In vitro effect on the proteolytic activity of papain with proteins of the skin as substrate.
biotechnology
formulation/stability
skin structure
statistics
Journal
International journal of cosmetic science
ISSN: 1468-2494
Titre abrégé: Int J Cosmet Sci
Pays: England
ID NLM: 8007161
Informations de publication
Date de publication:
Oct 2022
Oct 2022
Historique:
revised:
20
07
2022
received:
29
06
2022
accepted:
25
07
2022
pubmed:
28
7
2022
medline:
15
9
2022
entrez:
27
7
2022
Statut:
ppublish
Résumé
This work aims to evaluate the effects of enzyme concentration, pH, temperature and time course degree of hydrolysis (DH) of papain regarding further development of pharmaceutical and cosmetic formulations. The hydrolysis of casein, collagen, keratin and porcine skin at pH and temperature ranges of the human skin was evaluated. Also, low contact times of enzyme-substrate were studied. The incorporation of 3mM of cysteine improved the caseinolytic (PU), collagenolytic (CU) and keratinolytic (KU) activities of papain. In general, the increase from 0.1 to 1.0 or 2.0 mgmL This preformulation study demonstrated that papain concentration, time of application and pH of the product should be evaluated when developing a product to promote the hydrolysis of the proteins of the skin. Ce travail vise à évaluer les effets de la concentration d’enzyme, du pH, de la température et du degré d’hydrolyse (DH) de la papaïne concernant le développement ultérieur de formulations pharmaceutiques et cosmétiques. MÉTHODES: L'hydrolyse de la caséine, du collagène, de la kératine et de la peau porcine à des plages de pH et de température de la peau humaine a été évaluée. De plus, des faibles temps de contact enzyme-substrat ont été étudiés. L'incorporation de 3mM de cystéine a amélioré les activités caséinolytiques (PU), collagénolytiques (CU) et kératinolytiques (KU) de la papaïne. RÉSULTATS: En général, l'augmentation de 0,1 à 1,0 ou 2,0 mg.mL Cette étude de pré-formulation a démontré que la concentration de papaïne, le temps d’application et le pH du produit doivent être évalués lors du développement d'un produit pour favoriser l'hydrolyse des protéines de la peau.
Autres résumés
Type: Publisher
(fre)
Ce travail vise à évaluer les effets de la concentration d’enzyme, du pH, de la température et du degré d’hydrolyse (DH) de la papaïne concernant le développement ultérieur de formulations pharmaceutiques et cosmétiques. MÉTHODES: L'hydrolyse de la caséine, du collagène, de la kératine et de la peau porcine à des plages de pH et de température de la peau humaine a été évaluée. De plus, des faibles temps de contact enzyme-substrat ont été étudiés. L'incorporation de 3mM de cystéine a amélioré les activités caséinolytiques (PU), collagénolytiques (CU) et kératinolytiques (KU) de la papaïne. RÉSULTATS: En général, l'augmentation de 0,1 à 1,0 ou 2,0 mg.mL
Substances chimiques
Caseins
0
Collagen
9007-34-5
Keratins
68238-35-7
Papain
EC 3.4.22.2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
542-554Subventions
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : 001
Informations de copyright
© 2022 Society of Cosmetic Scientists and Societe Francaise de Cosmetologie.
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