Adaptive responses of carbon and nitrogen metabolisms to nitrogen-deficiency in Citrus sinensis seedlings.
Amino acids
Carbon metabolism
Citrus sinensis
Nitrogen metabolism
Nitrogen-deficiency
Organic acids
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
26 Jul 2022
26 Jul 2022
Historique:
received:
15
03
2022
accepted:
11
07
2022
entrez:
25
7
2022
pubmed:
26
7
2022
medline:
28
7
2022
Statut:
epublish
Résumé
In China, nitrogen (N)-deficiency often occurs in Citrus orchards, which is one of the main causes of yield loss and fruit quality decline. Little information is known about the adaptive responses of Citrus carbon (C) and N metabolisms to N-deficiency. Seedlings of 'Xuegan' (Citrus sinensis (L.) Osbeck) were supplied with nutrient solution at an N concentration of 0 (N-deficiency), 5, 10, 15 or 20 mM for 10 weeks. Thereafter, we examined the effects of N supply on the levels of C and N in roots, stems and leaves, and the levels of organic acids, nonstructural carbohydrates, NH N-deficiency elevated sucrose export from leaves to roots, C and N distributions in roots and C/N ratio in roots, stems and leaves, thus enhancing root dry weight/shoot dry weight ratio and N use efficiency. N-deficient leaves displayed decreased accumulation of starch and total nonstructural carbohydrates (TNC) and increased sucrose/starch ratio as well as a partitioning trend of assimilated C toward to sucrose, but N-deficient roots displayed elevated accumulation of starch and TNC and reduced sucrose/starch ratio as well as a partitioning trend of assimilated C toward to starch. N-deficiency reduced the concentrations of most FAADs and the ratios of total FAADs (TFAADs)/N in leaves and roots. N-deficiency reduced the demand for C skeleton precursors for amino acid biosynthesis, thus lowering TFAADs/C ratio in leaves and roots. N-deficiency increased (decreased) the relative amounts of C-rich (N-rich) FAADs, thus increasing the molar ratio of C/N in TFAADs in leaves and roots. Our findings corroborated our hypothesis that C and N metabolisms displayed adaptive responses to N-deficiency in C. sinensis seedlings, and that some differences existed between roots and leaves in N-deficiency-induced alterations of and C and N metabolisms.
Sections du résumé
BACKGROUND
BACKGROUND
In China, nitrogen (N)-deficiency often occurs in Citrus orchards, which is one of the main causes of yield loss and fruit quality decline. Little information is known about the adaptive responses of Citrus carbon (C) and N metabolisms to N-deficiency. Seedlings of 'Xuegan' (Citrus sinensis (L.) Osbeck) were supplied with nutrient solution at an N concentration of 0 (N-deficiency), 5, 10, 15 or 20 mM for 10 weeks. Thereafter, we examined the effects of N supply on the levels of C and N in roots, stems and leaves, and the levels of organic acids, nonstructural carbohydrates, NH
RESULTS
RESULTS
N-deficiency elevated sucrose export from leaves to roots, C and N distributions in roots and C/N ratio in roots, stems and leaves, thus enhancing root dry weight/shoot dry weight ratio and N use efficiency. N-deficient leaves displayed decreased accumulation of starch and total nonstructural carbohydrates (TNC) and increased sucrose/starch ratio as well as a partitioning trend of assimilated C toward to sucrose, but N-deficient roots displayed elevated accumulation of starch and TNC and reduced sucrose/starch ratio as well as a partitioning trend of assimilated C toward to starch. N-deficiency reduced the concentrations of most FAADs and the ratios of total FAADs (TFAADs)/N in leaves and roots. N-deficiency reduced the demand for C skeleton precursors for amino acid biosynthesis, thus lowering TFAADs/C ratio in leaves and roots. N-deficiency increased (decreased) the relative amounts of C-rich (N-rich) FAADs, thus increasing the molar ratio of C/N in TFAADs in leaves and roots.
CONCLUSIONS
CONCLUSIONS
Our findings corroborated our hypothesis that C and N metabolisms displayed adaptive responses to N-deficiency in C. sinensis seedlings, and that some differences existed between roots and leaves in N-deficiency-induced alterations of and C and N metabolisms.
Identifiants
pubmed: 35879653
doi: 10.1186/s12870-022-03759-7
pii: 10.1186/s12870-022-03759-7
pmc: PMC9316421
doi:
Substances chimiques
Carbohydrates
0
Sucrose
57-50-1
Carbon
7440-44-0
Starch
9005-25-8
Nitrogen
N762921K75
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
370Informations de copyright
© 2022. The Author(s).
Références
J Exp Bot. 2002 Feb;53(367):341-50
pubmed: 11807138
Plant Cell Environ. 2015 Apr;38(4):740-50
pubmed: 25159094
Protoplasma. 2017 Nov;254(6):2143-2153
pubmed: 28361178
Int J Mol Sci. 2018 Aug 06;19(8):
pubmed: 30082610
J Exp Bot. 2003 Sep;54(390):2165-75
pubmed: 12885856
Front Plant Sci. 2017 Feb 21;8:185
pubmed: 28270819
J Exp Bot. 2014 Oct;65(19):5535-56
pubmed: 25114014
Trends Plant Sci. 2006 Dec;11(12):610-7
pubmed: 17092760
Tree Physiol. 2016 Jan;36(1):22-38
pubmed: 26420793
Tree Physiol. 2020 Dec 5;40(12):1744-1761
pubmed: 32776117
J Exp Bot. 2012 Sep;63(14):5017-33
pubmed: 22936829
Tree Physiol. 2014 Jun;34(6):608-18
pubmed: 24957048
BMC Plant Biol. 2018 Jun 7;18(1):113
pubmed: 29879900
Molecules. 2011 Jun 29;16(7):5514-26
pubmed: 21716173
Environ Pollut. 2020 Jul;262:114303
pubmed: 32155556
Planta. 2005 Jun;221(3):328-38
pubmed: 15599760
Front Plant Sci. 2018 Nov 20;9:1679
pubmed: 30515181
Plant Physiol. 2012 Nov;160(3):1384-406
pubmed: 22972706
J Exp Bot. 2013 Nov;64(14):4207-24
pubmed: 23963674
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
Trends Plant Sci. 2016 Oct;21(10):811-813
pubmed: 27542324
Plant Physiol Biochem. 2020 Oct;155:455-463
pubmed: 32823246
Mol Plant. 2010 Nov;3(6):973-96
pubmed: 20926550
BMC Plant Biol. 2021 Nov 2;21(1):506
pubmed: 34727870
Phytochemistry. 2013 Apr;88:25-33
pubmed: 23312460
Plant Cell Environ. 2014 Feb;37(2):300-11
pubmed: 23790054
An Acad Bras Cienc. 2020 Nov 11;92(3):e20190254
pubmed: 33206797
Plants (Basel). 2020 Oct 29;9(11):
pubmed: 33137957
Front Plant Sci. 2020 Feb 28;11:187
pubmed: 32184798
J Genet Genomics. 2022 May;49(5):394-404
pubmed: 34973427
Protoplasma. 2020 Mar;257(2):439-447
pubmed: 31748976
Photosynth Res. 1994 Mar;39(3):401-12
pubmed: 24311132
Plant Sci. 2015 Dec;241:55-64
pubmed: 26706058
Plant Cell Physiol. 2008 Jul;49(7):1056-65
pubmed: 18508804
J Exp Bot. 2015 Jun;66(12):3639-55
pubmed: 26038307
J Exp Bot. 2002 Apr;53(370):773-87
pubmed: 11912221
Ann Bot. 2010 Jun;105(7):1141-57
pubmed: 20299346
Ecotoxicol Environ Saf. 2021 Oct 15;223:112579
pubmed: 34352583
Plant J. 2006 May;46(4):533-48
pubmed: 16640592
Front Plant Sci. 2018 Jan 30;9:51
pubmed: 29441082
Front Plant Sci. 2018 Feb 22;9:205
pubmed: 29520286
Plant Cell. 1997 May;9(5):783-798
pubmed: 12237366