chimiques
Cell-Free Nucleic Acids
0
DNA, Mitochondrial
0
Hydrocortisone
WI4X0X7BPJ
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
105852Subventions
Organisme : NIMH NIH HHS
ID : R01 MH119336
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH122706
Pays : United States
Organisme : NIMH NIH HHS
ID : R21 MH123927
Pays : United States
Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2022 Elsevier Ltd. All rights reserved.
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