Humanized mice for investigating sustained Plasmodium vivax blood-stage infections and transmission.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
15 07 2022
15 07 2022
Historique:
received:
15
09
2021
accepted:
07
07
2022
entrez:
15
7
2022
pubmed:
16
7
2022
medline:
20
7
2022
Statut:
epublish
Résumé
Plasmodium vivax is the most widespread human malaria parasite. Due to the presence of extravascular reservoirs and relapsing infections from dormant liver stages, P. vivax is particularly difficult to control and eliminate. Experimental research is hampered by the inability to maintain P. vivax cultures in vitro, due to its tropism for immature red blood cells (RBCs). Here, we describe a new humanized mice model that can support efficient human erythropoiesis and maintain long-lasting multiplication of inoculated cryopreserved P. vivax parasites and their sexual differentiation, including in bone marrow. Mature gametocytes were transmitted to Anopheles mosquitoes, which led to the formation of salivary gland sporozoites. Importantly, blood-stage P. vivax parasites were maintained after the secondary transfer of fresh or frozen infected bone marrow cells to naïve chimeras. This model provides a unique tool for investigating, in vivo, the biology of intraerythrocytic P. vivax.
Identifiants
pubmed: 35840625
doi: 10.1038/s41467-022-31864-6
pii: 10.1038/s41467-022-31864-6
pmc: PMC9287384
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4123Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2022. The Author(s).
Références
Int J Lab Hematol. 2014 Apr;36(2):184-96
pubmed: 24118926
PLoS Negl Trop Dis. 2017 Apr 6;11(4):e0005365
pubmed: 28384192
Leukemia. 2004 Mar;18(3):575-83
pubmed: 14749701
Blood Adv. 2019 Feb 12;3(3):268-274
pubmed: 30696625
Front Immunol. 2021 Jul 27;12:671648
pubmed: 34386001
Trends Parasitol. 2021 Apr;37(4):273-282
pubmed: 33257270
Ultrastruct Pathol. 2009 Oct;33(5):236-42
pubmed: 19895296
Trends Parasitol. 2020 Jul;36(7):648-649
pubmed: 32448705
PLoS Negl Trop Dis. 2017 Jul 31;11(7):e0005824
pubmed: 28759591
Nat Methods. 2009 Apr;6(4):267-9
pubmed: 19270698
PLoS Biol. 2020 May 4;18(5):e3000711
pubmed: 32365102
Stem Cell Reports. 2016 Oct 11;7(4):591-601
pubmed: 27618723
Blood. 1997 Aug 15;90(4):1345-64
pubmed: 9269751
iScience. 2020 Aug 21;23(8):101381
pubmed: 32739836
J Immunol. 2014 Aug 1;193(3):1504-11
pubmed: 24973440
Trends Parasitol. 2020 Jun;36(6):512-519
pubmed: 32360314
J Lab Clin Med. 1948 Dec;33(12):1579-87
pubmed: 18100165
Stem Cell Reports. 2015 Feb 10;4(2):171-80
pubmed: 25601207
J Med Entomol. 2005 Sep;42(5):777-9
pubmed: 16363160
J Infect Dis. 2013 Sep 1;208(5):801-12
pubmed: 23766527
Blood. 2011 Nov 24;118(22):5938-46
pubmed: 21926352
Science. 2018 Jan 5;359(6371):48-55
pubmed: 29302006
Front Immunol. 2018 Dec 13;9:2550
pubmed: 30631319
J Infect Dis. 2022 May 4;225(9):1621-1625
pubmed: 34453537
Trends Parasitol. 2020 Jun;36(6):560-570
pubmed: 32407682
J Clin Invest. 2020 Jun 1;130(6):2920-2927
pubmed: 32045385
Stem Cell Reports. 2017 Oct 10;9(4):1034-1042
pubmed: 28966117
Am J Trop Med Hyg. 2011 Feb;84(2 Suppl):4-11
pubmed: 21292872
mBio. 2018 May 8;9(3):
pubmed: 29739900
Cell Stem Cell. 2014 Aug 7;15(2):227-38
pubmed: 25017720
Antimicrob Agents Chemother. 2009 Oct;53(10):4533-6
pubmed: 19596869
Science. 1993 Aug 27;261(5125):1182-4
pubmed: 7689250