Higher-order structure of barley chromosomes observed by electron tomography.
Barley chromosomes
Electron tomography
TEM
Journal
Micron (Oxford, England : 1993)
ISSN: 1878-4291
Titre abrégé: Micron
Pays: England
ID NLM: 9312850
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
received:
06
04
2022
revised:
11
07
2022
accepted:
11
07
2022
pubmed:
30
7
2022
medline:
12
8
2022
entrez:
29
7
2022
Statut:
ppublish
Résumé
The higher order structure of the metaphase chromosome has been an enigma for over a century and several different models have been presented based on results obtained by a variety of techniques. Some disagreements in the results between methods have possibly arisen from artifacts caused during sample preparation such as staining and dehydration. Therefore, we treated barley chromosomes with ionic liquid to minimize the effects of dehydration. We also observed chromosomes on a film with holes to keep pristine chromosome structure from being flattened as seen when placed on a continuous support film. A chromosome placed over a hole in a thin carbon film was mounted on a tomography holder, and its structure was observed in three dimensions (3D) using electron tomography. We found that there are periodic structures with 300-400 nm pitch along the axis in barley chromosomes. The pitch sizes are larger than those observed in human chromosomes.
Identifiants
pubmed: 35905587
pii: S0968-4328(22)00124-X
doi: 10.1016/j.micron.2022.103328
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
103328Informations de copyright
Copyright © 2022. Published by Elsevier Ltd.