Nanodiamond-Quantum Sensors Reveal Temperature Variation Associated to Hippocampal Neurons Firing.
ODMR
intracellular nanoscale sensing
nanodiamonds
nitrogen-vacancy (NV) centers
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
revised:
28
06
2022
received:
30
05
2022
pubmed:
26
7
2022
medline:
12
10
2022
entrez:
25
7
2022
Statut:
ppublish
Résumé
Temperature is one of the most relevant parameters for the regulation of intracellular processes. Measuring localized subcellular temperature gradients is fundamental for a deeper understanding of cell function, such as the genesis of action potentials, and cell metabolism. Notwithstanding several proposed techniques, at the moment detection of temperature fluctuations at the subcellular level still represents an ongoing challenge. Here, for the first time, temperature variations (1 °C) associated with potentiation and inhibition of neuronal firing is detected, by exploiting a nanoscale thermometer based on optically detected magnetic resonance in nanodiamonds. The results demonstrate that nitrogen-vacancy centers in nanodiamonds provide a tool for assessing various levels of neuronal spiking activity, since they are suitable for monitoring different temperature variations, respectively, associated with the spontaneous firing of hippocampal neurons, the disinhibition of GABAergic transmission and the silencing of the network. Conjugated with the high sensitivity of this technique (in perspective sensitive to < 0.1 °C variations), nanodiamonds pave the way to a systematic study of the generation of localized temperature gradients under physiological and pathological conditions. Furthermore, they prompt further studies explaining in detail the physiological mechanism originating this effect.
Identifiants
pubmed: 35876403
doi: 10.1002/advs.202202014
pmc: PMC9534962
doi:
Substances chimiques
Nanodiamonds
0
Nitrogen
N762921K75
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2202014Subventions
Organisme : EMPIR program
ID : 20IND05QADeT
Organisme : European Union's Horizon 2020 research and innovation program
Organisme : PATHOS EU H2020 FET-OPEN
ID : 828946
Organisme : European Regional Development Fund
ID : CZ.02.1.01/0.0/0.0/16_026/0008382
Organisme : Compagnia di San Paolo
Organisme : PROGETTO TRAPEZIO
ID : 828946
Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2022 The Authors. Advanced Science published by Wiley-VCH GmbH.
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