Experimental borosilicate bioactive glasses: Pulp cells cytocompatibility and mechanical characterization.
al-free glasses
bioactivity
borosilicate glass
cytocompatibility
endodontics
ionomer glass
mechanical properties
pulp cells
restorative dentistry
Journal
International endodontic journal
ISSN: 1365-2591
Titre abrégé: Int Endod J
Pays: England
ID NLM: 8004996
Informations de publication
Date de publication:
Oct 2022
Oct 2022
Historique:
revised:
11
07
2022
received:
13
07
2021
accepted:
12
07
2022
pubmed:
22
7
2022
medline:
11
9
2022
entrez:
21
7
2022
Statut:
ppublish
Résumé
To assess in vitro the effect of two novel phase separated borosilicate glasses (PSBS) in the system SiO The cytocompatibility assessment of the two novel borosilicate glasses, one without alumina (PSBS8) and one containing alumina (PSBS16), was performed on cultured primary human pulp cells. Alamar blue assay was used to assess cell metabolic activity and cell morphology was evaluated by confocal imaging. The bioactivity in Stimulated Body Fluid was also evaluated after 1 and 3 weeks of immersion using SEM-EDX analysis. Vickers microhardness and flexural strength were assessed after incorporating the glass particles into a commercial glass ionomer cement (GIC) liquid containing both polyacrylic and polybasic carboxylic acid. The data revealed that the two borosilicate glasses enhanced cell viability ratios at all-time points in both direct and indirect contact assays. After 3 days of contact, PSBS8 without alumina showed higher viability rate (152%) compared to the PSBS16 containing alumina (145%) and the conventional glass ionomer particles (117%). EDX analysis confirmed an initial Ca/P ratio of 2.1 for 45S5K and 2.08 for PSBS8 without alumina after 3 weeks of immersion. The cement prepared using PSBS8 showed significantly higher Vickers hardness values (p = .001) than that prepared using PSBS16 (46.6 vs. 36.7 MPa). After 24 h of maturation, PSBS8 (without alumina) exhibited a flexural strength of 12.9 MPa compared to a value of 16.4 MPa for the commercial control. PSBS8 without alumina had a higher strength than PSBS16 with alumina, after 1 and 7 days of maturation (p = .001). The present in vitro results demonstrated that the borosilicate bioactive glass without alumina enhanced pulp cell viability, spreading and acellular bioactivity better than the conventional GIC and the experimental borosilicate glass containing alumina.
Substances chimiques
Glass Ionomer Cements
0
Silicon Dioxide
7631-86-9
Aluminum Oxide
LMI26O6933
Types de publication
Journal Article
Langues
eng
Pagination
1053-1065Subventions
Organisme : Seventh Framework Programme
ID : 608197
Informations de copyright
© 2022 British Endodontic Society. Published by John Wiley & Sons Ltd.
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