Characteristics and in vitro anti skin aging activity and UV radiation protection of morin loaded in niosomes.
drug delivery, melatonin
morin
niosome formulation
skin physiology
Journal
Journal of cosmetic dermatology
ISSN: 1473-2165
Titre abrégé: J Cosmet Dermatol
Pays: England
ID NLM: 101130964
Informations de publication
Date de publication:
Nov 2022
Nov 2022
Historique:
revised:
04
07
2022
received:
07
06
2022
accepted:
22
07
2022
pubmed:
26
7
2022
medline:
28
12
2022
entrez:
25
7
2022
Statut:
ppublish
Résumé
One of the dermatologic problems in elderly people is skin aging, which is a natural and complex biological process. Morin is a flavonoid with high radical scavenging activity as well as antityrosinase effects but its low water solubility has restricted its application. This research aimed to develop, characterize, and optimize morin niosomes composed of non-ionic surfactants, and evaluate the in vitro UV protection and antiaging effectiveness. Niosomes were prepared by the film hydration method with sorbitan monostearate (Span® 40), polyoxyethylenesorbitan monopalmitate (Tween® 40), and cholesterol. The niosomes were characterized in terms of size, zeta potential, morphology, in vitro release behavior, and drug entrapment efficiency (EE). Afterward, antiaging activity, including antityrosinase, antioxidant, intracellular reactive oxygen species (ROS) scavenging, and sun protection factor (SPF) were evaluated. The optimized niosomes appeared as unilamellar vesicles with a spherical shape, with size, zeta potential, and EE values of 6.13 ± 0.40 μm, -0.81 ± 0.32 mV, and 89.35% ± 2.80%, respectively. The noisome formulation remained stable at -4°C for 3 months. The release profiles of morin loaded in niosomes revealed the extended release over 8 h and followed zero-order release kinetics. Morin-loaded niosomes exhibited no significant toxicity toward the L929 cell line. The niosome loaded with morin showed anti skin aging activity, including antityrosinase effects (IC50 = 13.17 ± 1.58 μg/ml), antioxidant (IC50 = 28.49 ± 2.05 μg/ml), and ROS scavenging activity. For 1% and 5% (w/w) morin niosomes in eucerin base cream, the SPF was 39.03 ± 1.01 and 38.15 ± 0.82, respectively, whereas the noisome-free morin cream exhibited an SPF of 4.47 ± 0.56. Morin-loaded niosome has been shown to provide sun protection and antiaging effects, suggesting that it could be used in pharmaceutical and cosmetic products.
Sections du résumé
BACKGROUND
BACKGROUND
One of the dermatologic problems in elderly people is skin aging, which is a natural and complex biological process. Morin is a flavonoid with high radical scavenging activity as well as antityrosinase effects but its low water solubility has restricted its application.
AIMS
OBJECTIVE
This research aimed to develop, characterize, and optimize morin niosomes composed of non-ionic surfactants, and evaluate the in vitro UV protection and antiaging effectiveness.
METHODS
METHODS
Niosomes were prepared by the film hydration method with sorbitan monostearate (Span® 40), polyoxyethylenesorbitan monopalmitate (Tween® 40), and cholesterol. The niosomes were characterized in terms of size, zeta potential, morphology, in vitro release behavior, and drug entrapment efficiency (EE). Afterward, antiaging activity, including antityrosinase, antioxidant, intracellular reactive oxygen species (ROS) scavenging, and sun protection factor (SPF) were evaluated.
RESULTS
RESULTS
The optimized niosomes appeared as unilamellar vesicles with a spherical shape, with size, zeta potential, and EE values of 6.13 ± 0.40 μm, -0.81 ± 0.32 mV, and 89.35% ± 2.80%, respectively. The noisome formulation remained stable at -4°C for 3 months. The release profiles of morin loaded in niosomes revealed the extended release over 8 h and followed zero-order release kinetics. Morin-loaded niosomes exhibited no significant toxicity toward the L929 cell line. The niosome loaded with morin showed anti skin aging activity, including antityrosinase effects (IC50 = 13.17 ± 1.58 μg/ml), antioxidant (IC50 = 28.49 ± 2.05 μg/ml), and ROS scavenging activity. For 1% and 5% (w/w) morin niosomes in eucerin base cream, the SPF was 39.03 ± 1.01 and 38.15 ± 0.82, respectively, whereas the noisome-free morin cream exhibited an SPF of 4.47 ± 0.56.
CONCLUSION
CONCLUSIONS
Morin-loaded niosome has been shown to provide sun protection and antiaging effects, suggesting that it could be used in pharmaceutical and cosmetic products.
Substances chimiques
Liposomes
0
Antioxidants
0
morin
8NFQ3F76WR
Reactive Oxygen Species
0
Flavonoids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6326-6335Subventions
Organisme : Kerman university of medical sciences
ID : 99000411
Informations de copyright
© 2022 Wiley Periodicals LLC.
Références
Lourith N, KaNLayavattaNaKuL M, ChaiKuL P, ChaNsriNiyoM C, BuNwatCharaPhaNsaKuN P. In vitro and cellular activities of the selected fruits residues for skin aging treatment. An Acad Bras Ciênc. 2017;89:577-589.
Chaikul P, Khat-Udomkiri N, Iangthanarat K, Manosroi J, Manosroi A. Characteristics and in vitro anti-skin aging activity of gallic acid loaded in cationic CTAB niosome. Eur J Pharm Sci. 2019;131:39-49.
Acosta JLS, Santoyo FM, Moreno JMF, et al. Study of skin aging effects induced by near UV radiation using time average digital holographic interferometry. Opt Lasers Eng. 2021;137:106345.
Sarkar S, Gaddameedhi S. Solar ultraviolet-induced DNA damage response: melanocytes story in transformation to environmental melanomagenesis. Environ Mol Mutagen. 2020;61(7):736-751.
Matsumura Y, Ananthaswamy HN. Toxic effects of ultraviolet radiation on the skin. Toxicol Appl Pharmacol. 2004;195(3):298-308.
Kaurinovic B, Vastag D. Flavonoids and Phenolic Acids as Potential Natural Antioxidants. IntechOpen; 2019:1-20.
Karami-Mohajeri S, Najafi A, Behnam B, et al. Protective effect of Zataria multiflora Boiss. and its main compound, rosmarinic acid, against malathion induced oxidative stress and apoptosis in HepG2 cells. J Environ Sci B. 2021;56(4):297-306.
Mendoza-Wilson AM, Santacruz-Ortega H, Balandrán-Quintana RR. Relationship between structure, properties, and the radical scavenging activity of morin. J Mol Struct. 2011;995(1-3):134-141.
Khamchai S, Chumboatong W, Hata J, Tocharus C, Suksamrarn A, Tocharus J. Morin protects the blood-brain barrier integrity against cerebral ischemia reperfusion through anti-inflammatory actions in rats. Sci Rep. 2020;10(1):1-13.
Ding X, Yin C, Zhang W, et al. Designing aptamer-gold nanoparticle-loaded pH-sensitive liposomes encapsulate morin for treating cancer. Nanoscale Res Lett. 2020;15:1-17.
Wang Y, Zhang G, Yan J, Gong D. Inhibitory effect of morin on tyrosinase: Insights from spectroscopic and molecular docking studies. Food Chem. 2014;163:226-233.
Shetty PK, Venuvanka V, Jagani HV, et al. Development and evaluation of sunscreen creams containing morin-encapsulated nanoparticles for enhanced UV radiation protection and antioxidant activity. Int J Nanomedicine. 2015;10:6477.
Yassa N, Sharififar F, Shafiee A. Otostegia persica. as a Source of Natural Antioxidants. J Pharm Biol. 2005;43(1):33-38.
Yong HJ, Ahn JJ. Antioxidant and skin protection effect of morin upon UVA exposure. J Dermatol. 2018;2(1):1-7.
Waddad AY, Abbad S, Yu F, et al. Formulation, characterization and pharmacokinetics of Morin hydrate niosomes prepared from various non-ionic surfactants. Int J Pharm. 2013;456(2):446-458.
Choi YA, Yoon YH, Choi K, et al. Enhanced oral bioavailability of morin administered in mixed micelle formulation with PluronicF127 and Tween80 in rats. Biol Pharm Bull. 2015;38(2):208-217.
Lu B, Huang Y, Chen Z, et al. Niosomal nanocarriers for enhanced skin delivery of quercetin with functions of anti-tyrosinase and antioxidant. Molecules. 2019;24(12):2322.
dos Santos LB, de Alcântara CC, da Silva Santos ACR, et al. Development of morin/hydroxypropyl-β-cyclodextrin inclusion complex: Enhancement of bioavailability, antihyperalgesic and anti-inflammatory effects. Food Chem Toxicol. 2019;126:15-24.
Joshi H, Hegde AR, Shetty PK, et al. Sunscreen creams containing naringenin nanoparticles: formulation development and in vitro and in vivo evaluations. Photodermatol Photoimmunol Photomed. 2018;34(1):69-81.
Gollavilli H, Hegde AR, Managuli RS, et al. Naringin nano-ethosomal novel sunscreen creams: development and performance evaluation. Colloids Surf B Biointerfaces. 2020;193:111122.
Avadhani KS, Manikkath J, Tiwari M, et al. Skin delivery of epigallocatechin-3-gallate (EGCG) and hyaluronic acid loaded nano-transfersomes for antioxidant and anti-aging effects in UV radiation induced skin damage. Drug Deliv Lett. 2017;24(1):61-74.
Rameshk M, Sharififar F, Mehrabani M, Pardakhty A, Farsinejad A, Mehrabani M. Proliferation and in vitro wound healing effects of the Microniosomes containing Narcissus tazetta L. bulb extract on primary human fibroblasts (HDFs). Daru. 2018;26(1):31-42.
Harle HD, Ingram JA, Leber PA, Hess KR, Yoder CH. A simple method for determination of solubility in the first-year laboratory. J Chem Educ. 2003;80(5):560.
Pardakhty A, Shakibaie M, Daneshvar H, Khamesipour A, Mohammadi-Khorsand T, Forootanfar H. Preparation and evaluation of niosomes containing autoclaved Leishmania major: a preliminary study. J Microencapsul. 2012;29(3):219-224.
Raeiszadeh M, Pardakhty A, Sharififar F, et al. Development, physicochemical characterization, and antimicrobial evaluation of niosomal myrtle essential oil. Res Pharm Sci. 2018;13(3):250-261.
Sharififar F, Moshafi M, Shafazand E, Koohpayeh A. Acetyl cholinesterase inhibitory, antioxidant and cytotoxic activity of three dietary medicinal plants. J Food Chem. 2012;130(1):20-23.
Jamshidzadeh A, Shokri Y, Ahmadi N, Mohamadi N, Sharififar F. Quercus infectoria and Terminalia chebula decrease melanin content and tyrosinase activity in B16/F10 cell lines. J Pharm Pharmacogn Res. 2017;5(5):270-277.
Gharavi SM, Tavakoli N, Pardakhti A, Baghaizadeh N. Determination of sun protection factor of sunscreens by two different in-vitro methods. J Res Med Sci. 2000;5(2):48-53.
Khazaeli P, Mehrabani M. Screening of sun protective activity of the ethyl acetate extracts of some medicinal plants. Iran J Pharm Sci. 2010;1:5-9.
Sayre RM, Agin P, Levee G, Marlowe E. Comparison of in vivo and in vitro testing of sunscreening formulas. Photochem Photobiol. 1979;29:559-566.
Mokhtar M, Sammour OA, Hammad MA, Megrab NA. Effect of some formulation parameters on flurbiprofen encapsulation and release rates of niosomes prepared from proniosomes. Int J Pharm. 2008;361(1-2):104-111.
Kumar R. Lipid-Based Nanoparticles for Drug-Delivery systems Nanocarriers for Drug Delivery. Elsevier; 2019:249-284.
Shin S, Ko J, Kim M, Song N, Park K. Morin induces melanogenesis via activation of MAPK signaling pathways in B16F10 mouse melanoma cells. Molecules. 2021;26(8):2150.
Kim D, Park J, Kim J, et al. Flavonoids as mushroom tyrosinase inhibitors: a fluorescence quenching study. J Agric Food Chem. 2006;54(3):935-941.
Zeng L-H, Wu J, Fung B, Tong JH, Mickle D, Wu T-W. Comparative protection against oxyradicals by three flavonoids on cultured endothelial cells. Biochem Cell Biol. 1997;75(6):717-720.
Chang L-W, Juang L-J, Wang B-S, et al. Antioxidant and antityrosinase activity of mulberry (Morus alba L.) twigs and root bark. Food Chem Toxicol. 2011;49(4):785-790.
Shanbhag S, Nayak A, Narayan R, Nayak UY. Anti-aging and sunscreens: paradigm shift in cosmetics. Adv Pharm Bull. 2019;9(3):348-359.
Dutra EA, Kedor-Hackmann ERM, Santoro MIRM. Determination of sun protection factor (SPF) of sunscreens by ultraviolet spectrophotometry. Rev Bras Cienc Farm. 2004;40(3):381-385.
Kimura E, Kawano Y, Todo H, Ikarashi Y, Sugibayashi K. Measurement of skin permeation/penetration of nanoparticles for their safety evaluation. Biol Pharm Bull. 2012;35(9):1476-1486.