Myristic acid selectively augments β-tubulin levels in C2C12 myotubes via diacylglycerol kinase δ.
diacylglycerol kinase
myotube
myristic acid
type II diabetes
β-tubulin
Journal
FEBS open bio
ISSN: 2211-5463
Titre abrégé: FEBS Open Bio
Pays: England
ID NLM: 101580716
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
revised:
29
06
2022
received:
17
04
2022
accepted:
19
07
2022
pubmed:
21
7
2022
medline:
5
10
2022
entrez:
20
7
2022
Statut:
ppublish
Résumé
Effective amelioration of type II diabetes requires therapies that increase both glucose uptake activity per cell and skeletal muscle mass. Myristic acid (14:0) increases diacylglycerol kinase (DGK) δ protein levels and enhances glucose uptake in myotubes in a DGKδ-dependent manner. However, it is still unclear whether myristic acid treatment affects skeletal muscle mass. In this study, we found that myristic acid treatment increased the protein level of β-tubulin, which constitutes microtubules and is closely related to muscle mass, in C2C12 myotubes but not in the proliferation stage in C2C12 myoblasts. However, lauric (12:0), palmitic (16:0) and oleic (18:1) acids failed to affect DGKδ and β-tubulin protein levels in C2C12 myotubes. Moreover, knockdown of DGKδ by siRNA significantly inhibited the increased protein level of β-tubulin in the presence of myristic acid, suggesting that the increase in β-tubulin protein by myristic acid depends on DGKδ. These results indicate that myristic acid selectively affects β-tubulin protein levels in C2C12 myotubes via DGKδ, suggesting that this fatty acid improves skeletal muscle mass in addition to increasing glucose uptake activity per cell.
Identifiants
pubmed: 35856166
doi: 10.1002/2211-5463.13466
pmc: PMC9527581
doi:
Substances chimiques
RNA, Small Interfering
0
Tubulin
0
Myristic Acid
0I3V7S25AW
Diacylglycerol Kinase
EC 2.7.1.107
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1788-1796Informations de copyright
© 2022 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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