Cytoprotective effects and antioxidant activities of acteoside and various extracts of Clerodendrum cyrtophyllum Turcz leaves against t-BHP induced oxidative damage.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
25 07 2022
25 07 2022
Historique:
received:
14
02
2022
accepted:
20
07
2022
entrez:
25
7
2022
pubmed:
26
7
2022
medline:
28
7
2022
Statut:
epublish
Résumé
This study evaluates the antioxidant potential and cytoprotective effects of ethanolic crude extract from Clerodendrum cyrtophyllum leaves (ECE) and five derived fractions (namely, petroleum ether fraction (PEF), dichloromethane fraction (DMF), ethyl acetate fraction (EAF), n-butyl alcohol fraction (BAF) and the remaining fraction (RF)), as well as acteoside (Ac, a major phenolic component in EAF) on oxidative damage caused by tert-butyl hydroperoxide (t-BHP) in HepG2 cells. MTT assay results showed that ECE, EAF, BAF, RF and Ac increased the viability of t-BHP-damaged cells in a dose-dependent manner, while EAF significantly promoted cell viability. EAF, BAF, RF, or Ac reduced the levels of lactate dehydrogenase (LDH) leakage, malondialdehyde (MDA), and reactive oxygen species (ROS). Additionally, glutathione (GSH) levels and the activities of superoxide dismutase (SOD) and catalase (CAT) increased. Western blot analysis further indicated that EAF, BAF, RF, or Ac up-regulated pro-caspase-3 and reduced cleaved caspase-3 during t-BHP-induced oxidative stress. Flow cytometry analysis and fluorescence micrographs showed that Ac could inhibit apoptosis.
Identifiants
pubmed: 35879416
doi: 10.1038/s41598-022-17038-w
pii: 10.1038/s41598-022-17038-w
pmc: PMC9314432
doi:
Substances chimiques
Antioxidants
0
Glucosides
0
Phenols
0
Plant Extracts
0
Reactive Oxygen Species
0
acteoside
3TGX09BD5B
tert-Butylhydroperoxide
955VYL842B
Glutathione
GAN16C9B8O
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
12630Informations de copyright
© 2022. The Author(s).
Références
Eur J Pharmacol. 2002 Sep 13;451(2):119-24
pubmed: 12231380
Free Radic Biol Med. 2020 Jan;146:79-91
pubmed: 31634539
Synth Syst Biotechnol. 2016 Dec 24;2(1):13-22
pubmed: 29062957
Redox Biol. 2018 Jun;16:169-178
pubmed: 29505920
Food Res Int. 2016 Dec;90:33-41
pubmed: 29195889
Food Chem. 2018 Jul 1;253:5-12
pubmed: 29502843
Food Chem Toxicol. 2013 Sep;59:554-63
pubmed: 23838314
Cell Biochem Biophys. 2009;53(2):75-100
pubmed: 19184542
Life Sci. 2004 Jan 9;74(8):1051-64
pubmed: 14672760
Eur J Med Chem. 2019 Sep 15;178:687-704
pubmed: 31228811
Toxicology. 2013 Apr 5;306:24-34
pubmed: 23410634
Exp Gerontol. 2017 Jan;87(Pt A):23-32
pubmed: 27887985
Oxid Med Cell Longev. 2014;2014:310504
pubmed: 25013541
Semin Cell Dev Biol. 2018 Aug;80:50-64
pubmed: 28587975
J Asian Nat Prod Res. 2011 May;13(5):449-64
pubmed: 21534045
J Pharm Pharmacol. 2004 Jun;56(6):743-8
pubmed: 15231039
Free Radic Biol Med. 2013 Jul;60:1-4
pubmed: 23434764
J Tradit Complement Med. 2017 May 19;8(1):120-127
pubmed: 29321999
PLoS One. 2013 Jul 16;8(7):e68392
pubmed: 23874607
J Ethnopharmacol. 2018 Oct 28;225:18-30
pubmed: 29935346
PLoS One. 2020 Jun 23;15(6):e0234435
pubmed: 32574221
J Nat Prod. 2001 Jul;64(7):915-9
pubmed: 11473423
Food Chem. 2016 Apr 1;196:885-96
pubmed: 26593569
Food Chem. 2017 Dec 15;237:912-920
pubmed: 28764086
Food Chem. 2013 Jan 1;136(1):9-17
pubmed: 23017386
Biochem Biophys Res Commun. 2017 Jan 15;482(3):419-425
pubmed: 28212725
Cell Biol Int. 2006 Nov;30(11):924-32
pubmed: 16895761
PeerJ. 2016 Jan 28;4:e1628
pubmed: 26839752
Mutat Res. 2013 Sep 18;757(1):15-22
pubmed: 23867853