A new circular RNA-encoded protein BIRC6-236aa inhibits transmissible gastroenteritis virus (TGEV)-induced mitochondrial dysfunction.
Animals
Calcium
/ metabolism
Cell Line
Peptidyl-Prolyl Isomerase F
/ metabolism
Inhibitor of Apoptosis Proteins
/ genetics
Mitochondria
/ virology
Mitochondrial Permeability Transition Pore
/ metabolism
RNA, Circular
/ genetics
Swine
Transmissible gastroenteritis virus
/ genetics
Voltage-Dependent Anion Channel 1
/ metabolism
BIRC6-236aa
circBIRC6-2
mitochondrial permeability transition pore
transmissible gastroenteritis virus
voltage-dependent anion-selective channel protein 1 (VDAC1)
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
received:
13
10
2021
revised:
04
07
2022
accepted:
06
07
2022
pubmed:
22
7
2022
medline:
30
9
2022
entrez:
21
7
2022
Statut:
ppublish
Résumé
Transmissible gastroenteritis virus (TGEV), a member of the coronavirus family, is the pathogen responsible for transmissible gastroenteritis, which results in mitochondrial dysfunction in host cells. Previously, we identified 123 differentially expressed circular RNAs (cRNA)from the TGEV-infected porcine intestinal epithelial cell line jejunum 2 (IPEC-J2). Previous bioinformatics analysis suggested that, of these, circBIRC6 had the potential to regulate mitochondrial function. Furthermore, mitochondrial permeability transition, a key step in the process of mitochondrial dysfunction, is known to be caused by abnormal opening of mitochondrial permeability transition pores (mPTPs) regulated by the voltage-dependent anion-selective channel protein 1 (VDAC)-Cyclophilin D (CypD) complex. Therefore, in the present study, we investigated the effects of circBIRC6-2 on mitochondrial dysfunction and opening of mPTPs. We found that TGEV infection reduced circBIRC6-2 levels, which in turn reduced mitochondrial calcium (Ca
Identifiants
pubmed: 35863430
pii: S0021-9258(22)00722-0
doi: 10.1016/j.jbc.2022.102280
pmc: PMC9400091
pii:
doi:
Substances chimiques
Peptidyl-Prolyl Isomerase F
0
Inhibitor of Apoptosis Proteins
0
Mitochondrial Permeability Transition Pore
0
RNA, Circular
0
Voltage-Dependent Anion Channel 1
EC 1.6.-
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
102280Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.