Dispensability of Ascorbic Acid Uptake and Utilization Encoded by ulaABCD for the Virulence of Haemophilus ducreyi in Humans.
Haemophilus ducreyi
abscess
ascorbic acid
human challenge model
skin infection
Journal
The Journal of infectious diseases
ISSN: 1537-6613
Titre abrégé: J Infect Dis
Pays: United States
ID NLM: 0413675
Informations de publication
Date de publication:
01 02 2023
01 02 2023
Historique:
received:
08
06
2022
accepted:
21
07
2022
pubmed:
26
7
2022
medline:
4
2
2023
entrez:
25
7
2022
Statut:
ppublish
Résumé
Compared with wounded skin, ascorbic acid is enriched in pustules of humans experimentally infected with Haemophilus ducreyi. Compared with the broth-grown inocula, transcription of the H. ducreyi ulaABCD operon, which encodes genes for ascorbic acid uptake, is increased in pustules. We hypothesized that ascorbic acid uptake plays a role in H. ducreyi virulence. Five volunteers were infected with both H. ducreyi strain 35000HP and its isogenic ulaABCD deletion mutant at multiple sites; the papule and pustule formation rates of the mutant and parent strains were similar. Thus, ascorbic acid uptake is not essential for H. ducreyi virulence in humans.
Identifiants
pubmed: 35876728
pii: 6649666
doi: 10.1093/infdis/jiac314
pmc: PMC10169391
doi:
Substances chimiques
Ascorbic Acid
PQ6CK8PD0R
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
317-321Subventions
Organisme : NIH HHS
ID : UL1TR002529
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR002529
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007637
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI137116
Pays : United States
Organisme : NIAID NIH HHS
ID : T32AI007637
Pays : United States
Informations de copyright
The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Références
Infect Immun. 2003 Dec;71(12):7178-82
pubmed: 14638812
J Bacteriol. 2022 May 17;204(5):e0000522
pubmed: 35377183
Microbiology (Reading). 2007 Oct;153(Pt 10):3399-3408
pubmed: 17906139
J Biol Chem. 1995 May 26;270(21):12584-92
pubmed: 7759506
J Infect Dis. 2009 Jun 1;199(11):1671-9
pubmed: 19432549
J Bacteriol. 2008 Oct;190(20):6615-24
pubmed: 18708499
J Bacteriol. 2002 Jan;184(1):302-6
pubmed: 11741871
J Infect Dis. 1998 Dec;178(6):1684-7
pubmed: 9815220
Front Immunol. 2021 Feb 03;11:615402
pubmed: 33613541
mBio. 2019 Jun 18;10(3):
pubmed: 31213562
J Biol Chem. 1993 Jul 25;268(21):15531-5
pubmed: 8340380
Infect Immun. 2016 Apr 22;84(5):1514-1525
pubmed: 26930707
J Bacteriol. 2004 Mar;186(6):1720-8
pubmed: 14996803
Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13816-9
pubmed: 9391110