Interaction of Nucleolin with the Fusion Protein of Avian Metapneumovirus Subgroup C Contributes to Viral Replication.
AS1411
NCL
avian metapneumovirus subgroup C
fusion (F) protein
replication
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
27 06 2022
27 06 2022
Historique:
received:
01
05
2022
revised:
23
06
2022
accepted:
24
06
2022
entrez:
27
7
2022
pubmed:
28
7
2022
medline:
29
7
2022
Statut:
epublish
Résumé
Avian metapneumovirus subgroup C (aMPV/C) is highly pathogenic to various avian species with acute respiratory tract clinicopathology and/or drops in egg production. Nucleolin (NCL), an important nucleolar protein, has been shown to regulate multiple viral replication and serve as a functional receptor for viral entry and internalization. Whether NCL is involved in aMPV/C pathogenesis is not known. In this study, we found that aMPV/C infection altered the subcellular localization of NCL in cultured cells. siRNA-targeted NCL resulted in a remarkable decline in aMPV/C replication in Vero cells. DF-1 cells showed a similar response after CRISPR/Cas9-mediated knock out of NCL during aMPV/C infection. Conversely, NCL overexpression significantly increased aMPV/C replication. Pretreatment with AS1411-a aptamer, a guanine (G)-rich oligonucleotide that forms four-stranded structures and competitively binding to NCL, decreased aMPV/C replication and viral titers in cultured cells. Additionally, we found that the aMPV/C fusion (F) protein specifically interacts with NCL through its central domain and that AS1411 disrupts this interaction, thus inhibiting viral replication. Taken together, these results reveal that the aMPV/C F protein interacts with NCL, which is employed by aMPV/C for efficient replication, thereby highlighting the strategic potential for control and therapy of aMPV/C infection.
Identifiants
pubmed: 35891383
pii: v14071402
doi: 10.3390/v14071402
pmc: PMC9317408
pii:
doi:
Substances chimiques
Phosphoproteins
0
RNA-Binding Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Références
Vet J. 2000 Sep;160(2):118-25
pubmed: 10985803
J Biol Chem. 2000 Feb 11;275(6):4152-8
pubmed: 10660576
Biochemistry. 1987 Dec 1;26(24):7876-83
pubmed: 3427111
RNA Biol. 2012 Jun;9(6):799-808
pubmed: 22617883
Nucleic Acids Res. 2012 Oct;40(19):9441-54
pubmed: 22859736
J Virol. 2001 Jan;75(2):1031-8
pubmed: 11134316
J Biol Chem. 1997 May 16;272(20):13109-16
pubmed: 9148924
Cell. 2005 Oct 7;123(1):49-63
pubmed: 16213212
EMBO J. 2000 Dec 15;19(24):6870-81
pubmed: 11118222
Virology. 2016 Jul;494:78-88
pubmed: 27085069
J Biol Chem. 1987 Aug 15;262(23):10922-5
pubmed: 2440879
Eur J Biochem. 1982 Nov 15;128(2-3):475-80
pubmed: 7151790
Viruses. 2020 Mar 28;12(4):
pubmed: 32231136
Avian Pathol. 1996 Dec;25(4):639-48
pubmed: 18645888
J Gen Virol. 1975 Feb;26(2):183-96
pubmed: 163289
Exp Cell Res. 1983 Jun;146(1):139-49
pubmed: 6345184
J Virol. 2000 Jul;74(14):6442-7
pubmed: 10864656
Vaccine. 2002 Jan 15;20(7-8):1058-67
pubmed: 11803066
J Virol. 1998 Aug;72(8):6699-709
pubmed: 9658117
J Virol. 2000 Nov;74(22):10508-13
pubmed: 11044095
Int J Antimicrob Agents. 2016 Apr;47(4):311-6
pubmed: 27032748
Nat Med. 2011 Aug 14;17(9):1132-5
pubmed: 21841784
J Virol. 2013 Dec;87(24):13094-106
pubmed: 24027323
Biochimie. 2015 Jun;113:78-85
pubmed: 25866190
J Virol. 2021 Jun 10;95(13):e0023821
pubmed: 33853964
J Biol Chem. 1998 Nov 27;273(48):31718-25
pubmed: 9822633
Exp Mol Pathol. 2009 Jun;86(3):151-64
pubmed: 19454272
Biochemistry. 1985 Oct 22;24(22):6025-8
pubmed: 4084504
EMBO J. 2006 Apr 19;25(8):1669-79
pubmed: 16601700
PLoS One. 2016 Oct 6;11(10):e0164146
pubmed: 27711134
J Gen Virol. 2000 Nov;81(Pt 11):2723-2733
pubmed: 11038385
PLoS Pathog. 2018 Oct 19;14(10):e1007383
pubmed: 30339712
Crit Rev Biochem Mol Biol. 1998;33(6):407-36
pubmed: 9918513
Somat Cell Mol Genet. 1998 Sep;24(5):263-72
pubmed: 10696234
Mol Biol Rep. 1983 May;9(1-2):39-47
pubmed: 6350846
J Virol. 2015 Apr;89(8):4527-38
pubmed: 25673703
J Biol Chem. 1994 May 20;269(20):14465-72
pubmed: 8182051
Vet Rec. 1986 Feb 22;118(8):206-7
pubmed: 3716162
Exp Cell Res. 1981 Nov;136(1):101-9
pubmed: 7028492
Clin Microbiol Rev. 2004 Apr;17(2):390-412
pubmed: 15084507
Eur J Biochem. 1992 Oct 15;209(2):541-8
pubmed: 1425660
EMBO J. 1998 Mar 2;17(5):1476-86
pubmed: 9482744
Virology. 2000 Jun 5;271(2):264-75
pubmed: 10860881
Autophagy. 2017 Oct 3;13(10):1709-1721
pubmed: 28949785
Viruses. 2017 Aug 03;9(8):
pubmed: 28771197
J Biol Chem. 2012 May 11;287(20):16467-76
pubmed: 22433872
PLoS One. 2009 Dec 15;4(12):e8325
pubmed: 20016834
Arch Virol. 2018 Oct;163(10):2775-2786
pubmed: 29974255
Emerg Infect Dis. 2013 Jul;19(7):1092-4
pubmed: 23763901
Ann N Y Acad Sci. 2007 Apr;1102:66-85
pubmed: 17470912
J Cell Sci. 1999 Mar;112 ( Pt 6):761-72
pubmed: 10036227
J Biol Chem. 1986 Jul 15;261(20):9167-73
pubmed: 3755137
Virology. 2016 Feb;489:51-62
pubmed: 26707270
J Virol. 2014 Oct;88(20):11738-47
pubmed: 25078694
FEBS Lett. 2011 Feb 18;585(4):618-22
pubmed: 21281639
Mol Cell. 2011 Oct 7;44(1):108-19
pubmed: 21981922
Mol Biol Rep. 1996;23(3-4):191-5
pubmed: 9112228
J Gen Virol. 2020 Jan;101(1):33-43
pubmed: 31794379