PIWI Proteins Play an Antiviral Role in Lepidopteran Cell Lines.

Ago3 Argonaute Bombyx mori Macula-like Latent Virus (MLV) Cricket Paralysis Virus (CrPV) Flock House Virus (FHV) RNAi Siwi Spodoptera frugiperda Rhabdovirus (RV) immunity insect piRNA sRNA siRNA virus

Journal

Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722

Informations de publication

Date de publication:
30 06 2022
Historique:
received: 08 06 2022
revised: 27 06 2022
accepted: 27 06 2022
entrez: 27 7 2022
pubmed: 28 7 2022
medline: 29 7 2022
Statut: epublish

Résumé

Insect antiviral immunity primarily relies on RNAi mechanisms. While a key role of small interfering (si)RNAs and AGO proteins has been well established in this regard, the situation for PIWI proteins and PIWI-interacting (pi)RNAs is not as clear. In the present study, we investigate whether PIWI proteins and viral piRNAs are involved in the immunity against single-stranded RNA viruses in lepidopteran cells, where two PIWIs are identified (Siwi and Ago3). Via loss- and gain-of-function studies in

Identifiants

pubmed: 35891422
pii: v14071442
doi: 10.3390/v14071442
pmc: PMC9321812
pii:
doi:

Substances chimiques

Antiviral Agents 0
Argonaute Proteins 0
RNA, Small Interfering 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Dulce Santos (D)

Research Group of Molecular Developmental Physiology and Signal Transduction, Division of Animal Physiology and Neurobiology, Department of Biology, KU Leuven, Naamsestraat 59, 3000 Leuven, Belgium.

Thomas-Wolf Verdonckt (TW)

Research Group of Molecular Developmental Physiology and Signal Transduction, Division of Animal Physiology and Neurobiology, Department of Biology, KU Leuven, Naamsestraat 59, 3000 Leuven, Belgium.

Lina Mingels (L)

Research Group of Molecular Developmental Physiology and Signal Transduction, Division of Animal Physiology and Neurobiology, Department of Biology, KU Leuven, Naamsestraat 59, 3000 Leuven, Belgium.

Stijn Van den Brande (S)

Research Group of Molecular Developmental Physiology and Signal Transduction, Division of Animal Physiology and Neurobiology, Department of Biology, KU Leuven, Naamsestraat 59, 3000 Leuven, Belgium.

Bart Geens (B)

Research Group of Molecular Developmental Physiology and Signal Transduction, Division of Animal Physiology and Neurobiology, Department of Biology, KU Leuven, Naamsestraat 59, 3000 Leuven, Belgium.

Filip Van Nieuwerburgh (F)

Laboratory of Pharmaceutical Biotechnology, Gent University, Ottergemsesteenweg 460, 9000 Gent, Belgium.

Anna Kolliopoulou (A)

Insect Molecular Genetics and Biotechnology, Institute of Biosciences and Applications, National Center for Scientific Research "Demokritos", Aghia Paraskevi Attikis, 153 10 Athens, Greece.

Luc Swevers (L)

Insect Molecular Genetics and Biotechnology, Institute of Biosciences and Applications, National Center for Scientific Research "Demokritos", Aghia Paraskevi Attikis, 153 10 Athens, Greece.

Niels Wynant (N)

Research Group of Molecular Developmental Physiology and Signal Transduction, Division of Animal Physiology and Neurobiology, Department of Biology, KU Leuven, Naamsestraat 59, 3000 Leuven, Belgium.

Jozef Vanden Broeck (J)

Research Group of Molecular Developmental Physiology and Signal Transduction, Division of Animal Physiology and Neurobiology, Department of Biology, KU Leuven, Naamsestraat 59, 3000 Leuven, Belgium.

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