In vitro growth (IVG) of human ovarian follicles in frozen thawed ovarian cortex tissue culture supplemented with follicular fluid under hypoxic conditions.
Follicle
Ovarian tissue
Reproduction
Journal
Archives of gynecology and obstetrics
ISSN: 1432-0711
Titre abrégé: Arch Gynecol Obstet
Pays: Germany
ID NLM: 8710213
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
received:
26
05
2022
accepted:
14
06
2022
pubmed:
25
7
2022
medline:
16
9
2022
entrez:
24
7
2022
Statut:
ppublish
Résumé
Despite its clinical success rates, transplantation after ovarian tissue cryopreservation (OTC) remains a matter of concern. Certain cancer subtypes may lead to the transfer of malignant cells when transplantation of affected ovarian tissue is conducted. IVG and subsequent isolation of vital follicles obtained from frozen thawed ovarian tissue for further in vitro maturation (IVM) would expand current fertility protection techniques while reducing the risk of retransplanting malignant cells. A total of 216 cortical biopsies from 3 patients were included in this study in 4 treatment groups. After freezing, thawing and 8 days of hypoxic tissue culture supplemented with different concentrations of human follicular fluid (HuFF) and follicle-stimulating hormone (FSH), follicles were isolated enzymatically and stained with calcein to determine follicular viability. Numbers and size of vital follicles were assessed by fluorescence microscopy (Ti2, Nikon) and specified by computer assisted, semi-automated measurement (NIS software, Nikon). To estimate the effect of in vitro culture on apoptosis, tissue sections were stained for nicked DNA (TUNEL) prior and after tissue culture. Analysing 3025 vital follicles, we observed significant differences [P < 0.01] regarding follicle size when hypoxic tissue culture was supplemented with HuFF compared with the control group on day 1, individual follicles reached sizes > 100 µm. The results implicate that HuFF contains valuable factors contributing to significant IVG of follicles in human ovarian tissue and could be regarded as an additional tool in personalized fertility restoration prior to retransplantation of ovarian tissue.
Sections du résumé
BACKGROUND
Despite its clinical success rates, transplantation after ovarian tissue cryopreservation (OTC) remains a matter of concern. Certain cancer subtypes may lead to the transfer of malignant cells when transplantation of affected ovarian tissue is conducted. IVG and subsequent isolation of vital follicles obtained from frozen thawed ovarian tissue for further in vitro maturation (IVM) would expand current fertility protection techniques while reducing the risk of retransplanting malignant cells.
METHODS
A total of 216 cortical biopsies from 3 patients were included in this study in 4 treatment groups. After freezing, thawing and 8 days of hypoxic tissue culture supplemented with different concentrations of human follicular fluid (HuFF) and follicle-stimulating hormone (FSH), follicles were isolated enzymatically and stained with calcein to determine follicular viability. Numbers and size of vital follicles were assessed by fluorescence microscopy (Ti2, Nikon) and specified by computer assisted, semi-automated measurement (NIS software, Nikon). To estimate the effect of in vitro culture on apoptosis, tissue sections were stained for nicked DNA (TUNEL) prior and after tissue culture.
RESULTS
Analysing 3025 vital follicles, we observed significant differences [P < 0.01] regarding follicle size when hypoxic tissue culture was supplemented with HuFF compared with the control group on day 1, individual follicles reached sizes > 100 µm.
CONCLUSIONS
The results implicate that HuFF contains valuable factors contributing to significant IVG of follicles in human ovarian tissue and could be regarded as an additional tool in personalized fertility restoration prior to retransplantation of ovarian tissue.
Identifiants
pubmed: 35871693
doi: 10.1007/s00404-022-06672-4
pii: 10.1007/s00404-022-06672-4
pmc: PMC9470640
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1299-1311Informations de copyright
© 2022. The Author(s).
Références
Br J Cancer. 2006 Apr 10;94(7):1007-10
pubmed: 16570049
Hum Reprod. 2000 Feb;15(2):302-10
pubmed: 10655299
J Ovarian Res. 2018 Jan 5;11(1):4
pubmed: 29304838
Clin Exp Reprod Med. 2012 Sep;39(3):107-13
pubmed: 23106041
Reproduction. 2007 Aug;134(2):253-62
pubmed: 17660235
J Proteome Res. 2017 Aug 4;16(8):3053-3067
pubmed: 28658951
Hum Reprod Update. 2013 Jan-Feb;19(1):67-83
pubmed: 23103636
J Proteome Res. 2008 Jan;7(1):443-9
pubmed: 18047273
Cells Tissues Organs. 2020;209(2-3):75-82
pubmed: 32846416
Proteome Sci. 2016 Nov 14;14:17
pubmed: 27895531
Reprod Biol Endocrinol. 2014 Jul 06;12:60
pubmed: 24997727
N Engl J Med. 2005 Jul 21;353(3):318-21
pubmed: 15983020
Lancet. 2004 Oct 16-22;364(9443):1405-10
pubmed: 15488215
Mol Reprod Dev. 1995 Dec;42(4):447-56
pubmed: 8607975
Int J Mol Sci. 2017 Nov 24;18(12):
pubmed: 29186789
Fertil Steril. 2001 Mar;75(3):588-93
pubmed: 11239546
Tissue Eng Part C Methods. 2009 Sep;15(3):323-32
pubmed: 19552585
Hum Reprod. 2008 May;23(5):1151-8
pubmed: 18326514
Int J Clin Exp Pathol. 2014 Sep 15;7(10):7220-9
pubmed: 25400819
Hum Reprod. 1999 Oct;14(10):2519-24
pubmed: 10527981
Hum Reprod. 1996 Aug;11(8):1668-73
pubmed: 8921114
Reproduction. 2021 Jan;161(1):F33-F40
pubmed: 33361508
Hum Reprod Update. 2013 Jan-Feb;19(1):52-66
pubmed: 23054129
Asian-Australas J Anim Sci. 2012 May;25(5):629-34
pubmed: 25049606
Biol Reprod. 2018 Feb 1;98(2):162-169
pubmed: 29165545
J Proteome Res. 2012 Oct 5;11(10):5090-100
pubmed: 22988950
J Obstet Gynaecol Res. 2013 Feb;39(2):522-7
pubmed: 22925265
Theranostics. 2012;2(7):734-45
pubmed: 22916073
Clin Proteomics. 2020 Jun 08;17:22
pubmed: 32528235
Expert Rev Mol Med. 2016 May 06;18:e9
pubmed: 27149979
Blood. 2010 Oct 21;116(16):2908-14
pubmed: 20595517
Clin Proteomics. 2015 Mar 03;12(1):5
pubmed: 25838815
Science. 2002 Feb 8;295(5557):1009-14
pubmed: 11834815
Hum Reprod. 2013 Jan;28(1):224-9
pubmed: 23019297
Acta Obstet Gynecol Scand. 2016 Sep;95(9):1015-26
pubmed: 27258933
Fertil Steril. 2015 Sep;104(3):672-80.e2
pubmed: 26095134
Reprod Biomed Online. 2019 May;38(5):740-749
pubmed: 30733076
Hum Reprod. 2003 Jun;18(6):1158-64
pubmed: 12773440
Antioxid Redox Signal. 2020 Mar 10;32(8):522-535
pubmed: 31861967
Reprod Biomed Online. 2005 Aug;11(2):162-76
pubmed: 16168210
J Proteomics. 2013 Jul 11;87:68-77
pubmed: 23707233
Acta Obstet Gynecol Scand. 2020 Feb;99(2):213-221
pubmed: 31538662
Fertil Steril. 2016 Jan;105(1):6-12
pubmed: 26674557
Cryobiology. 2007 Dec;55(3):261-8
pubmed: 17931616
CA Cancer J Clin. 2019 Sep;69(5):363-385
pubmed: 31184787
Obstet Gynecol Sci. 2018 Jul;61(4):431-442
pubmed: 30018897
J Clin Oncol. 2018 Oct 5;:JCO1800425
pubmed: 30289731
Hum Reprod. 2009 Sep;24(9):2238-43
pubmed: 19491203
Reprod Biol Endocrinol. 2009 May 04;7:40
pubmed: 19413899
J Assist Reprod Genet. 2017 Mar;34(3):325-336
pubmed: 28028773
Hum Reprod Update. 2010 Jul-Aug;16(4):395-414
pubmed: 20124287
Syst Biol Reprod Med. 2015;61(5):263-76
pubmed: 26114977
Hum Reprod. 1999 Oct;14(10):2525-30
pubmed: 10527982
Transfus Med Hemother. 2019 Jun;46(3):173-181
pubmed: 31244585
J Clin Endocrinol Metab. 2015 Feb;100(2):744-53
pubmed: 25393639
Cancers (Basel). 2021 Jan 08;13(2):
pubmed: 33429908
Eur J Med Res. 2007 Dec 14;12(12):604-8
pubmed: 18024272
N Engl J Med. 2017 Oct 26;377(17):1657-1665
pubmed: 29069558
Hum Reprod. 1994 Apr;9(4):597-603
pubmed: 8046009
Biol Reprod. 2004 Nov;71(5):1730-8
pubmed: 15240420
Hum Reprod. 2016 Sep;31(9):2031-41
pubmed: 27378768
Acta Obstet Gynecol Scand. 2019 May;98(5):653-658
pubmed: 30801653
J Pharm Sci. 1998 Jul;87(7):808-12
pubmed: 9649347
Mol Hum Reprod. 2014 Jan;20(1):31-41
pubmed: 24013158
Reproduction. 2002 Feb;123(2):185-202
pubmed: 11866686
Br J Haematol. 2017 Jul;178(2):231-239
pubmed: 28419412
J Tissue Eng Regen Med. 2019 Oct;13(10):1815-1829
pubmed: 31310055
Fertil Steril. 2010 Nov;94(6):2186-90
pubmed: 20100619
Theriogenology. 1996 Mar;45(4):817-32
pubmed: 16727844
J Proteomics. 2013 Sep 02;90:61-76
pubmed: 23500131
Hum Reprod. 2010 Jul;25(7):1708-12
pubmed: 20472912
Hum Reprod. 2017 May 1;32(5):1046-1054
pubmed: 28333228
Hum Reprod. 2002 May;17(5):1181-8
pubmed: 11980736
Hum Reprod. 2008 May;23(5):1007-13
pubmed: 18344563
J Clin Endocrinol Metab. 2021 Jan 23;106(2):e721-e738
pubmed: 33247906
Hum Reprod. 2006 Nov;21(11):2960-8
pubmed: 16893915
J Assist Reprod Genet. 2015 Mar;32(3):417-27
pubmed: 25595538
Hum Reprod Open. 2020 Nov 14;2020(4):hoaa052
pubmed: 33225079
J Chromatogr A. 2009 Apr 24;1216(17):3621-8
pubmed: 19155017
Hum Reprod. 2002 Jun;17(6):1447-52
pubmed: 12042259
Curr Opin Endocrinol Diabetes Obes. 2008 Dec;15(6):536-47
pubmed: 18971683
Hum Reprod Update. 2013 Sep-Oct;19(5):483-506
pubmed: 23817363
Fertil Steril. 2017 May;107(5):1206-1213
pubmed: 28433369
Biol Reprod. 1996 Jan;54(1):197-207
pubmed: 8838017
Fertil Steril. 2004 Nov;82(5):1390-4
pubmed: 15533365
Fertil Steril. 2011 Aug;96(2):379-383.e3
pubmed: 21719006
Mol Reprod Dev. 2013 Jun;80(6):441-50
pubmed: 23576334
J Clin Pathol. 1980 Aug;33(8):722-9
pubmed: 6933154
Gynecol Oncol. 2003 May;89(2):259-66
pubmed: 12713989
Fertil Steril. 2016 Aug;106(2):467-74
pubmed: 27181924
Lancet. 2015 Feb 7;385(9967):506-7
pubmed: 25705839
Hum Reprod Update. 2018 Jan 1;24(1):15-34
pubmed: 29077897
Mol Hum Reprod. 2018 Mar 1;24(3):135-142
pubmed: 29390119
Eur J Cancer. 2021 Feb;144:310-316
pubmed: 33385947
Sci Rep. 2017 Aug 8;7(1):7533
pubmed: 28790348
Mol Cell Endocrinol. 2015 Feb 5;401:189-201
pubmed: 25528519
J Assist Reprod Genet. 2021 Jun;38(6):1265-1280
pubmed: 34218388