Objective Interpretation of the Rapid Urease Test for
Diagnosis
Helicobacter pylori
Rapid Urease Test
Spectrophotometer
Journal
Journal of Korean medical science
ISSN: 1598-6357
Titre abrégé: J Korean Med Sci
Pays: Korea (South)
ID NLM: 8703518
Informations de publication
Date de publication:
25 Jul 2022
25 Jul 2022
Historique:
received:
27
03
2022
accepted:
21
06
2022
entrez:
26
7
2022
pubmed:
27
7
2022
medline:
28
7
2022
Statut:
epublish
Résumé
The rapid urease test (RUT) is a major diagnostic tool for detecting A UV-visible spectrophotometer was selected as the colorimeter; experiments were conducted in three stages to objectively identify the color changes in the RUT kit. First, the urea broth solution showed an identifiable color change from yellow to red as the pH increased by 0.2. The largest transmittance difference detected using the UV-visible spectrophotometer was observed at a 590-nm wavelength. Second, the commercialized RUT kit also showed a gradual color change according to the pH change detected using the UV-visible spectrophotometer. Third, 13 cases of negative RUT results with a biopsy specimen and 16 of positive RUT results were collected. The transmittance detected using the UV-visible spectrophotometer showed a clear division between the positive and negative RUT groups; the largest difference was observed at a 559-nm wavelength. The lowest transmittance in the negative RUT group was 64, while the highest in the positive RUT group was 56, at the 559-nm wavelength. The UV-visible spectrophotometry reading showed a consistency of 92.7% compared with that of manual reading. A transmittance of 60 at a 559-nm wavelength detected using UV-visible spectrophotometer can be used as a cutoff value for interpreting RUT results; this will help develop an automatic RUT kit reader with a high accuracy.
Sections du résumé
BACKGROUND
BACKGROUND
The rapid urease test (RUT) is a major diagnostic tool for detecting
METHODS
METHODS
A UV-visible spectrophotometer was selected as the colorimeter; experiments were conducted in three stages to objectively identify the color changes in the RUT kit.
RESULTS
RESULTS
First, the urea broth solution showed an identifiable color change from yellow to red as the pH increased by 0.2. The largest transmittance difference detected using the UV-visible spectrophotometer was observed at a 590-nm wavelength. Second, the commercialized RUT kit also showed a gradual color change according to the pH change detected using the UV-visible spectrophotometer. Third, 13 cases of negative RUT results with a biopsy specimen and 16 of positive RUT results were collected. The transmittance detected using the UV-visible spectrophotometer showed a clear division between the positive and negative RUT groups; the largest difference was observed at a 559-nm wavelength. The lowest transmittance in the negative RUT group was 64, while the highest in the positive RUT group was 56, at the 559-nm wavelength. The UV-visible spectrophotometry reading showed a consistency of 92.7% compared with that of manual reading.
CONCLUSION
CONCLUSIONS
A transmittance of 60 at a 559-nm wavelength detected using UV-visible spectrophotometer can be used as a cutoff value for interpreting RUT results; this will help develop an automatic RUT kit reader with a high accuracy.
Identifiants
pubmed: 35880503
pii: 37.e227
doi: 10.3346/jkms.2022.37.e227
pmc: PMC9313973
doi:
Substances chimiques
Urease
EC 3.5.1.5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e227Subventions
Organisme : Korean College of Helicobacter and Upper Gastrointestinal Research
ID : KCHUGR-201901005
Pays : Korea
Organisme : Asan Institute for Life Sciences, Asan Medical Center
ID : 2020-00178
Pays : Korea
Organisme : Asan Institute for Life Sciences, Asan Medical Center
ID : A20193988
Pays : Korea
Informations de copyright
© 2022 The Korean Academy of Medical Sciences.
Déclaration de conflit d'intérêts
The authors have no potential conflicts of interest to disclose.
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