Optimized intramuscular immunization with VSV-vectored spike protein triggers a superior immune response to SARS-CoV-2.


Journal

NPJ vaccines
ISSN: 2059-0105
Titre abrégé: NPJ Vaccines
Pays: England
ID NLM: 101699863

Informations de publication

Date de publication:
25 Jul 2022
Historique:
received: 16 09 2021
accepted: 21 06 2022
entrez: 25 7 2022
pubmed: 26 7 2022
medline: 26 7 2022
Statut: epublish

Résumé

Immunization with vesicular stomatitis virus (VSV)-vectored COVID-19 vaccine candidates expressing the SARS-CoV-2 spike protein in place of the VSV glycoprotein relies implicitly on expression of the ACE2 receptor at the muscular injection site. Here, we report that such a viral vector vaccine did not induce protective immunity following intramuscular immunization of K18-hACE2 transgenic mice. However, when the viral vector was trans-complemented with the VSV glycoprotein, intramuscular immunization resulted in high titers of spike-specific neutralizing antibodies. The vaccinated animals were fully protected following infection with a lethal dose of SARS-CoV-2-S

Identifiants

pubmed: 35879345
doi: 10.1038/s41541-022-00508-7
pii: 10.1038/s41541-022-00508-7
pmc: PMC9309237
doi:

Types de publication

Journal Article

Langues

eng

Pagination

82

Subventions

Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation)
ID : 310030_189151
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation)
ID : 4078P0_198473
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation)
ID : 31CA30_196062

Informations de copyright

© 2022. The Author(s).

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Auteurs

Adriano Taddeo (A)

Institute of Virology and Immunology (IVI), Mittelhäusern and Bern, Bern, Switzerland.
Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

Inês Berenguer Veiga (IB)

Institute of Virology and Immunology (IVI), Mittelhäusern and Bern, Bern, Switzerland.
Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

Christelle Devisme (C)

Institute of Virology and Immunology (IVI), Mittelhäusern and Bern, Bern, Switzerland.
Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

Renate Boss (R)

Federal Food Safety and Veterinary Office, Bern, Switzerland.

Philippe Plattet (P)

Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

Sebastian Weigang (S)

Institute of Virology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

Georg Kochs (G)

Institute of Virology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

Volker Thiel (V)

Institute of Virology and Immunology (IVI), Mittelhäusern and Bern, Bern, Switzerland.
Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

Charaf Benarafa (C)

Institute of Virology and Immunology (IVI), Mittelhäusern and Bern, Bern, Switzerland. charaf.benarafa@ivi.admin.ch.
Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland. charaf.benarafa@ivi.admin.ch.

Gert Zimmer (G)

Institute of Virology and Immunology (IVI), Mittelhäusern and Bern, Bern, Switzerland. gert.zimmer@ivi.admin.ch.
Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland. gert.zimmer@ivi.admin.ch.

Classifications MeSH