A multimodal imaging workflow for monitoring CAR T cell therapy against solid tumor from whole-body to single-cell level.
3D µCT/BLT
CAR T Cells
Cell Tracking
Light-Sheet Fluorescence microscopy
Optical tomography
Journal
Theranostics
ISSN: 1838-7640
Titre abrégé: Theranostics
Pays: Australia
ID NLM: 101552395
Informations de publication
Date de publication:
2022
2022
Historique:
received:
11
11
2021
accepted:
19
05
2022
entrez:
15
7
2022
pubmed:
16
7
2022
medline:
19
7
2022
Statut:
epublish
Résumé
CAR T cell research in solid tumors often lacks spatiotemporal information and therefore, there is a need for a molecular tomography to facilitate high-throughput preclinical monitoring of CAR T cells. Furthermore, a gap exists between macro- and microlevel imaging data to better assess intratumor infiltration of therapeutic cells. We addressed this challenge by combining 3D µComputer tomography bioluminescence tomography (µCT/BLT), light-sheet fluorescence microscopy (LSFM) and cyclic immunofluorescence (IF) staining.
Identifiants
pubmed: 35836798
doi: 10.7150/thno.68966
pii: thnov12p4834
pmc: PMC9274742
doi:
Substances chimiques
Interleukin-2
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4834-4850Informations de copyright
© The author(s).
Déclaration de conflit d'intérêts
Competing Interests: R.P., K.W., A.G., C.L., K.B., M.B., A.K., and O.H., are employees of Miltenyi Biotec B.V. & Co. KG. F.G. is founder of Gremse-IT. W.A is an employee of Ossium Health Inc. All other authors declare no competing interests.
Références
Theranostics. 2016 Jan 01;6(3):328-41
pubmed: 26909109
Clin Cancer Res. 2019 Aug 1;25(15):4663-4673
pubmed: 31053602
Nat Commun. 2021 Mar 5;12(1):1453
pubmed: 33674603
Biol Blood Marrow Transplant. 2019 Jun;25(6):1092-1098
pubmed: 30769193
Front Immunol. 2020 Jun 17;11:1109
pubmed: 32625204
Sci Transl Med. 2017 Jul 19;9(399):
pubmed: 28724573
J Immunol. 2013 Sep 1;191(5):2064-71
pubmed: 23898039
Cell Death Dis. 2018 Feb 7;9(2):177
pubmed: 29415996
Trends Cancer. 2018 May;4(5):337-341
pubmed: 29709257
Front Immunol. 2018 May 22;9:1104
pubmed: 29872437
Cancer Invest. 2019;37(9):463-477
pubmed: 31490702
JAMA Oncol. 2017 Aug 01;3(8):1094-1101
pubmed: 28426845
Oncotarget. 2017 May 29;8(37):61425-61439
pubmed: 28977875
Sci Transl Med. 2014 Feb 19;6(224):224ra25
pubmed: 24553386
Cell. 2020 Oct 1;183(1):126-142.e17
pubmed: 32961131
N Engl J Med. 2016 Dec 29;375(26):2561-9
pubmed: 28029927
Sci Rep. 2022 Feb 3;12(1):1911
pubmed: 35115587
J Clin Endocrinol Metab. 2021 Jan 1;106(1):120-132
pubmed: 33000173
Immunopharmacol Immunotoxicol. 2016;38(1):50-60
pubmed: 26473647
Proc Natl Acad Sci U S A. 2019 Jan 29;116(5):1692-1697
pubmed: 30635425
Blood. 2005 Dec 1;106(12):3718-24
pubmed: 16081690
N Engl J Med. 2014 Oct 16;371(16):1507-17
pubmed: 25317870
Mol Ther. 2020 Oct 7;28(10):2271-2285
pubmed: 32645298
Front Immunol. 2018 Jul 27;9:1740
pubmed: 30140266
J Nucl Med. 2018 Dec;59(12):1894-1900
pubmed: 29903928
Cancer Res. 2020 Nov 1;80(21):4731-4740
pubmed: 32958548
J Clin Invest. 2019 Apr 1;129(4):1785-1800
pubmed: 30753167
JCI Insight. 2021 Jan 25;6(2):
pubmed: 33332284
Angiogenesis. 2017 Nov;20(4):409-426
pubmed: 28660302
Theranostics. 2017 Apr 5;7(6):1499-1510
pubmed: 28529633
Front Oncol. 2021 May 28;11:664688
pubmed: 34123825
Nat Commun. 2018 Jul 13;9(1):2724
pubmed: 30006565
Nat Commun. 2021 Sep 20;12(1):5547
pubmed: 34545095
Nat Commun. 2021 Sep 27;12(1):5668
pubmed: 34580291
Mol Ther. 2010 Apr;18(4):843-51
pubmed: 20179677
J Immunol. 2000 Dec 1;165(11):6037-46
pubmed: 11086035
J Immunother. 2019 May;42(4):126-135
pubmed: 30882547
Eur J Nucl Med Mol Imaging. 2022 Jun;49(7):2447-2449
pubmed: 35146579
Sci Transl Med. 2014 Nov 5;6(261):261ra151
pubmed: 25378643
Immunotherapy. 2021 Jan;13(1):5-10
pubmed: 33045890
Nat Med. 2019 Sep;25(9):1408-1414
pubmed: 31477906
Mol Ther. 2020 Jan 8;28(1):42-51
pubmed: 31668558
Sci Transl Med. 2017 Jan 18;9(373):
pubmed: 28100832
J Immunol. 2015 Feb 1;194(3):911-20
pubmed: 25520398
Aging (Albany NY). 2020 Sep 24;12(18):18741-18753
pubmed: 32973124
Sci Rep. 2016 Nov 04;6:36173
pubmed: 27812027
Front Immunol. 2018 Nov 08;9:2593
pubmed: 30467505
J Control Release. 2013 Dec 10;172(2):523-34
pubmed: 23680286
Annu Rev Med. 2017 Jan 14;68:139-152
pubmed: 27860544
Cancer Res. 2019 Jun 15;79(12):3112-3124
pubmed: 31015228
J Immunother Emphasis Tumor Immunol. 1996 Nov;19(6):419-27
pubmed: 9041461
Front Immunol. 2020 Aug 07;11:1704
pubmed: 32849600
Mol Imaging Biol. 2022 Apr;24(2):235-248
pubmed: 34816284
Mol Ther. 2013 Apr;21(4):904-12
pubmed: 23423337
Nat Commun. 2018 Jan 9;9(1):132
pubmed: 29317625
Mol Ther Oncolytics. 2021 Jun 12;22:355-367
pubmed: 34553024
Int J Mol Sci. 2020 May 08;21(9):
pubmed: 32397303
J Clin Invest. 2020 Sep 1;130(9):4652-4662
pubmed: 32437331
J Clin Oncol. 2015 May 20;33(15):1688-96
pubmed: 25800760
Nat Rev Immunol. 2020 Dec;20(12):739-755
pubmed: 32728220
J Clin Oncol. 2006 May 1;24(13):e20-2
pubmed: 16648493
JCI Insight. 2016 Nov 17;1(19):e90064
pubmed: 27882353
Cancer Res. 2015 Sep 1;75(17):3596-607
pubmed: 26330166
Nat Rev Clin Oncol. 2020 Mar;17(3):147-167
pubmed: 31848460
Clin Cancer Res. 2006 Oct 15;12(20 Pt 1):6106-15
pubmed: 17062687
Phys Med Biol. 2014 Jan 6;59(1):R1-64
pubmed: 24334634
Blood. 2016 Jun 30;127(26):3321-30
pubmed: 27207799
Sci Rep. 2017 Oct 30;7(1):14366
pubmed: 29085043
Int J Mol Imaging. 2013;2013:287697
pubmed: 23936647
Gastroenterology. 2018 Nov;155(5):1625-1639.e2
pubmed: 30092175
Bone Marrow Transplant. 2021 May;56(5):1134-1143
pubmed: 33268830
PLoS Pathog. 2015 Oct 20;11(10):e1005177
pubmed: 26485519
Stem Cells Dev. 2009 Jun;18(5):683-92
pubmed: 19099374