Determination of thick and thin fibres distribution in Sunda porcupine dorsal skin (Hystrix javanica) using Picrosirius red staining.
Sunda porcupine
collagen
picrosirius red
polarized light
quill follicle
Journal
Anatomia, histologia, embryologia
ISSN: 1439-0264
Titre abrégé: Anat Histol Embryol
Pays: Germany
ID NLM: 7704218
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
revised:
07
07
2022
received:
22
04
2022
accepted:
13
07
2022
pubmed:
29
7
2022
medline:
14
9
2022
entrez:
28
7
2022
Statut:
ppublish
Résumé
The complexity of the Sunda porcupine skin has become an important topic due to the unique characteristics of its quill follicles. The structure and chemical composition of the skin has affected many physiological and other conditions. Generally, quills are larger, stronger and stiffer than hair; therefore, the skin structure needs to adapt to support their physiology. The strength of the skin is determined by its collagen composition and arrangement; therefore, this study aims to analyse the composition and distribution of thick and thin fibres based on the specific characteristics of Sunda porcupine skin under polarized light using picrosirius red staining. The skin samples used were from the thoracodorsal and lumbosacral regions of eight Sunda porcupine adults. The histological staining was carried out using the picrosirius red method, while the samples were observed under a polarized light microscope and analysed with software. The results showed that the skin is composed of 36%-65% thick fibres, 20%-35% thin fibres and small amounts of other types with the lumbosacral region having higher compositions of thick and thin fibres than those in the thoracodorsal region. Furthermore, the thoracodorsal and lumbosacral regions have the highest composition of thick fibre in the deeper dermis and quill follicle, respectively. These demonstrated that the complexity of the skin structure of Sunda porcupine due to its quill derivates correlated with its collagen composition and distribution.
Substances chimiques
Azo Compounds
0
C.I. direct red 80
1294D5G72N
Collagen
9007-34-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
666-673Subventions
Organisme : Kementerian Pendidikan dan Kebudayaan
Organisme : Kementerian Riset Teknologi Dan Pendidikan Tinggi Republik Indonesia
Informations de copyright
© 2022 Wiley-VCH GmbH.
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