High-Throughput CRISPR Screening in Hematological Neoplasms.
CRISPR
algorithms
hematological neoplasms
libraries
resistances
vulnerabilities
Journal
Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829
Informations de publication
Date de publication:
25 Jul 2022
25 Jul 2022
Historique:
received:
29
06
2022
revised:
16
07
2022
accepted:
20
07
2022
entrez:
27
7
2022
pubmed:
28
7
2022
medline:
28
7
2022
Statut:
epublish
Résumé
CRISPR is becoming an indispensable tool in biological research, revolutionizing diverse fields of medical research and biotechnology. In the last few years, several CRISPR-based genome-targeting tools have been translated for the study of hematological neoplasms. However, there is a lack of reviews focused on the wide uses of this technology in hematology. Therefore, in this review, we summarize the main CRISPR-based approaches of high throughput screenings applied to this field. Here we explain several libraries and algorithms for analysis of CRISPR screens used in hematology, accompanied by the most relevant databases. Moreover, we focus on (1) the identification of novel modulator genes of drug resistance and efficacy, which could anticipate relapses in patients and (2) new therapeutic targets and synthetic lethal interactions. We also discuss the approaches to uncover novel biomarkers of malignant transformations and immune evasion mechanisms. We explain the current literature in the most common lymphoid and myeloid neoplasms using this tool. Then, we conclude with future directions, highlighting the importance of further gene candidate validation and the integration and harmonization of the data from CRISPR screening approaches.
Identifiants
pubmed: 35892871
pii: cancers14153612
doi: 10.3390/cancers14153612
pmc: PMC9329962
pii:
doi:
Types de publication
Journal Article
Review
Langues
eng
Subventions
Organisme : AECC
ID : Ideas Semilla Award
Organisme : CRIS foundation
ID : CRIS
Organisme : Ministry of Science, Innovation and Universities of Spain Government
ID : FPU19/04933
Organisme : Community of Madrid (CAM)
ID : CAM
Organisme : CAM-REACT grant
ID : CAM-REACT grant
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