Erythroblastic islands foster granulopoiesis in parallel to terminal erythropoiesis.
Journal
Blood
ISSN: 1528-0020
Titre abrégé: Blood
Pays: United States
ID NLM: 7603509
Informations de publication
Date de publication:
06 10 2022
06 10 2022
Historique:
accepted:
26
06
2022
received:
28
01
2022
pubmed:
22
7
2022
medline:
12
10
2022
entrez:
21
7
2022
Statut:
ppublish
Résumé
The erythroblastic island (EBI), composed of a central macrophage surrounded by maturing erythroblasts, is the erythroid precursor niche. Despite numerous studies, its precise composition is still unclear. Using multispectral imaging flow cytometry, in vitro island reconstitution, and single-cell RNA sequencing of adult mouse bone marrow (BM) EBI-component cells enriched by gradient sedimentation, we present evidence that the CD11b+ cells present in the EBIs are neutrophil precursors specifically associated with BM EBI macrophages, indicating that erythro-(myelo)-blastic islands are a site for terminal granulopoiesis and erythropoiesis. We further demonstrate that the balance between these dominant and terminal differentiation programs is dynamically regulated within this BM niche by pathophysiological states that favor granulopoiesis during anemia of inflammation and favor erythropoiesis after erythropoietin stimulation. Finally, by molecular profiling, we reveal the heterogeneity of EBI macrophages by cellular indexing of transcriptome and epitope sequencing of mouse BM EBIs at baseline and after erythropoietin stimulation in vivo and provide a searchable online viewer of these data characterizing the macrophage subsets serving as hematopoietic niches. Taken together, our findings demonstrate that EBIs serve a dual role as niches for terminal erythropoiesis and granulopoiesis and the central macrophages adapt to optimize production of red blood cells or neutrophils.
Identifiants
pubmed: 35862735
pii: S0006-4971(22)00923-5
doi: 10.1182/blood.2022015724
pmc: PMC9707396
doi:
Substances chimiques
Epitopes
0
Erythropoietin
11096-26-7
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1621-1634Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR076242
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL122661
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL152099
Pays : United States
Informations de copyright
© 2022 by The American Society of Hematology.
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