Laser-enabled delivery of antiretroviral drugs into HIV-1 infected TZM-bl cells.
Chinese Hamster ovary cells
Gaussian beam
TZM-bl cells
antiretroviral drugs
femtosecond laser
human immunodeficiency virus
Journal
Journal of biophotonics
ISSN: 1864-0648
Titre abrégé: J Biophotonics
Pays: Germany
ID NLM: 101318567
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
revised:
15
07
2022
received:
17
02
2022
accepted:
18
07
2022
pubmed:
20
7
2022
medline:
6
10
2022
entrez:
19
7
2022
Statut:
ppublish
Résumé
The use of femtosecond laser to create sub-microscopic transient pores on the cell membrane allowing exogenous material into mammalian cells has become a very efficient optical delivery method over the past decade. This study focuses on laser-enabled delivery of antiretroviral (ARV) drugs into HIV-1 infected TZM-bl cells in vitro. A 1 kHz femtosecond laser emitting at a wavelength of 800 nm was used to photoporate cells at 6.5 μW. Trypan blue was used for characterisation and its uptake was quantified using Matlab software. Cell membrane damage was assessed using the lactate dehydrogenase (LDH) assay while HIV-1 infection was assessed using luciferase assay. Our results showed successful delivery of ARVs into HIV-1 infected cells without compromising their cell membranes, subsequently reducing the level of infection. The LDH assay showed no significant cell membrane damage of laser-treated cells, and the luciferase assay demonstrated significant reduction in the level of HIV-1 infection.
Identifiants
pubmed: 35852044
doi: 10.1002/jbio.202200043
doi:
Substances chimiques
Pharmaceutical Preparations
0
Lactate Dehydrogenases
EC 1.1.-
Luciferases
EC 1.13.12.-
Trypan Blue
I2ZWO3LS3M
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202200043Subventions
Organisme : National Research Foundation (NRF)
Organisme : Department of Science and Innovation (DSI)
Organisme : University of South Africa (UNISA)
Organisme : Council for Scientific and Industrial Research (CSIR)
Informations de copyright
© 2022 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.
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