The protein organization of a red blood cell.
Band 3 complex
CP: Cell biology
erythrocytes
protein complexes
proteomics
red blood cells
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
19 07 2022
19 07 2022
Historique:
received:
28
01
2022
revised:
18
04
2022
accepted:
24
06
2022
entrez:
20
7
2022
pubmed:
21
7
2022
medline:
23
7
2022
Statut:
ppublish
Résumé
Red blood cells (RBCs) (erythrocytes) are the simplest primary human cells, lacking nuclei and major organelles and instead employing about a thousand proteins to dynamically control cellular function and morphology in response to physiological cues. In this study, we define a canonical RBC proteome and interactome using quantitative mass spectrometry and machine learning. Our data reveal an RBC interactome dominated by protein homeostasis, redox biology, cytoskeletal dynamics, and carbon metabolism. We validate protein complexes through electron microscopy and chemical crosslinking and, with these data, build 3D structural models of the ankyrin/Band 3/Band 4.2 complex that bridges the spectrin cytoskeleton to the RBC membrane. The model suggests spring-like compression of ankyrin may contribute to the characteristic RBC cell shape and flexibility. Taken together, our study provides an in-depth view of the global protein organization of human RBCs and serves as a comprehensive resource for future research.
Identifiants
pubmed: 35858567
pii: S2211-1247(22)00905-6
doi: 10.1016/j.celrep.2022.111103
pmc: PMC9764456
mid: NIHMS1856933
pii:
doi:
Substances chimiques
Ankyrins
0
Proteome
0
Spectrin
12634-43-4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
111103Subventions
Organisme : NICHD NIH HHS
ID : R00 HD092613
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD085901
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA243004
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM138348
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK110520
Pays : United States
Organisme : NICHD NIH HHS
ID : L40 HD096554
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM122480
Pays : United States
Informations de copyright
Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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