The Intracellular Distribution of the Small GTPase Rho5 and Its Dimeric Guanidine Nucleotide Exchange Factor Dck1/Lmo1 Determine Their Function in Oxidative Stress Response.
DNA-Binding Proteins
/ metabolism
Guanidine
Guanine Nucleotide Exchange Factors
/ genetics
Humans
LIM Domain Proteins
/ metabolism
Mitochondrial Proteins
/ metabolism
Monomeric GTP-Binding Proteins
/ metabolism
Nucleotides
/ metabolism
Oxidative Stress
Saccharomyces cerevisiae
/ metabolism
Saccharomyces cerevisiae Proteins
/ metabolism
Transcription Factors
/ metabolism
rho GTP-Binding Proteins
/ genetics
Rho-type GTPase
membrane trapping
mitochondria
oxidative stress
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
18 Jul 2022
18 Jul 2022
Historique:
received:
20
06
2022
revised:
14
07
2022
accepted:
16
07
2022
entrez:
27
7
2022
pubmed:
28
7
2022
medline:
29
7
2022
Statut:
epublish
Résumé
Rho5, the yeast homolog of human Rac1, is a small GTPase which regulates the cell response to nutrient and oxidative stress by inducing mitophagy and apoptosis. It is activated by a dimeric GEF composed of the subunits Dck1 and Lmo1. Upon stress, all three proteins rapidly translocate from the cell surface (Rho5) and a diffuse cytosolic distribution (Dck1 and Lmo1) to mitochondria, with translocation of the GTPase depending on both GEF subunits. We here show that the latter associate with mitochondria independent from each other and from Rho5. The trapping of Dck1-GFP or GFP-Lmo1 to the mitochondrial surface by a specific nanobody fused to the transmembrane domain (TMD) of Fis1 results in a loss of function, mimicking the phenotypes of the respective gene deletions,
Identifiants
pubmed: 35887245
pii: ijms23147896
doi: 10.3390/ijms23147896
pmc: PMC9317549
pii:
doi:
Substances chimiques
DNA-Binding Proteins
0
FIS1 protein, S cerevisiae
0
Guanine Nucleotide Exchange Factors
0
LIM Domain Proteins
0
LMO1 protein, human
0
Mitochondrial Proteins
0
Nucleotides
0
Saccharomyces cerevisiae Proteins
0
Transcription Factors
0
Monomeric GTP-Binding Proteins
EC 3.6.5.2
RHO5 protein, S cerevisiae
EC 3.6.5.2
rho GTP-Binding Proteins
EC 3.6.5.2
Guanidine
JU58VJ6Y3B
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : HE 1880/6-1
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