Evidence of impaired mitochondrial cellular bioenergetics in ocular fibroblasts derived from glaucoma patients.
Bioenergetics
Glaucoma
Mitochondria
Oxidative stress
Seahorse XF analyser
Tenon's fibroblast
Journal
Free radical biology & medicine
ISSN: 1873-4596
Titre abrégé: Free Radic Biol Med
Pays: United States
ID NLM: 8709159
Informations de publication
Date de publication:
20 08 2022
20 08 2022
Historique:
received:
17
04
2022
revised:
29
06
2022
accepted:
12
07
2022
pubmed:
26
7
2022
medline:
17
8
2022
entrez:
25
7
2022
Statut:
ppublish
Résumé
Glaucoma is a progressive optic neuropathy characterized by the neurodegeneration of the retinal ganglion cells (RGCs) resulting in irreversible visual impairment and eventual blindness. RGCs are extremely susceptible to mitochondrial compromise due to their marked bioenergetic requirements and morphology. There is increasing interest in therapies targeting mitochondrial health as a method of preventing visual loss in managing glaucoma. The bioenergetic profile of Tenon's ocular fibroblasts from glaucoma patients and controls was investigated using the Seahorse XF24 analyser. Impaired mitochondrial cellular bioenergetics was detected in glaucomatous ocular fibroblasts including basal respiration, maximal respiration and spare capacity. Spare respiratory capacity levels reflect mitochondrial bio-energetic adaptability in response to pathophysiological stress. Basal oxidative stress was elevated in glaucomatous Tenon's ocular fibroblasts and hydrogen peroxide (H
Identifiants
pubmed: 35872337
pii: S0891-5849(22)00483-X
doi: 10.1016/j.freeradbiomed.2022.07.009
pii:
doi:
Substances chimiques
Hydrogen Peroxide
BBX060AN9V
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
102-110Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.