Expert opinion on NSCLC small specimen biomarker testing - Part 1: Tissue collection and management.
Best practice
Biopsy
Cytological techniques
Histology
Molecular diagnostics
Non-small cell lung carcinoma
Journal
Virchows Archiv : an international journal of pathology
ISSN: 1432-2307
Titre abrégé: Virchows Arch
Pays: Germany
ID NLM: 9423843
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
received:
16
12
2021
accepted:
18
05
2022
revised:
16
05
2022
pubmed:
21
7
2022
medline:
23
9
2022
entrez:
20
7
2022
Statut:
ppublish
Résumé
Biomarker testing is crucial for treatment selection in advanced non-small cell lung cancer (NSCLC). However, the quantity of available tissue often presents a key constraint for patients with advanced disease, where minimally invasive tissue biopsy typically returns small samples. In Part 1 of this two-part series, we summarise evidence-based recommendations relating to small sample processing for patients with NSCLC. Generally, tissue biopsy techniques that deliver the greatest quantity and quality of tissue with the least risk to the patient should be selected. Rapid on-site evaluation can help to ensure sufficient sample quality and quantity. Sample processing should be managed according to biomarker testing requirements, because tissue fixation methodology influences downstream nucleic acid, protein and morphological analyses. Accordingly, 10% neutral buffered formalin is recommended as an appropriate fixative, and the duration of fixation is recommended not to exceed 24-48 h. Tissue sparing techniques, including the 'one biopsy per block' approach and small sample cutting protocols, can help preserve tissue. Cytological material (formalin-fixed paraffin-embedded [FFPE] cytology blocks and non-FFPE samples such as smears and touch preparations) can be an excellent source of nucleic acid, providing either primary or supplementary patient material to complete morphological and molecular diagnoses. Considerations on biomarker testing, reporting and quality assessment are discussed in Part 2.
Identifiants
pubmed: 35857102
doi: 10.1007/s00428-022-03343-2
pii: 10.1007/s00428-022-03343-2
pmc: PMC9485167
doi:
Substances chimiques
Biomarkers
0
Fixatives
0
Nucleic Acids
0
Formaldehyde
1HG84L3525
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
335-350Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2022. The Author(s).
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