Performance of TB-LAMP in the Diagnosis of Tuberculous Empyema Using Samples Obtained From Pleural Decortication.
Mycobacterium tuberculosis
TB empyema
diagnostic study
loop-mediated isothermal amplification assay (LAMP)
pleural tissue specimens
Journal
Frontiers in medicine
ISSN: 2296-858X
Titre abrégé: Front Med (Lausanne)
Pays: Switzerland
ID NLM: 101648047
Informations de publication
Date de publication:
2022
2022
Historique:
received:
20
02
2022
accepted:
30
05
2022
entrez:
18
7
2022
pubmed:
19
7
2022
medline:
19
7
2022
Statut:
epublish
Résumé
To evaluate the performance of TB-LAMP in the diagnosis of TB empyema using pleural tissue specimens obtained during pleural decortication. Using the clinical records and the different diagnostic test results of patients who underwent pleural decortication in a TB-designated hospital over 3.5 years, we calculated the sensitivity, specificity positive predictive, and negative predictive values of the pathology, MGIT 960 culture, and TB-LAMP obtained by using pleural tissue specimens against the etiologic diagnosis and composite clinical reference standard (CCRS) as the reference standards. A total of 304 patients' records were extracted. All these patients had gone through pleural decortication. When the etiologic diagnosis was used as the reference, the sensitivity of TB-LAMP in identifying TB empyema was 77.8% (compared to 10.6% of MGIT 960 TB-LAMP has great potential in faster and more accurate diagnosis of TB empyema. Our findings provide insights for optimizing diagnostic algorithms for TB empyema.
Identifiants
pubmed: 35847811
doi: 10.3389/fmed.2022.879772
pmc: PMC9278273
doi:
Types de publication
Journal Article
Langues
eng
Pagination
879772Informations de copyright
Copyright © 2022 Liu, Fan, Zhang, Hong, Ren, Tian and Chen.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Int J Infect Dis. 2021 Jul;108:89-95
pubmed: 33992762
J Infect Chemother. 2020 Aug;26(8):823-830
pubmed: 32409020
Respirology. 2018 Jul;23(7):714-717
pubmed: 29486527
Future Microbiol. 2017 Oct;12:1201-1218
pubmed: 28972418
Thorac Cardiovasc Surg. 2008 Mar;56(2):99-102
pubmed: 18278685
ANZ J Surg. 2021 Oct;91(10):2182-2187
pubmed: 34405522
Lancet Infect Dis. 2018 Jan;18(1):76-84
pubmed: 29198911
Sci Rep. 2019 Dec 20;9(1):19569
pubmed: 31863026
BMC Infect Dis. 2020 Nov 12;20(1):840
pubmed: 33183269
Respir Med. 2015 Sep;109(9):1188-92
pubmed: 26166016
Eur Respir J. 2013 Nov;42(5):1427-9
pubmed: 23900990
BMC Infect Dis. 2019 Mar 19;19(1):268
pubmed: 30890135
Semin Respir Crit Care Med. 2001 Dec;22(6):637-46
pubmed: 16088708
BMC Infect Dis. 2020 Jan 6;20(1):14
pubmed: 31906996
Thorax. 2006 Sep;61(9):783-90
pubmed: 16738037
Ann Thorac Cardiovasc Surg. 2015;21(3):223-8
pubmed: 25818121
Thorax. 2015 Oct;70(10):995-7
pubmed: 25997433
Ann Thorac Surg. 2017 Nov;104(5):1688-1694
pubmed: 28964422