[Low-frequency pulsed electromagnetic fields promote osteoblast mineralization and maturation of rats through the PC2/sAC/PKA/CREB signaling pathway].
low-frequency pulsed electromagnetic field
osteoblasts
polycystin2
sAC/PKA/CREB signaling pathway
Journal
Nan fang yi ke da xue xue bao = Journal of Southern Medical University
ISSN: 1673-4254
Titre abrégé: Nan Fang Yi Ke Da Xue Xue Bao
Pays: China
ID NLM: 101266132
Informations de publication
Date de publication:
20 Jul 2022
20 Jul 2022
Historique:
entrez:
23
7
2022
pubmed:
24
7
2022
medline:
27
7
2022
Statut:
ppublish
Résumé
To explore whether the effect of low-frequency pulsed electromagnetic fields (PEMFs) in promoting osteoblast mineralization and maturation is related to the primary cilia, polycystin2 (PC2) and sAC/PKA/CREB signaling pathway. We detected the expression levels of PC2, sAC, PKA, CREB and their phosphorylated proteins in primary rat calvarial osteoblasts exposed to 50 Hz 0.6 mT PEMFs for 0, 5, 15, 30, 60, 90, and 120 min. We blocked PC2 function with amiloride hydrochloride and detected the changes in the activity of sAC/PKA/CREB signal pathway and the mineralization and maturation of the osteoblasts. These examinations were repeated in the osteoblasts after specific knockdown of PC2 via RNA interference and were the co-localization of PC2, sAC, PKA, CREB and their phosphorylated proteins with the primary cilia were using immunofluorescence staining. The expressions of PC2 and the signaling proteins of sAC/PKA/CREB pathway were detected after inhibition of primary ciliation by RNA interference. The expression levels of PC2, sAC, p-PKA and p- CREB were significantly increased in the osteoblasts after exposure to PEMFs for different time lengths ( PC2, located on the surface of the primary cilia of osteoblasts, can perceive and transmit the physical signals from PEMFs and promote the mineralization and maturation of osteoblasts by activating the PC2/ sAC/PKA/CREB signaling pathway.
Identifiants
pubmed: 35869760
doi: 10.12122/j.issn.1673-4254.2022.07.04
pmc: PMC9308874
doi:
Types de publication
Journal Article
Langues
chi
Sous-ensembles de citation
IM
Pagination
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