Bone morphogenetic protein-4 system expression in human coronary artery endothelial and smooth muscle cells under dynamic flow: effect of medicated bioresorbable vascular scaffolds at low and normal shear stress.


Journal

Heart and vessels
ISSN: 1615-2573
Titre abrégé: Heart Vessels
Pays: Japan
ID NLM: 8511258

Informations de publication

Date de publication:
Dec 2022
Historique:
received: 01 02 2022
accepted: 06 07 2022
pubmed: 21 7 2022
medline: 21 10 2022
entrez: 20 7 2022
Statut: ppublish

Résumé

Endothelial and smooth muscle cell dysfunction is an early event at the onset of atherosclerosis, a heterogeneous and multifactorial pathology of the vascular wall. Bone morphogenetic protein (BMP)-4, a mechanosensitive autocrine cytokine, and BMPR-1a, BMPR-1b, BMPR-2 specific receptors play a key role in atherosclerotic plaque formation and vascular calcification and BMP4 is regarded as a biomarker of endothelial cell activation. The study aimed to examine the BMP4 system expression by Real-Time PCR in Human Coronary Artery Endothelial (HCAECs) and Smooth Muscle Cells (HCASMCs) under different flow rates determining low or physiological shear stress in the presence/absence of medicated Bioresorbable Vascular Scaffold (BVS). The HCAEC and HCASMC were subjected to 1-10-20 dyne/cm

Identifiants

pubmed: 35857064
doi: 10.1007/s00380-022-02140-w
pii: 10.1007/s00380-022-02140-w
doi:

Substances chimiques

Everolimus 9HW64Q8G6G
RNA, Messenger 0
Biomarkers 0
Cytokines 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2137-2149

Subventions

Organisme : Horizon 2020 Framework Programme
ID : 777119

Informations de copyright

© 2022. Springer Japan KK, part of Springer Nature.

Références

Glass CK, Witztum JL (2001) Atherosclerosis: the road ahead. Cell 104:503–516
pubmed: 11239408 doi: 10.1016/S0092-8674(01)00238-0
Stary HC, Chandler AB, Glagov S, Guyton JR, Insull W, Rosenfeld ME, Schaffer A, Schwartz CJ, Wagner WD, Wissler RW (1994) A definition of initial, fatty streak, and intermediate lesions of atherosclerosis: a report from the committee on vascular lesions of the council on arteriosclerosis. Circulation 89:2462–2478
pubmed: 8181179 doi: 10.1161/01.CIR.89.5.2462
Hansson GK (2005) Inflammation, atherosclerosis and coronary artery disease. N Engl J Med 352:1685–1695
pubmed: 15843671 doi: 10.1056/NEJMra043430
Ketelhuth DFJ, Hansson GK (2011) Cellular immunity, low-density lipoprotein and atherosclerosis: break of tolerance in the artery wall. Thromb Haemos 106:779–786
doi: 10.1160/TH11-05-0321
Frangos SG, Gahtan V, Sumpio B (1999) Localization of atherosclerosis: role of hemodynamics. Arch Surg 134:1142–1149
pubmed: 10522862 doi: 10.1001/archsurg.134.10.1142
Ross R, Glomset JA (1976) The pathogenesis of atherosclerosis (first of two parts). N Engl J Med 295:369–377
pubmed: 819830 doi: 10.1056/NEJM197608122950707
Ross R (1999) Atherosclerosis—an inflammatory disease. N Engl J Med 340:115–126
pubmed: 9887164 doi: 10.1056/NEJM199901143400207
Malek AM, Alper SL, Izumo S (1999) Hemodynamic shear stress and its role in atherosclerosis. JAMA 282:2035–2042
pubmed: 10591386 doi: 10.1001/jama.282.21.2035
Gimbrone MA, Guillermo GC (2016) Endothelial cell dysfunction and pathobiology of atherosclerosis. Circ Res 118:620–636
pubmed: 26892962 pmcid: 4762052 doi: 10.1161/CIRCRESAHA.115.306301
Miyauchi T, Masaki T (1999) Pathophysiology of endothelin in the cardiovascular system. Ann Rev Physiol 61:391–415
doi: 10.1146/annurev.physiol.61.1.391
Topper JN, Cai J, Falb D, Gimbrone MA (1996) Identification of vascular endothelial genes differentially responsive to fluid mechanical stimuli: cyclooxygenase-2, manganese superoxide dismutase, and endothelial cell nitric oxide synthase are selectively up-regulated by steady laminar shear stress. Proc Natl Acad Sci 93:10417–10422
pubmed: 8816815 pmcid: 38399 doi: 10.1073/pnas.93.19.10417
Csiszar A, Lehoux S, Ungvari Z (2009) Hemodynamic forces, vascular oxidative stress, and regulation of BMP-2/4 expression. Antioxid Redox Signal 11:1683–1697
pubmed: 19320562 pmcid: 2842584 doi: 10.1089/ars.2008.2401
Miyazono K, Kamiya Y, Morikawa M (2010) Bone morphogenetic protein receptors and signal transduction. J Biochem 147:35–51
pubmed: 19762341 doi: 10.1093/jb/mvp148
Sorescu GP, Sykes M, Weiss D, Platt MO, Saha A, Hwang J, Boyd N, Boo YC, Vega JD, Taylor WR, Jo H (2003) Bone morphogenic protein 4 produced in endothelial cells by oscillatory shear stress stimulates an inflammatory response. J Biol Chem 278:31128–31135
pubmed: 12766166 doi: 10.1074/jbc.M300703200
Sorescu GP, Song H, Tressel SL, Hwang J, Dikalov S, Smith DA, Boyd NL, Platt MO, Lassègue B, Griendling KK, Jo H (2004) Bone morphogenic protein 4 produced in endothelial cells by oscillatory shear stress induces monocyte adhesion by stimulating reactive oxygen species production from a Nox1-based NADPH oxidase. Circ Res 95:773–779
pubmed: 15388638 doi: 10.1161/01.RES.0000145728.22878.45
Jo H, Song H, Mowbray A (2006) Role of NADPH oxidases in disturbed flow- and BMP4-induced inflammation and atherosclerosis. Antioxid Redox Sign 8:1609–1619
doi: 10.1089/ars.2006.8.1609
Lüscher TF, Steffel J, Eberli FR, Joner M, Nakazawa G, Tanner FC, Virmani R (2007) Drug-eluting stent and coronary thrombosis: biological mechanisms and clinical implications. Circulation 115:1051–1058
pubmed: 17325255 doi: 10.1161/CIRCULATIONAHA.106.675934
Buccheri D, Piraino D, Andolina G, Cortese B (2016) Understanding and managing in-stent restenosis: a review of clinical data, from pathogenesis to treatment. J Thorac Dis 8:E1150–E1162
pubmed: 27867580 pmcid: 5107494 doi: 10.21037/jtd.2016.10.93
Gogas BD (2014) Bioresorbable scaffolds for percutaneous coronary interventions. Glob Cardiol Sci Pract 2014:409–427
pubmed: 25780795 pmcid: 4355515
Brugaletta S, Heo JH, Garcia-Garcia HM, Farooq V, van Geuns RJ, de Bruyne B, Dudek D, Smits PC, Koolen J, McClean D, Dorange C, Veldhof S, Rapoza R, Onuma Y, Bruining N, Ormiston JA, Serruys PW (2012) Endothelial-dependent vasomotion in a coronary segment treated by ABSORB everolimus-eluting bioresorbable vascular scaffold system is related to plaque composition at the time of bioresorption of the polymer: indirect finding of vascular reparative therapy? Eur Heart J 33:1325–1333
pubmed: 22507972 doi: 10.1093/eurheartj/ehr466
Vozzi F, Cecchettini A, Cabiati M, Mg F, Aretini P, Del Ry S, Rocchiccioli S, Pelosi G (2021) Modulated molecular markers of restenosis and thrombosis byin-vitrovascular cells exposed to bioresorbable scaffolds. Biomed Mater 16(4):045039
doi: 10.1088/1748-605X/ac0401
Fejes Z, Czimmerer Z, Szük T, Póliska S, Horváth A, Balogh E, Jeney V, Váradi J, Fenyvesi F, Balla G, Édes I, Balla J, Kappelmayer J, Nagy B Jr (2018) Endothelial cell activation is attenuated by everolimus via transcriptional and post-transcriptional regulatory mechanisms after drug-eluting coronary stenting. PLoSOne 13:e0197890
doi: 10.1371/journal.pone.0197890
Tarbell JM, Shi ZD (2013) Effect of the glycocalyx layer on transmission of interstitial flow shear stress to embedded cells. Biomech Model Mechanobiol 12:111–121
pubmed: 22411016 doi: 10.1007/s10237-012-0385-8
Shi ZD, Tarbell JM (2011) Fluid flow mechano-transduction in vascular smooth muscle cells and fibroblasts. Ann Biomed Engin 39:1608–1619
doi: 10.1007/s10439-011-0309-2
Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55:611–622
pubmed: 19246619 doi: 10.1373/clinchem.2008.112797
Anders S, Huber W (2010) Differential expression analysis for sequence count data. Genome Biol 11:R106-112
pubmed: 20979621 pmcid: 3218662 doi: 10.1186/gb-2010-11-10-r106
Love MI, Huber W, Anders S (2014) Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol 15:550
pubmed: 25516281 pmcid: 4302049 doi: 10.1186/s13059-014-0550-8
Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3:research0034
pubmed: 12184808 pmcid: 126239 doi: 10.1186/gb-2002-3-7-research0034
Hansson GK, Hermansson A (2011) The immune system in atherosclerosis. Nature Immunol 12:204–212
doi: 10.1038/ni.2001
Skålén K, Gustafsson M, Rydberg EK, Hultén LM, Wiklund O, Innerarity TL, Borén J (2002) Subendothelial retention of atherogenic lipoproteins in early atherosclerosis. Nature 417:750–754
pubmed: 12066187 doi: 10.1038/nature00804
Ross R (1993) The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature 362:801–809
pubmed: 8479518 doi: 10.1038/362801a0
Epstein SE, Zhou YF, Zhu J (1999) Infection and atherosclerosis: emerging mechanistic paradigms. Circulation 100(4):e20–e28
pubmed: 10421626 doi: 10.1161/01.CIR.100.4.e20
Lamon BD, Hajjar DP (2008) Inflammation at the molecular interface of atherogenesis. Am J Pathol 173:1253–1264
pubmed: 18948435 pmcid: 2570117 doi: 10.2353/ajpath.2008.080442
Marzilli M, Merz CN, Boden WE, Bonow RO, Capozza PG, Chilian WM, DeMaria AN, Guarini G, Huqi A, Morrone D, Patel MR, Weintraub WS (2012) Obstructive coronary atherosclerosis and ischemic heart disease: an elusive link! J Am Coll Cardiol 60:951–956
pubmed: 22954239 doi: 10.1016/j.jacc.2012.02.082
Wong WT, Tian XY, Chen Y, Leung FP, Liu L, Lee HK, Ng CF, Xu A, Yao X, Vanhoutte PM, Tipoe GL, Huang Y (2010) Bone morphogenic protein-4 impairs endothelial function through oxidative stress-dependent cyclooxygenase-2 upregulation: implications on hypertension. Circ Res 107:984–991
pubmed: 20724703 doi: 10.1161/CIRCRESAHA.110.222794
Tian XY, Yung LH, Wong WT, Liu J, Leung FP, Liu L, Chen Y, Kong SK, Kwan KM, Ng SM, Lai PB, Yung LM, Yao X, Huang Y (2012) Bone morphogenic protein-4 induces endothelial cell apoptosis through oxidative stress-dependent p38MAPK and JNK pathway. J Mol Cell Cardiol 52:237–244
pubmed: 22064324 doi: 10.1016/j.yjmcc.2011.10.013
Jo H, Song H (2005) Disturbed shear stress induces inflammation and atherosclerosis-role of BMP4 as a mechanosensitive and inflammatory cytokine. J Korean Diabetes 29:271–281
Yin W, Jo H, Voit EO (2010) Systems analysis of the role of bone morphogenic protein 4 in endothelial inflammation. Ann Biomed Eng 38:291–307
pubmed: 19851868 doi: 10.1007/s10439-009-9822-y
Csiszar A, Lehoux S, Ungvari Z (2009) Hemodynamic forces, vascular oxidative stress, and regulation of BMP-2/4 expression. Antioxid Redox Signal 11:1683–1697
pubmed: 19320562 pmcid: 2842584 doi: 10.1089/ars.2008.2401
Cho A, Mitchell L, Koopmans D, Langille BL (1997) Effects of changes in blood flow rate on cell death and cell proliferation in carotid arteries of immature rabbits. Circ Res 81:328–337
pubmed: 9285634 doi: 10.1161/01.RES.81.3.328
Falk E (2006) Pathogenesis of atherosclerosis. J Am Coll Cardiol 47:C7-12
pubmed: 16631513 doi: 10.1016/j.jacc.2005.09.068
Walshe TE, Ferguson G, Connell P, O’Brien C, Cahill PA (2005) Pulsatile flow increases the expression of eNOS, ET-1, and prostacyclin in a novel in vitro coculture model of the retinal vasculature. Invest Ophthalmol Vis Sci 46:375–382
pubmed: 15623798 doi: 10.1167/iovs.04-0806
Urschel K, Cicha I, Daniel WG, Garlichs CD (2012) Shear stress patterns affect the secreted chemokine profile in endothelial cells. Clin Hemorheol Microcirc 50:143–152
pubmed: 22538542 doi: 10.3233/CH-2011-1450
Wang DM, Tarbell JM (1995) Modeling interstitial flow in an artery wall allows estimation of wall shear stress on smooth muscle cells. J Biomech Eng 117:358–363
pubmed: 8618390 doi: 10.1115/1.2794192
Kurdi A, Roth L, Van der Veken B, Van Dam D, De Deyn PP, De Doncker M, Neels H, De Meyer GRY, Martinet W (2019) Everolimus depletes plaque macrophages, abolishes intraplaque neovascularization and improves survival in mice with advanced atherosclerosis. Vascul Pharmacol 113:70–76
pubmed: 30590134 doi: 10.1016/j.vph.2018.12.004
Moore JE Jr, Berry JL (2002) Fluid and solid mechanical implications of vascular stenting. Ann Biomed Eng 30:498–508
pubmed: 12086001 doi: 10.1114/1.1458594
He Y, Duraiswamy N, Frank AO, Moore JE Jr (2005) Blood flow in stented arteries: a parametric comparison of strut design patterns in three dimensions. J Biomech Eng 127:637–647
pubmed: 16121534 doi: 10.1115/1.1934122
Mohler ER 3rd, Gannon F, Reynolds C, Zimmerman R, Keane MG, Kaplan FS (2001) Bone formation and inflammation in cardiac valves. Circulation 103:1522–1528
pubmed: 11257079 doi: 10.1161/01.CIR.103.11.1522
Dhore CR, Cleutjens JP, Lutgens E, Cleutjens KB, Geusens PP, Kitslaar PJ, Tordoir JH, Spronk HM, Vermeer C, Daemen MJ (2001) Differential expression of bone matrix regulatory proteins in human atherosclerotic plaques. Arterioscler Thromb Vasc Biol 21:1998–2003
pubmed: 11742876 doi: 10.1161/hq1201.100229
Tarbell JM, Shi ZD, Dunn J, Jo H (2014) Fluid mechanics, arterial disease, and gene expression. Ann Rev Fluid Mech 46:591–614
doi: 10.1146/annurev-fluid-010313-141309
Cunningham KS, Gotlieb AI (2005) The role of shear stress in the pathogenesis of atherosclerosis. Lab Invest 85:9–23
pubmed: 15568038 doi: 10.1038/labinvest.3700215

Auteurs

Manuela Cabiati (M)

Laboratory of Biochemistry and Molecular Biology, Institute of Clinical Physiology CNR, Via Giuseppe Moruzzi 1, 56124, Pisa, Italy. manuela.cabiati@ifc.cnr.it.

Manuel Giacomarra (M)

Laboratory of Biochemistry and Molecular Biology, Institute of Clinical Physiology CNR, Via Giuseppe Moruzzi 1, 56124, Pisa, Italy.

Martina Fontanini (M)

Laboratory of Biochemistry and Molecular Biology, Institute of Clinical Physiology CNR, Via Giuseppe Moruzzi 1, 56124, Pisa, Italy.

Antonella Cecchettini (A)

Laboratory of Proteomics, Institute of Clinical Physiology, IFC-CNR, Pisa, Italy.
Department of Experimental and Clinical Medicine, University of Pisa, Pisa, Italy.

Gualtiero Pelosi (G)

Laboratory of Biomimetic Materials and Tissue Engineering, Institute of Clinical Physiology CNR, Pisa, Italy.

Federico Vozzi (F)

Laboratory of Biomimetic Materials and Tissue Engineering, Institute of Clinical Physiology CNR, Pisa, Italy.

Silvia Del Ry (S)

Laboratory of Biochemistry and Molecular Biology, Institute of Clinical Physiology CNR, Via Giuseppe Moruzzi 1, 56124, Pisa, Italy.

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