In-Depth Characterization of

CRISPR-Cas9 breeding greenflesh nutritional quality pathogen resistance staygreen tomato

Journal

Frontiers in plant science
ISSN: 1664-462X
Titre abrégé: Front Plant Sci
Pays: Switzerland
ID NLM: 101568200

Informations de publication

Date de publication:
2022
Historique:
received: 04 05 2022
accepted: 15 06 2022
entrez: 28 7 2022
pubmed: 29 7 2022
medline: 29 7 2022
Statut: epublish

Résumé

Gene editing has already proved itself as an invaluable tool for the generation of mutants for crop breeding, yet its ultimate impact on agriculture will depend on how crops generated by gene editing technologies are regulated, and on our ability to characterize the impact of mutations on plant phenotype. A starting operational strategy for evaluating gene editing-based approaches to plant breeding might consist of assessing the effect of the induced mutations in a crop- and locus-specific manner: this involves the analysis of editing efficiency in different cultivars of a crop, the assessment of potential off-target mutations, and a phenotypic evaluation of edited lines carrying different mutated alleles. Here, we targeted the

Identifiants

pubmed: 35898224
doi: 10.3389/fpls.2022.936089
pmc: PMC9309892
doi:

Types de publication

Journal Article

Langues

eng

Pagination

936089

Informations de copyright

Copyright © 2022 Gianoglio, Comino, Moglia, Acquadro, García-Carpintero, Diretto, Sevi, Rambla, Dono, Valentino, Moreno-Giménez, Fullana-Pericàs, Conesa, Galmés, Lanteri, Mazzucato, Orzáez and Granell.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Silvia Gianoglio (S)

Departamento de Biotecnología de Cultivos, Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas (CSIC) - Universitat Politécnica de Valéncia (UPV), Valencia, Spain.

Cinzia Comino (C)

Dipartimento di Scienze Agrarie, Forestali e Alimentari (DISAFA), Plant Genetics and Breeding, University of Turin, Turin, Italy.

Andrea Moglia (A)

Dipartimento di Scienze Agrarie, Forestali e Alimentari (DISAFA), Plant Genetics and Breeding, University of Turin, Turin, Italy.

Alberto Acquadro (A)

Dipartimento di Scienze Agrarie, Forestali e Alimentari (DISAFA), Plant Genetics and Breeding, University of Turin, Turin, Italy.

Víctor García-Carpintero (V)

Departamento de Biotecnología de Cultivos, Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas (CSIC) - Universitat Politécnica de Valéncia (UPV), Valencia, Spain.

Gianfranco Diretto (G)

Italian Agency for New Technologies, Energy and Sustainable Development (ENEA), Rome, Italy.

Filippo Sevi (F)

Italian Agency for New Technologies, Energy and Sustainable Development (ENEA), Rome, Italy.
Department of Agricultural Sciences, University of Naples Federico II, Naples, Italy.

José Luis Rambla (JL)

Departamento de Biotecnología de Cultivos, Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas (CSIC) - Universitat Politécnica de Valéncia (UPV), Valencia, Spain.
Department of Biology, Biochemistry and Natural Sciences, Universitat Jaume I, Castellón de la Plana, Spain.

Gabriella Dono (G)

Department of Agriculture and Forest Sciences (DAFNE), Università degli Studi della Tuscia, Viterbo, Italy.

Danila Valentino (D)

Dipartimento di Scienze Agrarie, Forestali e Alimentari (DISAFA), Plant Genetics and Breeding, University of Turin, Turin, Italy.

Elena Moreno-Giménez (E)

Departamento de Biotecnología de Cultivos, Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas (CSIC) - Universitat Politécnica de Valéncia (UPV), Valencia, Spain.
Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de los Alimentos (IATA-CSIC), Paterna, Spain.

Mateu Fullana-Pericàs (M)

Instituto de Investigaciones Agroambientales y de Economía del Agua (INAGEA), Research Group on Plant Biology Under Mediterranean Conditions, Universitat de les Illes Balears, Palma, Spain.

Miguel A Conesa (MA)

Instituto de Investigaciones Agroambientales y de Economía del Agua (INAGEA), Research Group on Plant Biology Under Mediterranean Conditions, Universitat de les Illes Balears, Palma, Spain.

Jeroni Galmés (J)

Instituto de Investigaciones Agroambientales y de Economía del Agua (INAGEA), Research Group on Plant Biology Under Mediterranean Conditions, Universitat de les Illes Balears, Palma, Spain.

Sergio Lanteri (S)

Dipartimento di Scienze Agrarie, Forestali e Alimentari (DISAFA), Plant Genetics and Breeding, University of Turin, Turin, Italy.

Andrea Mazzucato (A)

Department of Agriculture and Forest Sciences (DAFNE), Università degli Studi della Tuscia, Viterbo, Italy.

Diego Orzáez (D)

Departamento de Biotecnología de Cultivos, Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas (CSIC) - Universitat Politécnica de Valéncia (UPV), Valencia, Spain.

Antonio Granell (A)

Departamento de Biotecnología de Cultivos, Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas (CSIC) - Universitat Politécnica de Valéncia (UPV), Valencia, Spain.

Classifications MeSH