Persistence of MERS-CoV-spike-specific B cells and antibodies after late third immunization with the MVA-MERS-S vaccine.

IgG ELISpot IgG subclasses MERS-CoV MVA-MERS-S immune persistence late booster vaccination long-term immunity memory B cells neutralizing antibodies viral vector vaccine

Journal

Cell reports. Medicine
ISSN: 2666-3791
Titre abrégé: Cell Rep Med
Pays: United States
ID NLM: 101766894

Informations de publication

Date de publication:
19 07 2022
Historique:
received: 08 10 2020
revised: 25 02 2022
accepted: 16 06 2022
entrez: 20 7 2022
pubmed: 21 7 2022
medline: 23 7 2022
Statut: ppublish

Résumé

The Middle East respiratory syndrome (MERS) is a respiratory disease caused by MERS coronavirus (MERS-CoV). In follow up to a phase 1 trial, we perform a longitudinal analysis of immune responses following immunization with the modified vaccinia virus Ankara (MVA)-based vaccine MVA-MERS-S encoding the MERS-CoV-spike protein. Three homologous immunizations were administered on days 0 and 28 with a late booster vaccination at 12 ± 4 months. Antibody isotypes, subclasses, and neutralization capacity as well as T and B cell responses were monitored over a period of 3 years using standard and bead-based enzyme-linked immunosorbent assay (ELISA), 50% plaque-reduction neutralization test (PRNT50), enzyme-linked immunospot (ELISpot), and flow cytometry. The late booster immunization significantly increases the frequency and persistence of spike-specific B cells, binding immunoglobulin G1 (IgG1) and neutralizing antibodies but not T cell responses. Our data highlight the potential of a late boost to enhance long-term antibody and B cell immunity against MERS-CoV. Our findings on the MVA-MERS-S vaccine may be of relevance for coronavirus 2019 (COVID-19) vaccination strategies.

Identifiants

pubmed: 35858586
pii: S2666-3791(22)00221-X
doi: 10.1016/j.xcrm.2022.100685
pmc: PMC9295383
pii:
doi:

Substances chimiques

Antibodies, Viral 0
Viral Vaccines 0

Banques de données

ClinicalTrials.gov
['NCT03615911']

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

100685

Investigateurs

Etienne Bartels (E)
Swantje Gundlach (S)
Thomas Hesterkamp (T)
Verena Krähling (V)
Susan Lassen (S)
My Linh Ly (ML)
Joseph H Pötsch (JH)
Stefan Schmiedel (S)
Asisa Volz (A)
Madeleine E Zinser (ME)

Informations de copyright

Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Références

Lancet Infect Dis. 2020 Jul;20(7):816-826
pubmed: 32325038
Nat Commun. 2014;5:3067
pubmed: 24473083
Clin Transl Immunology. 2021 Feb 28;10(3):e1258
pubmed: 33680466
Nat Commun. 2022 Jul 19;13(1):4182
pubmed: 35853863
Lancet Microbe. 2022 Jan;3(1):e11-e20
pubmed: 34751259
Lancet Infect Dis. 2014 Dec;14(12):1196-207
pubmed: 25455987
EMBO Mol Med. 2022 Mar 7;14(3):e15227
pubmed: 34994081
J Infect Dis. 2018 Jul 13;218(4):614-623
pubmed: 29912453
Lancet Infect Dis. 2019 Sep;19(9):1013-1022
pubmed: 31351922
Lancet HIV. 2020 Apr;7(4):e238-e248
pubmed: 32035516
Lancet. 2021 Dec 18;398(10318):2258-2276
pubmed: 34863358
Clin Infect Dis. 2021 Aug 2;73(3):e550-e558
pubmed: 32898238
Emerg Infect Dis. 2016 Jun;22(6):
pubmed: 27192543
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):9081-6
pubmed: 27457935
Nat Commun. 2021 Apr 1;12(1):2037
pubmed: 33795692
Vaccine. 2006 Jan 30;24(5):572-7
pubmed: 16171909
Lancet Infect Dis. 2014 Jan;14(1):50-56
pubmed: 24239323
J Virol. 2013 Nov;87(21):11950-4
pubmed: 23986586
N Engl J Med. 2012 Nov 8;367(19):1814-20
pubmed: 23075143
Annu Rev Virol. 2016 Sep 29;3(1):237-261
pubmed: 27578435
Cell. 2020 Dec 10;183(6):1508-1519.e12
pubmed: 33207184
Nat Immunol. 2022 Feb;23(2):186-193
pubmed: 35105982
Proc Natl Acad Sci U S A. 2014 Aug 26;111(34):12283-7
pubmed: 25136134
Nat Med. 2016 Jul 7;22(7):701-5
pubmed: 27387881
Immunology. 2018 Mar;153(3):279-289
pubmed: 29139548
Lancet Infect Dis. 2018 Aug;18(8):e217-e227
pubmed: 29680581
Trends Immunol. 2019 Mar;40(3):197-211
pubmed: 30745265
Nat Commun. 2021 Mar 17;12(1):1715
pubmed: 33731724
NPJ Vaccines. 2020 Mar 19;5(1):24
pubmed: 32218996
JCI Insight. 2020 Nov 5;5(21):
pubmed: 33031099
Front Microbiol. 2019 Aug 02;10:1781
pubmed: 31428074
Immunity. 1998 Mar;8(3):363-72
pubmed: 9529153
Immunity. 2010 Oct 29;33(4):451-63
pubmed: 21029957
Lancet. 2021 Jun 12;397(10291):2248
pubmed: 34119059
Lancet Infect Dis. 2020 Jul;20(7):827-838
pubmed: 32325037
Vaccine. 1996 Aug;14(11):1019-27
pubmed: 8879096
Expert Rev Vaccines. 2014 Jun;13(6):761-74
pubmed: 24766432
Nat Med. 2021 Jul;27(7):1205-1211
pubmed: 34002089
J Immunol Methods. 2012 Dec 14;386(1-2):117-23
pubmed: 23023091
Front Immunol. 2019 Oct 18;10:2458
pubmed: 31681331
Front Immunol. 2019 Mar 18;10:440
pubmed: 30949165
Lancet. 2021 Mar 6;397(10277):881-891
pubmed: 33617777
Zoonoses Public Health. 2016 Feb;63(1):1-9
pubmed: 25545147
Vaccine. 2021 Jul 22;39(32):4423-4428
pubmed: 34210573
Clin Microbiol Infect. 2022 Feb;28(2):292-296
pubmed: 34139334
mBio. 2020 Sep 25;11(5):
pubmed: 32978311
BMB Rep. 2019 Jun;52(6):397-402
pubmed: 30355437
Nat Med. 2021 Jun;27(6):1113
pubmed: 33958800
Sci Transl Med. 2014 Mar 19;6(228):228ra38
pubmed: 24648341

Auteurs

Leonie M Weskamm (LM)

Institute for Infection Research and Vaccine Development (IIRVD), University Medical Centre Hamburg-Eppendorf, Hamburg, Germany; Department for Clinical Immunology of Infectious Diseases, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany; German Centre for Infection Research, Hamburg-Lübeck-Borstel-Riems, Germany. Electronic address: m.weskamm@uke.de.

Anahita Fathi (A)

Institute for Infection Research and Vaccine Development (IIRVD), University Medical Centre Hamburg-Eppendorf, Hamburg, Germany; Department for Clinical Immunology of Infectious Diseases, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany; German Centre for Infection Research, Hamburg-Lübeck-Borstel-Riems, Germany; First Department of Medicine, Division of Infectious Diseases, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany.

Matthijs P Raadsen (MP)

Department of Virology, Erasmus Medical Centre, Rotterdam, the Netherlands.

Anna Z Mykytyn (AZ)

Department of Virology, Erasmus Medical Centre, Rotterdam, the Netherlands.

Till Koch (T)

Institute for Infection Research and Vaccine Development (IIRVD), University Medical Centre Hamburg-Eppendorf, Hamburg, Germany; Department for Clinical Immunology of Infectious Diseases, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany; German Centre for Infection Research, Hamburg-Lübeck-Borstel-Riems, Germany; First Department of Medicine, Division of Infectious Diseases, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany.

Michael Spohn (M)

Research Institute Children's Cancer Centre Hamburg, Hamburg, Germany; Department of Pediatric Hematology and Oncology, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany; Bioinformatics Core Unit, Hamburg University Medical Centre, Hamburg, Germany.

Monika Friedrich (M)

Institute for Infection Research and Vaccine Development (IIRVD), University Medical Centre Hamburg-Eppendorf, Hamburg, Germany; Department for Clinical Immunology of Infectious Diseases, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany; German Centre for Infection Research, Hamburg-Lübeck-Borstel-Riems, Germany.

Bart L Haagmans (BL)

Department of Virology, Erasmus Medical Centre, Rotterdam, the Netherlands.

Stephan Becker (S)

German Centre for Infection Research, Gießen-Marburg-Langen, Germany; Institute for Virology, Philipps University Marburg, Marburg, Germany.

Gerd Sutter (G)

German Centre for Infection Research, München, Germany; Division of Virology, Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences, LMU Munich, Munich, Germany.

Christine Dahlke (C)

Institute for Infection Research and Vaccine Development (IIRVD), University Medical Centre Hamburg-Eppendorf, Hamburg, Germany; Department for Clinical Immunology of Infectious Diseases, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany; German Centre for Infection Research, Hamburg-Lübeck-Borstel-Riems, Germany. Electronic address: c.dahlke@uke.de.

Marylyn M Addo (MM)

Institute for Infection Research and Vaccine Development (IIRVD), University Medical Centre Hamburg-Eppendorf, Hamburg, Germany; Department for Clinical Immunology of Infectious Diseases, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany; German Centre for Infection Research, Hamburg-Lübeck-Borstel-Riems, Germany; First Department of Medicine, Division of Infectious Diseases, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female
Humans United States Aged Cross-Sectional Studies Medicare Part C
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH