Improving naive B cell isolation by absence of CD45RB glycosylation and CD27 expression in combination with BCR isotype.
B cell receptor
CD27
CD45RB glycosylation
Naive B cells
somatic hypermutation
Journal
European journal of immunology
ISSN: 1521-4141
Titre abrégé: Eur J Immunol
Pays: Germany
ID NLM: 1273201
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
revised:
06
07
2022
received:
26
05
2022
accepted:
20
07
2022
pubmed:
22
7
2022
medline:
14
10
2022
entrez:
21
7
2022
Statut:
ppublish
Résumé
In past years ex vivo and in vivo experimental approaches involving human naive B cells have proven fundamental for elucidation of mechanisms promoting B cell differentiation in both health and disease. For such studies, it is paramount that isolation strategies yield a population of bona fide naive B cells, i.e., B cells that are phenotypically and functionally naive, clonally non-expanded, and have non-mutated BCR variable regions. In this study different combinations of common as well as recently identified B cell markers were compared to isolate naive B cells from human peripheral blood. High-throughput BCR sequencing was performed to analyze levels of somatic hypermutation and clonal expansion. Additionally, contamination from mature mutated B cells intrinsic to each cell-sorting strategy was evaluated and how this impacts the purity of obtained populations. Our results show that current naive B cell isolation strategies harbor contamination from non-naive B cells, and use of CD27-IgD
Identifiants
pubmed: 35862268
doi: 10.1002/eji.202250013
doi:
Substances chimiques
Immunoglobulin D
0
Immunoglobulin Isotypes
0
Immunoglobulin M
0
Tumor Necrosis Factor Receptor Superfamily, Member 7
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1630-1639Informations de copyright
© 2022 Wiley-VCH GmbH.
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