Microplastics detected in cirrhotic liver tissue.

Animals Ecosystem Environmental Monitoring Humans Liver Cirrhosis Mice Microplastics Plastics / chemistry Polymers Water Pollutants, Chemical / analysis
Human tissue Liver cirrhosis Microplastics Raman spectroscopy

Journal

EBioMedicine
ISSN: 2352-3964
Titre abrégé: EBioMedicine
Pays: Netherlands
ID NLM: 101647039

Informations de publication

Date de publication:
Aug 2022
Historique:
received: 09 04 2022
revised: 22 06 2022
accepted: 22 06 2022
pubmed: 15 7 2022
medline: 17 8 2022
entrez: 14 7 2022
Statut: ppublish

Résumé

The contamination of ecosystem compartments by microplastics (MPs) is an ubiquitous problem. MPs have been observed in mice tissues, and recently in human blood, stool and placenta. However, two aspects remain unclear: whether MPs accumulate in peripheral organs, specifically in the liver, and if liver cirrhosis favours this process. We aimed to examine human liver tissue samples to determine whether MPs accumulate in the liver. This proof-of-concept case series, conducted in Germany, Europe, analyzed tissue samples of 6 patients with liver cirrhosis and 5 individuals without underlying liver disease. A total of 17 samples (11 liver, 3 kidney and 3 spleen samples) were analyzed according to the final protocol. A reliable method for detection of MP particles from 4 to 30 µm in human tissue was developed. Chemical digestion of tissue samples, staining with Nile red, subsequent fluorescent microscopy and Raman spectroscopy were performed. Morphology, size and composition of MP polymers were assessed. Considering the limit of detection, all liver, kidney and spleen samples from patients without underlying liver disease tested negative for MPs. In contrast, MP concentrations in cirrhotic liver tissues tested positive and showed significantly higher concentrations compared to liver samples of individuals without underlying liver disease. Six different microplastic polymers ranging from 4 to 30 µm in size were detected. This proof-of-concept case series assessed the presence of MPs in human liver tissue and found six different MP polymers in the liver of individuals with liver cirrhosis, but not in those without underlying liver disease. Future studies are needed to evaluate whether hepatic MP accumulation represents a potential cause in the pathogenesis of fibrosis, or a consequence of cirrhosis and portal hypertension. No funding was received for conducting this investigator driven study.

Sections du résumé

BACKGROUND BACKGROUND
The contamination of ecosystem compartments by microplastics (MPs) is an ubiquitous problem. MPs have been observed in mice tissues, and recently in human blood, stool and placenta. However, two aspects remain unclear: whether MPs accumulate in peripheral organs, specifically in the liver, and if liver cirrhosis favours this process. We aimed to examine human liver tissue samples to determine whether MPs accumulate in the liver.
METHODS METHODS
This proof-of-concept case series, conducted in Germany, Europe, analyzed tissue samples of 6 patients with liver cirrhosis and 5 individuals without underlying liver disease. A total of 17 samples (11 liver, 3 kidney and 3 spleen samples) were analyzed according to the final protocol. A reliable method for detection of MP particles from 4 to 30 µm in human tissue was developed. Chemical digestion of tissue samples, staining with Nile red, subsequent fluorescent microscopy and Raman spectroscopy were performed. Morphology, size and composition of MP polymers were assessed.
FINDINGS RESULTS
Considering the limit of detection, all liver, kidney and spleen samples from patients without underlying liver disease tested negative for MPs. In contrast, MP concentrations in cirrhotic liver tissues tested positive and showed significantly higher concentrations compared to liver samples of individuals without underlying liver disease. Six different microplastic polymers ranging from 4 to 30 µm in size were detected.
INTERPRETATION CONCLUSIONS
This proof-of-concept case series assessed the presence of MPs in human liver tissue and found six different MP polymers in the liver of individuals with liver cirrhosis, but not in those without underlying liver disease. Future studies are needed to evaluate whether hepatic MP accumulation represents a potential cause in the pathogenesis of fibrosis, or a consequence of cirrhosis and portal hypertension.
FUNDING BACKGROUND
No funding was received for conducting this investigator driven study.

Identifiants

DOI: 10.1016/j.ebiom.2022.104147 PMID: 35835713 PMC: PMC9386716
pubmed: 35835713
pii: S2352-3964(22)00328-0
doi: 10.1016/j.ebiom.2022.104147
pmc: PMC9386716
pii:
doi:

Substances chimiques

Microplastics 0
Plastics 0
Polymers 0
Water Pollutants, Chemical 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

104147

Informations de copyright

Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests All authors declare that they have no conflict of interests regarding this manuscript.

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Auteurs

Thomas Horvatits (T)

I. Department of Medicine, Gastroenterology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. Electronic address: t.horvatits@uke.de.

Matthias Tamminga (M)

Center for Earth System Research and Sustainability (CEN), University of Hamburg, Hamburg, Germany.

Beibei Liu (B)

I. Department of Medicine, Gastroenterology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Marcial Sebode (M)

I. Department of Medicine, Gastroenterology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Antonella Carambia (A)

I. Department of Medicine, Gastroenterology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Lutz Fischer (L)

Department of Transplant Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Klaus Püschel (K)

Institute of Legal Medicine, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany.

Samuel Huber (S)

I. Department of Medicine, Gastroenterology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Elke Kerstin Fischer (EK)

Center for Earth System Research and Sustainability (CEN), University of Hamburg, Hamburg, Germany. Electronic address: elke.fischer@uni-hamburg.de.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Microplastics detected in cirrhotic liver tissue.
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Microplastics detected in cirrhotic liver tissue.
questionsmedicales.fr › Environnement et santé publique › Santé publique › Pratiques en santé publique › Surveillance de l'environnement › Microplastics detected in cirrhotic liver tissue.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Microplastics detected in cirrhotic liver tissue.
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Fibrose › Cirrhose du foie › Microplastics detected in cirrhotic liver tissue.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Microplastics detected in cirrhotic liver tissue.
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Matières plastiques › Microplastiques › Microplastics detected in cirrhotic liver tissue.
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Matières plastiques › Microplastics detected in cirrhotic liver tissue.
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Microplastics detected in cirrhotic liver tissue.
questionsmedicales.fr › Actions chimiques et utilisations › Actions toxiques › Polluants environnementaux › Polluants de l'eau › Polluants chimiques de l'eau › Microplastics detected in cirrhotic liver tissue.