Covalent and Oriented Immobilization of Antibodies through Systematic Modification of Photoactivatable RNA Hybrid Aptamers.
Journal
Bioconjugate chemistry
ISSN: 1520-4812
Titre abrégé: Bioconjug Chem
Pays: United States
ID NLM: 9010319
Informations de publication
Date de publication:
17 08 2022
17 08 2022
Historique:
pubmed:
20
7
2022
medline:
19
8
2022
entrez:
19
7
2022
Statut:
ppublish
Résumé
We report a covalent and well-oriented strategy of immobilization of antibodies using photoactivatable Fc-binding RNA aptamers (FcBAs). We prepared several types of FcBAs that were further modified with photoaffinity probes (i.e., benzophenone or diazirine), and evaluated the binding capabilities and the photo-crosslinking efficiency of them via pull-down assays and fluorescence analyses, respectively. Initial moderate photo-crosslinking efficiency (
Identifiants
pubmed: 35853199
doi: 10.1021/acs.bioconjchem.2c00274
doi:
Substances chimiques
Aptamers, Nucleotide
0
Immunoglobulin G
0
Diazomethane
60A625P70P
RNA
63231-63-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM