Sera from Patients with Malignant Pleural Mesothelioma Tested Positive for IgG Antibodies against SV40 Large T Antigen: The Viral Oncoprotein.


Journal

Journal of oncology
ISSN: 1687-8450
Titre abrégé: J Oncol
Pays: Egypt
ID NLM: 101496537

Informations de publication

Date de publication:
2022
Historique:
received: 30 08 2021
revised: 27 01 2022
accepted: 11 06 2022
entrez: 25 7 2022
pubmed: 26 7 2022
medline: 26 7 2022
Statut: epublish

Résumé

Malignant pleural mesothelioma (MPM), a fatal tumor, is mainly linked to the asbestos exposure. It has been reported that together with the inhalation of asbestos fibers, other factors are involved in the MPM onset, including simian virus 40 (SV40). SV40, a polyomavirus with oncogenic potential, induces (i) in vitro the mesenchymal cell transformation, whereas (ii) in vivo the MPM onset in experimental animals. The association between MPM and SV40 in humans remains to be elucidated. Sera (n = 415) from MPM-affected patients (MPM cohort 1; n = 152) and healthy subjects (HSs, n = 263) were investigated for their immunoglobulin G (IgG) against simian virus 40 large tumor antigen (Tag), which is the transforming protein. Sera were investigated with an indirect enzyme-linked immunosorbent assay (ELISA) using two synthetic peptides from SV40 Tag protein. SV40 Tag protein was evaluated by immunohistochemical (IHC) staining on MPM samples (MPM cohort 2; n = 20). Formalin-fixed and paraffin-embedded (FFPE) samples were obtained from MPM patients unrelated to MPM serum donors. The proportion of sera, from MPM patients, showing antibodies against SV40 Tag (34%) was significantly higher compared to HSs (20%) (odds ratio 2.049, CI 95% 1.32-3.224;

Identifiants

pubmed: 35874636
doi: 10.1155/2022/7249912
pmc: PMC9307391
doi:

Types de publication

Journal Article

Langues

eng

Pagination

7249912

Informations de copyright

Copyright © 2022 Elisa Mazzoni et al.

Déclaration de conflit d'intérêts

The authors report no conflicts of interest for this work.

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Auteurs

Elisa Mazzoni (E)

Department of Chemical, Pharmaceutical and Agricultural Sciences-DOCPAS, University of Ferrara, Ferrara 44121, Italy.

Ilaria Bononi (I)

Department of Translational Medicine and for Romagna, University of Ferrara, Ferrara 44121, Italy.

John Charles Rotondo (JC)

Department of Medical Sciences, Section of Experimental Medicine, School of Medicine, University of Ferrara, Ferrara 44121, Italy.

Chiara Mazziotta (C)

Department of Medical Sciences, Section of Experimental Medicine, School of Medicine, University of Ferrara, Ferrara 44121, Italy.

Roberta Libener (R)

Mesothelioma BioBank, Pathology Unit, City Hospital, Alessandria, Italy.

Roberto Guaschino (R)

Transfusion Medicine, City Hospital, Alessandria, Italy.

Roberta Gafà (R)

Section of Pathology, Department of Translational Medicine, School of Medicine, University of Ferrara, Ferrara, Italy.

Giovanni Lanza (G)

Section of Pathology, Department of Translational Medicine, School of Medicine, University of Ferrara, Ferrara, Italy.

Fernanda Martini (F)

Department of Medical Sciences, Section of Experimental Medicine, School of Medicine, University of Ferrara, Ferrara 44121, Italy.
Laboratory for Technologies of Advanced Therapies, Department of Medical Sciences, University of Ferrara, Ferrara 44121, Italy.

Mauro Tognon (M)

Department of Medical Sciences, Section of Experimental Medicine, School of Medicine, University of Ferrara, Ferrara 44121, Italy.

Classifications MeSH