Optimization and use of near infrared imaging to guide lymph node collection in rhesus macaques (Macaca mulatta).
ICG
MLN
draining
fluorescence
imaging
indocyanine green
lymph node
macaque
mesenteric
targeted sampling
Journal
Journal of medical primatology
ISSN: 1600-0684
Titre abrégé: J Med Primatol
Pays: Denmark
ID NLM: 0320626
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
received:
24
05
2022
accepted:
28
06
2022
pubmed:
17
7
2022
medline:
14
9
2022
entrez:
16
7
2022
Statut:
ppublish
Résumé
Identification of lymph nodes (LNs) draining a specific site or in obese macaques can be challenging. Indocyanine Green (ICG) was administered intradermal (ID), intramuscular, in the oral mucosa, or subserosal in the colon followed by Near Infrared (NIR) imaging. After optimization to maximize LN identification, intradermal ICG was successful in identifying 50-100% of the axillary/inguinal LN at a site. Using NIR, collection of peripheral and mesenteric LNs in obese macaques was 100% successful after traditional methods failed. Additionally, guided collection of LNs draining the site of intraepithelial or intramuscular immunization demonstrated significantly increased numbers of T follicular helper (Tfh) cells in germinal centers of draining compared to nondraining LNs. These imaging techniques optimize our ability to evaluate immune changes within LNs over time, even in obese macaques. This approach allows for targeted serial biopsies that permit confidence that draining LNs are being harvested throughout the study.
Sections du résumé
BACKGROUND
Identification of lymph nodes (LNs) draining a specific site or in obese macaques can be challenging.
METHODS
Indocyanine Green (ICG) was administered intradermal (ID), intramuscular, in the oral mucosa, or subserosal in the colon followed by Near Infrared (NIR) imaging.
RESULTS
After optimization to maximize LN identification, intradermal ICG was successful in identifying 50-100% of the axillary/inguinal LN at a site. Using NIR, collection of peripheral and mesenteric LNs in obese macaques was 100% successful after traditional methods failed. Additionally, guided collection of LNs draining the site of intraepithelial or intramuscular immunization demonstrated significantly increased numbers of T follicular helper (Tfh) cells in germinal centers of draining compared to nondraining LNs.
CONCLUSION
These imaging techniques optimize our ability to evaluate immune changes within LNs over time, even in obese macaques. This approach allows for targeted serial biopsies that permit confidence that draining LNs are being harvested throughout the study.
Identifiants
pubmed: 35841132
doi: 10.1111/jmp.12605
pmc: PMC9474636
mid: NIHMS1821624
doi:
Substances chimiques
Indocyanine Green
IX6J1063HV
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
270-277Subventions
Organisme : NIH HHS
ID : P51 OD011092
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE026336
Pays : United States
Organisme : NIH HHS
ID : U42 OD010426
Pays : United States
Organisme : NIH HHS
ID : U42 OD023038
Pays : United States
Informations de copyright
© 2022 The Authors. Journal of Medical Primatology published by John Wiley & Sons Ltd.
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