Changes of physico-chemical properties of nano-biomaterials by digestion fluids affect the physiological properties of epithelial intestinal cells and barrier models.
Biotransformation
Caco-2
Gastro-intestinal barrier
HCT116
HCoEpiC
In vitro simulated digestion
Inflammation
Nano-biomaterials
Permeability
Toxicity
Journal
Particle and fibre toxicology
ISSN: 1743-8977
Titre abrégé: Part Fibre Toxicol
Pays: England
ID NLM: 101236354
Informations de publication
Date de publication:
19 07 2022
19 07 2022
Historique:
received:
09
02
2022
accepted:
29
06
2022
entrez:
19
7
2022
pubmed:
20
7
2022
medline:
22
7
2022
Statut:
epublish
Résumé
The widespread use of nano-biomaterials (NBMs) has increased the chance of human exposure. Although ingestion is one of the major routes of exposure to NBMs, it is not thoroughly studied to date. NBMs are expected to be dramatically modified following the transit into the oral-gastric-intestinal (OGI) tract. How these transformations affect their interaction with intestinal cells is still poorly understood. NBMs of different chemical nature-lipid-surfactant nanoparticles (LSNPs), carbon nanoparticles (CNPs), surface modified Fe The application of the in vitro SDHS modified the biocompatibility of NBMs on gastrointestinal cells. The differences between SHDS-treated and untreated NBMs could be attributed to the irreversible modification of the NBMs in the SHDS. Aggregation was detected for all NBMs regardless of their chemical nature, while pH- or enzyme-mediated partial degradation was detected for hydroxyapatite or polymer-coated iron oxide nanoparticles and lipid nanoparticles, respectively. The formation of a bio-corona, which contains proteases, was also demonstrated on all the analysed NBMs. In viability assays, undifferentiated primary cells were more sensitive than immortalised cells to digested NBMs, but neither pristine nor treated NBMs affected the intestinal barrier viability and permeability. SHDS-treated NBMs up-regulated the tight junction genes (claudin 3 and 5, occludin, zonula occludens 1) in intestinal barrier, with different patterns between each NBM, and increase the expression of both pro- and anti-inflammatory cytokines (IL-1β, TNF-α, IL-22, IL-10). Notably, none of these NBMs showed any significant genotoxic effect. Overall, the results add a piece of evidence on the importance of applying validated in vitro SHDS models for the assessment of NBM intestinal toxicity/biocompatibility. We propose the association of chemical and microscopic characterization, SHDS and in vitro tests on both immortalised and primary cells as a robust screening pipeline useful to monitor the changes in the physico-chemical properties of ingested NBMs and their effects on intestinal cells.
Sections du résumé
BACKGROUND
The widespread use of nano-biomaterials (NBMs) has increased the chance of human exposure. Although ingestion is one of the major routes of exposure to NBMs, it is not thoroughly studied to date. NBMs are expected to be dramatically modified following the transit into the oral-gastric-intestinal (OGI) tract. How these transformations affect their interaction with intestinal cells is still poorly understood. NBMs of different chemical nature-lipid-surfactant nanoparticles (LSNPs), carbon nanoparticles (CNPs), surface modified Fe
RESULTS
The application of the in vitro SDHS modified the biocompatibility of NBMs on gastrointestinal cells. The differences between SHDS-treated and untreated NBMs could be attributed to the irreversible modification of the NBMs in the SHDS. Aggregation was detected for all NBMs regardless of their chemical nature, while pH- or enzyme-mediated partial degradation was detected for hydroxyapatite or polymer-coated iron oxide nanoparticles and lipid nanoparticles, respectively. The formation of a bio-corona, which contains proteases, was also demonstrated on all the analysed NBMs. In viability assays, undifferentiated primary cells were more sensitive than immortalised cells to digested NBMs, but neither pristine nor treated NBMs affected the intestinal barrier viability and permeability. SHDS-treated NBMs up-regulated the tight junction genes (claudin 3 and 5, occludin, zonula occludens 1) in intestinal barrier, with different patterns between each NBM, and increase the expression of both pro- and anti-inflammatory cytokines (IL-1β, TNF-α, IL-22, IL-10). Notably, none of these NBMs showed any significant genotoxic effect.
CONCLUSIONS
Overall, the results add a piece of evidence on the importance of applying validated in vitro SHDS models for the assessment of NBM intestinal toxicity/biocompatibility. We propose the association of chemical and microscopic characterization, SHDS and in vitro tests on both immortalised and primary cells as a robust screening pipeline useful to monitor the changes in the physico-chemical properties of ingested NBMs and their effects on intestinal cells.
Identifiants
pubmed: 35854319
doi: 10.1186/s12989-022-00491-w
pii: 10.1186/s12989-022-00491-w
pmc: PMC9297619
doi:
Substances chimiques
Biocompatible Materials
0
Hydroxyapatites
0
Lipid Nanoparticles
0
Liposomes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
49Informations de copyright
© 2022. The Author(s).
Références
Faraday Discuss. 2020 Jun 19;222(0):332-349
pubmed: 32101206
Mol Pharm. 2017 Nov 6;14(11):3750-3761
pubmed: 28945434
Part Fibre Toxicol. 2017 Oct 13;14(1):40
pubmed: 29029643
ACS Nano. 2018 Aug 28;12(8):8115-8128
pubmed: 30021067
NanoImpact. 2021 Apr;22:100306
pubmed: 35559963
Colloids Surf B Biointerfaces. 2013 Feb 1;102:257-64
pubmed: 23010116
Talanta. 2021 Oct 1;233:122494
pubmed: 34215112
PLoS One. 2021 Jul 23;16(7):e0255120
pubmed: 34297768
J Gastroenterol. 1999 Feb;34(1):66-74
pubmed: 10204613
Mutat Res Genet Toxicol Environ Mutagen. 2019 Sep;845:402980
pubmed: 31561898
Proc Natl Acad Sci U S A. 2019 Mar 19;116(12):5362-5369
pubmed: 30837316
Food Funct. 2021 Jul 5;12(13):5975-5988
pubmed: 34032251
Part Fibre Toxicol. 2017 Nov 25;14(1):47
pubmed: 29178961
ACS Biomater Sci Eng. 2016 Sep 12;2(9):1608-1618
pubmed: 33440594
Langmuir. 2018 May 22;34(20):5679-5695
pubmed: 29672062
Nature. 2015 Dec 24;528(7583):560-564
pubmed: 26649819
Bull Exp Biol Med. 2020 Apr;168(6):785-788
pubmed: 32328944
Arch Toxicol. 2020 Apr;94(4):1191-1202
pubmed: 32162006
Food Funct. 2014 Jun;5(6):1278-85
pubmed: 24741679
J Colloid Interface Sci. 2019 Jun 15;546:130-138
pubmed: 30913487
Environ Sci Nano. 2016 Dec 1;3(6):1510-1520
pubmed: 28357114
Sci Rep. 2019 Dec 27;9(1):20180
pubmed: 31882911
Nanotoxicology. 2010 Dec;4:347-63
pubmed: 20858045
Langmuir. 2014 Sep 30;30(38):11442-52
pubmed: 25137213
ACS Nano. 2010 Dec 28;4(12):7481-91
pubmed: 21082814
Int J Mol Sci. 2020 Feb 27;21(5):
pubmed: 32120830
Food Sci Technol Int. 2020 Jun;26(4):353-366
pubmed: 31870192
J Cell Sci. 1992 Jul;102 ( Pt 3):581-600
pubmed: 1506435
Small. 2020 May;16(21):e1907640
pubmed: 32196921
Artif Cells Nanomed Biotechnol. 2018;46(sup2):993-1002
pubmed: 29842790
Nanotoxicology. 2013 Nov;7(7):1198-210
pubmed: 22931191
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2018 Jan;10(1):
pubmed: 28548289
ALTEX. 2012;29(3):275-85
pubmed: 22847255
Chemosphere. 2021 Apr;268:128843
pubmed: 33172667
Int J Pharm. 2017 Aug 30;529(1-2):474-485
pubmed: 28684364
Science. 2006 Feb 3;311(5761):622-7
pubmed: 16456071
Gastroenterology. 2019 Jun;156(8):2281-2296.e6
pubmed: 30779922
Materials (Basel). 2019 Nov 21;12(23):
pubmed: 31766412
Small. 2021 Apr;17(15):e2004630
pubmed: 33475244
Nanomaterials (Basel). 2020 Oct 27;10(11):
pubmed: 33120920
Nanomaterials (Basel). 2020 Jan 22;10(2):
pubmed: 31978987
Curr Med Chem. 2017;24(22):2423-2438
pubmed: 27804879
Nanotoxicology. 2013 Jun;7(4):353-66
pubmed: 22394261
Biochim Biophys Acta. 2014 Mar;1840(3):1171-80
pubmed: 24361607
Front Immunol. 2021 Sep 14;12:684605
pubmed: 34594323
Sci Rep. 2019 Dec 18;9(1):19358
pubmed: 31852946
Front Toxicol. 2021 Mar 22;3:636976
pubmed: 35295141
Chem Res Toxicol. 2020 May 18;33(5):1226-1236
pubmed: 32319286
Curr Comput Aided Drug Des. 2016;12(4):259-264
pubmed: 27559000
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2013 Mar-Apr;5(2):111-29
pubmed: 23335558
Eur J Pharm Sci. 2019 Jan 15;127:115-120
pubmed: 30393196
Adv Drug Deliv Rev. 2021 Dec;179:114021
pubmed: 34710529
J Drug Target. 2008 Jan;16(1):36-42
pubmed: 18172818
Drug Dev Ind Pharm. 2015;41(10):1582-8
pubmed: 25342478
ACS Nano. 2017 May 23;11(5):4542-4552
pubmed: 28443337
Int J Occup Environ Health. 2014 Jul-Sep;20(3):220-34
pubmed: 25000110
Environ Res. 2021 Feb;193:110536
pubmed: 33253701
Part Fibre Toxicol. 2014 Mar 25;11:13
pubmed: 24666995
Front Immunol. 2019 Aug 28;10:2057
pubmed: 31555282
Anal Chem. 2020 Sep 15;92(18):12257-12264
pubmed: 32786449
J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2019;37(2):116-131
pubmed: 31230526
Nanotoxicology. 2016;10(1):53-62
pubmed: 25738417
Methods Mol Biol. 2013;1025:137-55
pubmed: 23918335
BMC Cancer. 2010 Mar 30;10:119
pubmed: 20350328
Nat Mater. 2016 Nov;15(11):1212-1221
pubmed: 27525571
Cancer Lett. 2017 Mar 1;388:262-268
pubmed: 27998763
Pharm Res. 2006 Mar;23(3):557-64
pubmed: 16388405
Toxicol In Vitro. 2020 Sep;67:104903
pubmed: 32473318
World J Gastroenterol. 2017 Mar 28;23(12):2106-2123
pubmed: 28405139
Biosci Biotechnol Biochem. 2002 Nov;66(11):2449-57
pubmed: 12506986
Nanotoxicology. 2012 Feb;6(1):36-46
pubmed: 21309618
Toxicol In Vitro. 2012 Dec;26(8):1247-51
pubmed: 22123491
Nat Biomed Eng. 2020 Jan;4(1):84-96
pubmed: 31686002
Cell Biol Toxicol. 2005 Jan;21(1):1-26
pubmed: 15868485
Food Funct. 2021 Mar 21;12(6):2760-2771
pubmed: 33683238
Biotechnol Prog. 2006 Sep-Oct;22(5):1294-300
pubmed: 17022666
Annu Rev Pathol. 2010;5:119-44
pubmed: 20078218
Drug Deliv. 2017;24(sup1):94-107
pubmed: 29124979
Food Res Int. 2021 Jul;145:110425
pubmed: 34112427
Mutagenesis. 2017 Jan;32(1):139-149
pubmed: 27789654
Cell. 1993 Oct 22;75(2):263-74
pubmed: 8402911
Small. 2020 Sep;16(36):e2001246
pubmed: 32495486
Nat Protoc. 2016 Dec;11(12):2301-2319
pubmed: 27809316
Carbohydr Polym. 2021 Jan 1;251:117111
pubmed: 33142648
Nanoscale. 2015 Oct 28;7(40):16647-57
pubmed: 26154822
Nanotoxicology. 2020 Feb;14(1):111-126
pubmed: 31648587
ACS Nano. 2011 Sep 27;5(9):7503-9
pubmed: 21861491
J Appl Toxicol. 2013 Nov;33(11):1316-29
pubmed: 23606564
Circ Res. 2018 Jan 19;122(2):296-309
pubmed: 29118058
J Biomed Mater Res A. 2010 Apr;93(1):247-57
pubmed: 19557787
Intensive Care Med Exp. 2018 Sep 26;6(1):37
pubmed: 30259344
Crit Rev Food Sci Nutr. 2014;54(11):1427-57
pubmed: 24580539
Anal Biochem. 2014 Jun 1;454:36-7
pubmed: 24632099
Int J Nanomedicine. 2019 Nov 21;14:9077-9088
pubmed: 31819420
Part Fibre Toxicol. 2018 Jul 3;15(1):29
pubmed: 29970114
J Ginseng Res. 2018 Jul;42(3):327-333
pubmed: 29983614
J Gastroenterol Hepatol. 2008 Dec;23 Suppl 2:S146-50
pubmed: 19120888
Part Fibre Toxicol. 2021 Feb 17;18(1):8
pubmed: 33596948
Colloids Surf B Biointerfaces. 2018 Dec 1;172:395-399
pubmed: 30195156
J Immunol. 2019 Feb 1;202(3):956-965
pubmed: 30617224
Appl Biochem Biotechnol. 2012 Aug;167(7):2076-87
pubmed: 22669687
Sci Rep. 2019 May 9;9(1):7135
pubmed: 31073210
J Sci Food Agric. 2020 Oct;100(13):4950-4958
pubmed: 32484244
Gut. 2007 Jan;56(1):61-72
pubmed: 16822808
Adv Colloid Interface Sci. 2014 Nov;213:36-47
pubmed: 25307126
J Control Release. 2014 Oct 28;192:219-27
pubmed: 25058571
Nat Methods. 2016 Sep;13(9):731-40
pubmed: 27348712
Sci Rep. 2020 Nov 20;10(1):20290
pubmed: 33219331
Nanomedicine. 2018 Feb;14(2):353-364
pubmed: 29157980
NanoImpact. 2020 Oct;20:
pubmed: 33344797
Eur J Pharm Sci. 2019 Apr 30;132:72-85
pubmed: 30797937
Nanotoxicology. 2017 Aug;11(6):751-761
pubmed: 28671030
Gut. 2017 Jul;66(7):1216-1224
pubmed: 26848183
PLoS One. 2015 May 21;10(5):e0127174
pubmed: 25996496
J Appl Toxicol. 2019 Aug;39(8):1155-1163
pubmed: 31017309
Front Biosci (Landmark Ed). 2009 Jan 01;14(7):2765-78
pubmed: 19273235
J Nat Med. 2018 Sep;72(4):897-904
pubmed: 29797179
Front Immunol. 2018 Dec 06;9:2883
pubmed: 30574151
Part Fibre Toxicol. 2010 Nov 30;7(1):36
pubmed: 21118529
J Am Chem Soc. 2010 Apr 28;132(16):5761-8
pubmed: 20356039
J Appl Toxicol. 2021 Feb;41(2):291-302
pubmed: 33107989
Front Immunol. 2019 Oct 16;10:2346
pubmed: 31749791
Free Radic Biol Med. 2019 Apr;134:165-176
pubmed: 30639569
Environ Sci Technol. 2017 Feb 7;51(3):1259-1266
pubmed: 28075572
Int J Nanomedicine. 2018 Mar 15;13(T-NANO 2014 Abstracts):47-50
pubmed: 29593394